Valoración de una novedosa tecnología para la detección de enterovirus en aguas negras
(2000) In Revista cubana de medicina tropical 51(3). p.71-166- Abstract
This paper reported the appraisal of a novel technology for the detection of enterovirus in sewage based on a viral recovery method using polyethylenglycol as a concentrating agent and on the combined use of viral isolation and polymerase chain reaction as viral detection and identification techniques. It was also confirmed that the viral recovery method is highly efficient since it allows to recover all viruses present in sewage and to preserve their infective capacity. It was proved that the polymerase chain reaction (PCR) is less sensitive than cell culture for detecting enteroviruses in sewage. It is also possible to use guadinine isothiocyanate, whose components can be prepared in the lab, as an alternative method for taking out... (More)
This paper reported the appraisal of a novel technology for the detection of enterovirus in sewage based on a viral recovery method using polyethylenglycol as a concentrating agent and on the combined use of viral isolation and polymerase chain reaction as viral detection and identification techniques. It was also confirmed that the viral recovery method is highly efficient since it allows to recover all viruses present in sewage and to preserve their infective capacity. It was proved that the polymerase chain reaction (PCR) is less sensitive than cell culture for detecting enteroviruses in sewage. It is also possible to use guadinine isothiocyanate, whose components can be prepared in the lab, as an alternative method for taking out and purifying nucleic acids instead of using the conventional TRIZOL method which is the one recommended in these cases by the World Health Organization.
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- author
- Sarmiento Pérez, L LU ; Más Lago, P ; Avalos Redón, I ; Palomera Puentes, R ; Barrios Olivera, J and Bello Corredor, M
- alternative title
- Evaluation of a new technology for the detection of enterovirus in sewage
- publishing date
- 2000-07-11
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Enterovirus/isolation & purification, Evaluation Studies as Topic, Sewage/virology, Virology/methods
- in
- Revista cubana de medicina tropical
- volume
- 51
- issue
- 3
- pages
- 6 pages
- publisher
- Editorial Ciencias Medicas
- external identifiers
-
- scopus:0033186395
- pmid:10887582
- ISSN
- 0375-0760
- language
- Spanish
- LU publication?
- no
- id
- 9e66a6c9-2085-4f01-b9ef-de69317133f0
- alternative location
- http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375-07601999000300005
- date added to LUP
- 2019-07-08 15:15:50
- date last changed
- 2024-01-01 15:46:21
@article{9e66a6c9-2085-4f01-b9ef-de69317133f0,
abstract = {{<p>This paper reported the appraisal of a novel technology for the detection of enterovirus in sewage based on a viral recovery method using polyethylenglycol as a concentrating agent and on the combined use of viral isolation and polymerase chain reaction as viral detection and identification techniques. It was also confirmed that the viral recovery method is highly efficient since it allows to recover all viruses present in sewage and to preserve their infective capacity. It was proved that the polymerase chain reaction (PCR) is less sensitive than cell culture for detecting enteroviruses in sewage. It is also possible to use guadinine isothiocyanate, whose components can be prepared in the lab, as an alternative method for taking out and purifying nucleic acids instead of using the conventional TRIZOL method which is the one recommended in these cases by the World Health Organization.</p>}},
author = {{Sarmiento Pérez, L and Más Lago, P and Avalos Redón, I and Palomera Puentes, R and Barrios Olivera, J and Bello Corredor, M}},
issn = {{0375-0760}},
keywords = {{Enterovirus/isolation & purification; Evaluation Studies as Topic; Sewage/virology; Virology/methods}},
language = {{spa}},
month = {{07}},
number = {{3}},
pages = {{71--166}},
publisher = {{Editorial Ciencias Medicas}},
series = {{Revista cubana de medicina tropical}},
title = {{Valoración de una novedosa tecnología para la detección de enterovirus en aguas negras}},
url = {{http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375-07601999000300005}},
volume = {{51}},
year = {{2000}},
}