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Short-term adaptation improves the fermentation performance of Saccharomyces cerevisiae in the presence of acetic acid at low pH.

Sanchez Nogue, Violeta LU ; Narayanan, Venkatachalam LU and Gorwa-Grauslund, Marie-Francoise LU (2013) In Applied Microbiology and Biotechnology 97(16). p.7517-7525
Abstract
The release of acetic acid due to deacetylation of the hemicellulose fraction during the treatment of lignocellulosic biomass contributes to the inhibitory character of the generated hydrolysates. In the present study, we identified a strain-independent adaptation protocol consisting of pre-cultivating the strain at pH 5.0 in the presence of at least 4 g L(-1) acetic acid that enabled aerobic growth and improved fermentation performance of Saccharomyces cerevisiae cells at low pH (3.7) and in the presence of inhibitory levels of acetic acid (6 g L(-1)). During anaerobic cultivation with adapted cells of strain TMB3500, the specific ethanol production rate was increased, reducing the fermentation time to 48 %.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Microbiology and Biotechnology
volume
97
issue
16
pages
7517 - 7525
publisher
Springer
external identifiers
  • wos:000322408100042
  • pmid:23872959
  • scopus:84881260879
  • pmid:23872959
ISSN
1432-0614
DOI
10.1007/s00253-013-5093-5
language
English
LU publication?
yes
id
9ee98f3a-2698-468d-9dbe-dd5de2f58d1a (old id 3955712)
date added to LUP
2016-04-01 10:35:19
date last changed
2020-01-12 04:29:47
@article{9ee98f3a-2698-468d-9dbe-dd5de2f58d1a,
  abstract     = {The release of acetic acid due to deacetylation of the hemicellulose fraction during the treatment of lignocellulosic biomass contributes to the inhibitory character of the generated hydrolysates. In the present study, we identified a strain-independent adaptation protocol consisting of pre-cultivating the strain at pH 5.0 in the presence of at least 4 g L(-1) acetic acid that enabled aerobic growth and improved fermentation performance of Saccharomyces cerevisiae cells at low pH (3.7) and in the presence of inhibitory levels of acetic acid (6 g L(-1)). During anaerobic cultivation with adapted cells of strain TMB3500, the specific ethanol production rate was increased, reducing the fermentation time to 48 %.},
  author       = {Sanchez Nogue, Violeta and Narayanan, Venkatachalam and Gorwa-Grauslund, Marie-Francoise},
  issn         = {1432-0614},
  language     = {eng},
  number       = {16},
  pages        = {7517--7525},
  publisher    = {Springer},
  series       = {Applied Microbiology and Biotechnology},
  title        = {Short-term adaptation improves the fermentation performance of Saccharomyces cerevisiae in the presence of acetic acid at low pH.},
  url          = {http://dx.doi.org/10.1007/s00253-013-5093-5},
  doi          = {10.1007/s00253-013-5093-5},
  volume       = {97},
  year         = {2013},
}