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The pancreatic β-cell recognition of insulin secretagogues. VII. Binding and permeation of chloromercuribenzene-p-sulphonic acid in the plasma membrane of pancreatic β-cells

Hellman, Bo ; Lernmark, Åke LU orcid ; Sehlin, Janove ; Söderberg, Monica and Täljedal, Inge Bert (1973) In Archives of Biochemistry and Biophysics 158(1). p.435-441
Abstract

The uptake of chloromercuribenzene-p-sulphonic acid (CMBS) was studied in microdissected pancreatic islets of ob/ob-mice. After rapid initial binding, the uptake increased linearly with time, suggesting that CMBS diffused into the plasma membrane. The binding of CMBS was rapidly reversed on exposure to l-cysteine. Whereas glibenclamide had no effect, glucose and 4-acetamido-4′-isothiocyanostilbene-2,2′-disulphonic acid (SITS) inhibited diffusion without affecting the initial binding. SITS, but not glucose, also inhibited CMBS-induced insulin release. The results support the hypothesis that CMBS stimulates insulin release by reacting with thiol groups in the β-cell plasma membrane. These thiol groups may be located in an anion diffusion... (More)

The uptake of chloromercuribenzene-p-sulphonic acid (CMBS) was studied in microdissected pancreatic islets of ob/ob-mice. After rapid initial binding, the uptake increased linearly with time, suggesting that CMBS diffused into the plasma membrane. The binding of CMBS was rapidly reversed on exposure to l-cysteine. Whereas glibenclamide had no effect, glucose and 4-acetamido-4′-isothiocyanostilbene-2,2′-disulphonic acid (SITS) inhibited diffusion without affecting the initial binding. SITS, but not glucose, also inhibited CMBS-induced insulin release. The results support the hypothesis that CMBS stimulates insulin release by reacting with thiol groups in the β-cell plasma membrane. These thiol groups may be located in an anion diffusion channel, entrance to which is blocked by SITS and exit from which is inhibited by glucose. In comparison with erythrocytes, the β-cells contain a large number of superficial thiol groups, which may explain why these cells accumulate alloxan.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Archives of Biochemistry and Biophysics
volume
158
issue
1
pages
7 pages
publisher
Academic Press
external identifiers
  • scopus:0015694178
  • pmid:4199636
ISSN
0003-9861
DOI
10.1016/0003-9861(73)90640-1
language
English
LU publication?
no
id
9ec6f28d-df80-484a-a2cd-7521b30cd5e5
date added to LUP
2019-09-18 12:21:37
date last changed
2024-03-13 08:20:24
@article{9ec6f28d-df80-484a-a2cd-7521b30cd5e5,
  abstract     = {{<p>The uptake of chloromercuribenzene-p-sulphonic acid (CMBS) was studied in microdissected pancreatic islets of ob/ob-mice. After rapid initial binding, the uptake increased linearly with time, suggesting that CMBS diffused into the plasma membrane. The binding of CMBS was rapidly reversed on exposure to l-cysteine. Whereas glibenclamide had no effect, glucose and 4-acetamido-4′-isothiocyanostilbene-2,2′-disulphonic acid (SITS) inhibited diffusion without affecting the initial binding. SITS, but not glucose, also inhibited CMBS-induced insulin release. The results support the hypothesis that CMBS stimulates insulin release by reacting with thiol groups in the β-cell plasma membrane. These thiol groups may be located in an anion diffusion channel, entrance to which is blocked by SITS and exit from which is inhibited by glucose. In comparison with erythrocytes, the β-cells contain a large number of superficial thiol groups, which may explain why these cells accumulate alloxan.</p>}},
  author       = {{Hellman, Bo and Lernmark, Åke and Sehlin, Janove and Söderberg, Monica and Täljedal, Inge Bert}},
  issn         = {{0003-9861}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{1}},
  pages        = {{435--441}},
  publisher    = {{Academic Press}},
  series       = {{Archives of Biochemistry and Biophysics}},
  title        = {{The pancreatic β-cell recognition of insulin secretagogues. VII. Binding and permeation of chloromercuribenzene-p-sulphonic acid in the plasma membrane of pancreatic β-cells}},
  url          = {{http://dx.doi.org/10.1016/0003-9861(73)90640-1}},
  doi          = {{10.1016/0003-9861(73)90640-1}},
  volume       = {{158}},
  year         = {{1973}},
}