The VpreB1 enhancer drives developmental stage-specific gene expression in vivo
(2003) In European Journal of Immunology 33(4). p.1117-1126- Abstract
- In adult mice, the VpreB genes are expressed in bone marrow progenitor (pro-) and precursor (pre-) B cells. As part of the pre-B cell receptor, the proteins are crucial for the proliferation of these cells and consequently normal B lymphocyte development. Using cell lines, we identified a lineage- and developmental-stage-specific VpreB1 enhancer. Here, we analyze its specificity in vivo by generating transgenic mice in which expression of a reporter gene (human CD122) is regulated by the VpreB1 enhancer in the context of its own promoter. All transgenic lines expressed the reporter gene in the bone marrow in a copy number-independent manner, whereas expression levels were integration site-dependent. While the enhancer is not tissue... (More)
- In adult mice, the VpreB genes are expressed in bone marrow progenitor (pro-) and precursor (pre-) B cells. As part of the pre-B cell receptor, the proteins are crucial for the proliferation of these cells and consequently normal B lymphocyte development. Using cell lines, we identified a lineage- and developmental-stage-specific VpreB1 enhancer. Here, we analyze its specificity in vivo by generating transgenic mice in which expression of a reporter gene (human CD122) is regulated by the VpreB1 enhancer in the context of its own promoter. All transgenic lines expressed the reporter gene in the bone marrow in a copy number-independent manner, whereas expression levels were integration site-dependent. While the enhancer is not tissue specific, within the B cell lineage the expression pattern of human CID122 mimicked that of endogenous VpreB1. Thus, low levels were detected in pro-B cells, high levels in pre-BI and slightly lower levels in pre-BII cells; no expression was detected in immature/mature B cells. Furthermore, when in vitro cultured transgenic pre-B cells differentiated into immature B cells there was concomitant down-regulation of human CD122 and endogenous VpreB1. Thus the VpreB1 enhancer is sufficient to ensure developmental stage-specific expression of a reporter gene in B lymphocytes in vivo. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/313779
- author
- Licence, S ; Persson, Christine LU ; Mundt, C and Martensson, IL
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- pre-B cell, surrogate light chain, VpreB, enhancer
- in
- European Journal of Immunology
- volume
- 33
- issue
- 4
- pages
- 1117 - 1126
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000182186500032
- pmid:12672078
- scopus:0038744372
- ISSN
- 1521-4141
- DOI
- 10.1002/eji.200323702
- language
- English
- LU publication?
- yes
- id
- 9e528209-0aa6-4f4b-9375-2b1370c308c3 (old id 313779)
- date added to LUP
- 2016-04-01 11:49:48
- date last changed
- 2022-04-20 22:25:19
@article{9e528209-0aa6-4f4b-9375-2b1370c308c3,
abstract = {{In adult mice, the VpreB genes are expressed in bone marrow progenitor (pro-) and precursor (pre-) B cells. As part of the pre-B cell receptor, the proteins are crucial for the proliferation of these cells and consequently normal B lymphocyte development. Using cell lines, we identified a lineage- and developmental-stage-specific VpreB1 enhancer. Here, we analyze its specificity in vivo by generating transgenic mice in which expression of a reporter gene (human CD122) is regulated by the VpreB1 enhancer in the context of its own promoter. All transgenic lines expressed the reporter gene in the bone marrow in a copy number-independent manner, whereas expression levels were integration site-dependent. While the enhancer is not tissue specific, within the B cell lineage the expression pattern of human CID122 mimicked that of endogenous VpreB1. Thus, low levels were detected in pro-B cells, high levels in pre-BI and slightly lower levels in pre-BII cells; no expression was detected in immature/mature B cells. Furthermore, when in vitro cultured transgenic pre-B cells differentiated into immature B cells there was concomitant down-regulation of human CD122 and endogenous VpreB1. Thus the VpreB1 enhancer is sufficient to ensure developmental stage-specific expression of a reporter gene in B lymphocytes in vivo.}},
author = {{Licence, S and Persson, Christine and Mundt, C and Martensson, IL}},
issn = {{1521-4141}},
keywords = {{pre-B cell; surrogate light chain; VpreB; enhancer}},
language = {{eng}},
number = {{4}},
pages = {{1117--1126}},
publisher = {{John Wiley & Sons Inc.}},
series = {{European Journal of Immunology}},
title = {{The VpreB1 enhancer drives developmental stage-specific gene expression in vivo}},
url = {{http://dx.doi.org/10.1002/eji.200323702}},
doi = {{10.1002/eji.200323702}},
volume = {{33}},
year = {{2003}},
}