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Sag-rad : A Method for Single-Cell Population Genomics of Unicellular Eukaryotes

Gollnisch, Raphael LU ; Wallenius, Joel LU orcid ; Gribble, Kristin E ; Ahrén, Dag LU orcid and Rengefors, Karin LU (2023) In Molecular biology and evolution 40(5).
Abstract

Sequencing of reduced representation libraries enables genotyping of many individuals for population genomic studies. However, high amounts of DNA are required, and the method cannot be applied directly on single cells, preventing its use on most microbes. We developed and implemented the analysis of single amplified genomes followed by restriction-site-associated DNA sequencing to bypass labor-intensive culturing and to avoid culturing bias in population genomic studies of unicellular eukaryotes. This method thus opens the way for addressing important questions about the genetic diversity, gene flow, adaptation, dispersal, and biogeography of hitherto unexplored species.

Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Metagenomics, Eukaryota/genetics, Genomics/methods, Genome, Sequence Analysis, DNA/methods
in
Molecular biology and evolution
volume
40
issue
5
article number
msad095
pages
8 pages
publisher
Oxford University Press
external identifiers
  • scopus:85159776788
  • pmid:37079883
ISSN
0737-4038
DOI
10.1093/molbev/msad095
language
English
LU publication?
yes
additional info
© The Author(s) 2023. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution.
id
9f0394b4-11cc-4017-bb49-2e297ce74e08
date added to LUP
2023-07-10 15:31:32
date last changed
2024-04-19 23:18:45
@article{9f0394b4-11cc-4017-bb49-2e297ce74e08,
  abstract     = {{<p>Sequencing of reduced representation libraries enables genotyping of many individuals for population genomic studies. However, high amounts of DNA are required, and the method cannot be applied directly on single cells, preventing its use on most microbes. We developed and implemented the analysis of single amplified genomes followed by restriction-site-associated DNA sequencing to bypass labor-intensive culturing and to avoid culturing bias in population genomic studies of unicellular eukaryotes. This method thus opens the way for addressing important questions about the genetic diversity, gene flow, adaptation, dispersal, and biogeography of hitherto unexplored species.</p>}},
  author       = {{Gollnisch, Raphael and Wallenius, Joel and Gribble, Kristin E and Ahrén, Dag and Rengefors, Karin}},
  issn         = {{0737-4038}},
  keywords     = {{Metagenomics; Eukaryota/genetics; Genomics/methods; Genome; Sequence Analysis, DNA/methods}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{5}},
  publisher    = {{Oxford University Press}},
  series       = {{Molecular biology and evolution}},
  title        = {{Sag-rad : A Method for Single-Cell Population Genomics of Unicellular Eukaryotes}},
  url          = {{http://dx.doi.org/10.1093/molbev/msad095}},
  doi          = {{10.1093/molbev/msad095}},
  volume       = {{40}},
  year         = {{2023}},
}