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Fast egg collection method greatly improves randomness of egg sampling in Drosophila melanogaster

Schou, Mads Fristrup LU (2013) In Fly 7(1). p.44-46
Abstract
When obtaining samples for population genetic studies, it is essential that the sampling is random. For Drosophila, one of the crucial steps in sampling experimental flies is the collection of eggs. Here an egg collection method is presented, which randomizes the eggs in a water column and diminishes environmental variance. This method was compared with a traditional egg collection method where eggs are collected directly from the medium. Within each method the observed and expected standard deviations of egg-to-adult viability were compared, whereby the difference in the randomness of the samples between the two methods was assessed. The method presented here was superior to the traditional method. Only 14% of the samples had a standard... (More)
When obtaining samples for population genetic studies, it is essential that the sampling is random. For Drosophila, one of the crucial steps in sampling experimental flies is the collection of eggs. Here an egg collection method is presented, which randomizes the eggs in a water column and diminishes environmental variance. This method was compared with a traditional egg collection method where eggs are collected directly from the medium. Within each method the observed and expected standard deviations of egg-to-adult viability were compared, whereby the difference in the randomness of the samples between the two methods was assessed. The method presented here was superior to the traditional method. Only 14% of the samples had a standard deviation higher than expected, as compared with 58% in the traditional method. To reduce bias in the estimation of the variance and the mean of a trait and to obtain a representative collection of genotypes, the method presented here is strongly recommended when collecting eggs from Drosophila. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Egg-to-adult viability, density control, random sampling, reliability, sampling error
in
Fly
volume
7
issue
1
pages
3 pages
publisher
Landes Bioscience
external identifiers
  • scopus:84873845324
ISSN
1933-6942
DOI
10.4161/fly.22758
language
English
LU publication?
no
id
9ed3e122-4939-4bf8-9497-69d2819de22d
date added to LUP
2017-12-18 09:44:03
date last changed
2018-05-29 11:17:04
@article{9ed3e122-4939-4bf8-9497-69d2819de22d,
  abstract     = {When obtaining samples for population genetic studies, it is essential that the sampling is random. For Drosophila, one of the crucial steps in sampling experimental flies is the collection of eggs. Here an egg collection method is presented, which randomizes the eggs in a water column and diminishes environmental variance. This method was compared with a traditional egg collection method where eggs are collected directly from the medium. Within each method the observed and expected standard deviations of egg-to-adult viability were compared, whereby the difference in the randomness of the samples between the two methods was assessed. The method presented here was superior to the traditional method. Only 14% of the samples had a standard deviation higher than expected, as compared with 58% in the traditional method. To reduce bias in the estimation of the variance and the mean of a trait and to obtain a representative collection of genotypes, the method presented here is strongly recommended when collecting eggs from Drosophila.},
  author       = {Schou, Mads Fristrup},
  issn         = {1933-6942},
  keyword      = {Egg-to-adult viability,density control,random sampling,reliability,sampling error},
  language     = {eng},
  number       = {1},
  pages        = {44--46},
  publisher    = {Landes Bioscience},
  series       = {Fly},
  title        = {Fast egg collection method greatly improves randomness of egg sampling in Drosophila melanogaster},
  url          = {http://dx.doi.org/10.4161/fly.22758},
  volume       = {7},
  year         = {2013},
}