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ABCC3 is a novel target for the treatment of pancreatic cancer

Adamska, Aleksandra LU ; Ferro, Riccardo ; Lattanzio, Rossano ; Capone, Emily ; Domenichini, Alice ; Damiani, Verena ; Chiorino, Giovanna ; Akkaya, Begum Gokcen ; Linton, Kenneth J and De Laurenzi, Vincenzo , et al. (2019) In Advances in Biological Regulation 73.
Abstract

Pancreatic Ductal Adenocarcinoma (PDAC) is a very aggressive disease, lacking effective therapeutic approaches and leaving PDAC patients with a poor prognosis. The life expectancy of PDAC patients has not experienced a significant change in the last few decades with a five-year survival rate of only 8%. To address this unmet need, novel pharmacological targets must be identified for clinical intervention. ATP Binding Cassette (ABC) transporters are frequently overexpressed in different cancer types and represent one of the major mechanisms responsible for chemoresistance. However, a more direct role for ABC transporters in tumorigenesis has not been widely investigated. Here, we show that ABCC3 (ABC Subfamily C Member 3; previously... (More)

Pancreatic Ductal Adenocarcinoma (PDAC) is a very aggressive disease, lacking effective therapeutic approaches and leaving PDAC patients with a poor prognosis. The life expectancy of PDAC patients has not experienced a significant change in the last few decades with a five-year survival rate of only 8%. To address this unmet need, novel pharmacological targets must be identified for clinical intervention. ATP Binding Cassette (ABC) transporters are frequently overexpressed in different cancer types and represent one of the major mechanisms responsible for chemoresistance. However, a more direct role for ABC transporters in tumorigenesis has not been widely investigated. Here, we show that ABCC3 (ABC Subfamily C Member 3; previously known as MRP3) is overexpressed in PDAC cell lines and also in clinical samples. We demonstrate that ABCC3 expression is regulated by mutant p53 via miR-34 and that the transporter drives PDAC progression via transport of the bioactive lipid lysophosphatidylinositol (LPI). Disruption of ABCC3 function either by genetic knockdown reduces pancreatic cancer cell growth in vitro and in vivo. Mechanistically, we demonstrate that knockdown of ABCC3 reduce cell proliferation by inhibition of STAT3 and HIF1α signalling pathways, previously been shown to be key regulators of PDAC progression. Collectively, our results identify ABCC3 as a novel and promising target in PDAC therapy.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Carcinoma, Pancreatic Ductal/drug therapy, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Humans, Mice, Mice, Nude, Multidrug Resistance-Associated Proteins/antagonists & inhibitors, Pancreatic Neoplasms/drug therapy, Tumor Suppressor Protein p53/genetics, Xenograft Model Antitumor Assays
in
Advances in Biological Regulation
volume
73
article number
100634
pages
14 pages
publisher
Elsevier
external identifiers
  • scopus:85064920579
  • pmid:31053501
ISSN
2212-4926
DOI
10.1016/j.jbior.2019.04.004
language
English
LU publication?
no
additional info
Crown Copyright © 2019. Published by Elsevier Ltd. All rights reserved.
id
9eb61f0e-2397-41b8-a946-2c4a23716e73
date added to LUP
2023-05-22 10:41:02
date last changed
2024-04-19 21:55:52
@article{9eb61f0e-2397-41b8-a946-2c4a23716e73,
  abstract     = {{<p>Pancreatic Ductal Adenocarcinoma (PDAC) is a very aggressive disease, lacking effective therapeutic approaches and leaving PDAC patients with a poor prognosis. The life expectancy of PDAC patients has not experienced a significant change in the last few decades with a five-year survival rate of only 8%. To address this unmet need, novel pharmacological targets must be identified for clinical intervention. ATP Binding Cassette (ABC) transporters are frequently overexpressed in different cancer types and represent one of the major mechanisms responsible for chemoresistance. However, a more direct role for ABC transporters in tumorigenesis has not been widely investigated. Here, we show that ABCC3 (ABC Subfamily C Member 3; previously known as MRP3) is overexpressed in PDAC cell lines and also in clinical samples. We demonstrate that ABCC3 expression is regulated by mutant p53 via miR-34 and that the transporter drives PDAC progression via transport of the bioactive lipid lysophosphatidylinositol (LPI). Disruption of ABCC3 function either by genetic knockdown reduces pancreatic cancer cell growth in vitro and in vivo. Mechanistically, we demonstrate that knockdown of ABCC3 reduce cell proliferation by inhibition of STAT3 and HIF1α signalling pathways, previously been shown to be key regulators of PDAC progression. Collectively, our results identify ABCC3 as a novel and promising target in PDAC therapy.</p>}},
  author       = {{Adamska, Aleksandra and Ferro, Riccardo and Lattanzio, Rossano and Capone, Emily and Domenichini, Alice and Damiani, Verena and Chiorino, Giovanna and Akkaya, Begum Gokcen and Linton, Kenneth J and De Laurenzi, Vincenzo and Sala, Gianluca and Falasca, Marco}},
  issn         = {{2212-4926}},
  keywords     = {{Animals; Carcinoma, Pancreatic Ductal/drug therapy; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Humans; Mice; Mice, Nude; Multidrug Resistance-Associated Proteins/antagonists & inhibitors; Pancreatic Neoplasms/drug therapy; Tumor Suppressor Protein p53/genetics; Xenograft Model Antitumor Assays}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Advances in Biological Regulation}},
  title        = {{ABCC3 is a novel target for the treatment of pancreatic cancer}},
  url          = {{http://dx.doi.org/10.1016/j.jbior.2019.04.004}},
  doi          = {{10.1016/j.jbior.2019.04.004}},
  volume       = {{73}},
  year         = {{2019}},
}