Prussian blue-glutamate oxidase modified glassy carbon electrode: A sensitive L-glutamate and beta-N-oxalyl-alpha-beta-diaminopropionic acid (beta-ODAP) sensor
(2006) In Analytica Chimica Acta 556(2). p.319-325- Abstract
- A sensitive biosensor has been developed for the neurotoxin beta-N-oxalyl-alpha,beta-diaminopropionic acid (beta-ODAP) contained in the seeds of grass pea (Lathyrus sativus) and for L-glutamate based on glutamate oxidase (GlOx) and a Prussian blue (PB) modified glassy carbon (GC) electrode. The configuration of the system is so as to detect the hydrogen peroxide released during the enzymatic cycle at a low applied potential, -50 mV versus Ag vertical bar AgCl, in the flow injection mode. For this purpose GlOx was coupled to PB electrodeposited onto a glassy carbon electrode and stabilised by treatment with tetrabutylammonium toluene-4-sulfonate (TTS) during one of the steps in the electrodeposition. GlOx was cross-linked with... (More)
- A sensitive biosensor has been developed for the neurotoxin beta-N-oxalyl-alpha,beta-diaminopropionic acid (beta-ODAP) contained in the seeds of grass pea (Lathyrus sativus) and for L-glutamate based on glutamate oxidase (GlOx) and a Prussian blue (PB) modified glassy carbon (GC) electrode. The configuration of the system is so as to detect the hydrogen peroxide released during the enzymatic cycle at a low applied potential, -50 mV versus Ag vertical bar AgCl, in the flow injection mode. For this purpose GlOx was coupled to PB electrodeposited onto a glassy carbon electrode and stabilised by treatment with tetrabutylammonium toluene-4-sulfonate (TTS) during one of the steps in the electrodeposition. GlOx was cross-linked with glutaraldehyde (GA), bovine serum albumin (BSA) and Tween-20 on the surface of the PB modified GC electrodes. Addition of 0.01% and 0.001% polyethyleneimine (PEI) to the immobilisation mixture resulted in an enhancement of the response signal with about 35% and 62% for glutamate and P-ODAP, respectively, when using 0.01% PEI and with 164% and 200% for glutamate and P-ODAP, respectively, when using 0.001% PEI. The linear response range for P-ODAP was extended from 0.05-0.5 mM to 0.01-1 mM, when 0.001% PEI was used. However, a higher concentration of PEI, 0.1%, caused a decrease in the sensitivity of the biosensor. (c) 2005 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/419999
- author
- Varma, Shailly LU ; Yigzaw, Yirgalem LU and Gorton, Lo LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Prussian blue, electrodes, enzyme modified, polyethyleneimine, glutamate oxidase, beta-ODAP
- in
- Analytica Chimica Acta
- volume
- 556
- issue
- 2
- pages
- 319 - 325
- publisher
- Elsevier
- external identifiers
-
- wos:000234729700010
- scopus:29944441490
- ISSN
- 1873-4324
- DOI
- 10.1016/j.aca.2005.09.047
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Department of Chemistry (011001220)
- id
- 9e83a1e9-23d2-48ca-9316-48fa918d74ae (old id 419999)
- date added to LUP
- 2016-04-01 16:37:04
- date last changed
- 2022-01-28 20:55:57
@article{9e83a1e9-23d2-48ca-9316-48fa918d74ae, abstract = {{A sensitive biosensor has been developed for the neurotoxin beta-N-oxalyl-alpha,beta-diaminopropionic acid (beta-ODAP) contained in the seeds of grass pea (Lathyrus sativus) and for L-glutamate based on glutamate oxidase (GlOx) and a Prussian blue (PB) modified glassy carbon (GC) electrode. The configuration of the system is so as to detect the hydrogen peroxide released during the enzymatic cycle at a low applied potential, -50 mV versus Ag vertical bar AgCl, in the flow injection mode. For this purpose GlOx was coupled to PB electrodeposited onto a glassy carbon electrode and stabilised by treatment with tetrabutylammonium toluene-4-sulfonate (TTS) during one of the steps in the electrodeposition. GlOx was cross-linked with glutaraldehyde (GA), bovine serum albumin (BSA) and Tween-20 on the surface of the PB modified GC electrodes. Addition of 0.01% and 0.001% polyethyleneimine (PEI) to the immobilisation mixture resulted in an enhancement of the response signal with about 35% and 62% for glutamate and P-ODAP, respectively, when using 0.01% PEI and with 164% and 200% for glutamate and P-ODAP, respectively, when using 0.001% PEI. The linear response range for P-ODAP was extended from 0.05-0.5 mM to 0.01-1 mM, when 0.001% PEI was used. However, a higher concentration of PEI, 0.1%, caused a decrease in the sensitivity of the biosensor. (c) 2005 Elsevier B.V. All rights reserved.}}, author = {{Varma, Shailly and Yigzaw, Yirgalem and Gorton, Lo}}, issn = {{1873-4324}}, keywords = {{Prussian blue; electrodes; enzyme modified; polyethyleneimine; glutamate oxidase; beta-ODAP}}, language = {{eng}}, number = {{2}}, pages = {{319--325}}, publisher = {{Elsevier}}, series = {{Analytica Chimica Acta}}, title = {{Prussian blue-glutamate oxidase modified glassy carbon electrode: A sensitive L-glutamate and beta-N-oxalyl-alpha-beta-diaminopropionic acid (beta-ODAP) sensor}}, url = {{http://dx.doi.org/10.1016/j.aca.2005.09.047}}, doi = {{10.1016/j.aca.2005.09.047}}, volume = {{556}}, year = {{2006}}, }