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Capacitive Saccharide Sensor Based on Immobilized Phenylboronic Acid with Diol Specificity

Bergdahl, Gizem Ertürk LU ; Hedström, Martin LU and Mattiasson, Bo LU (2018) In Applied Biochemistry and Biotechnology
Abstract

A capacitive sensor for saccharide detection is described in this study. The detection is based on selective interaction between diols and aminophenylboronic acid (APBA) immobilized on a gold electrode. Glucose, fructose, and dextran (MW: 40 kDa) were tested with the system over wide concentration ranges (1.0 x 10−8 M - 1.0 x 10−3 M for glucose, 1.0 x 10−8 M - 1.0 x 10−2 M for fructose and 1.0 x 10−10 M - 1.0 x 10−5 M for dextran). The limits of detection (LODs) were 0.8 nM for glucose, 0.6 nM for fructose, and 13 pM for dextran. These data were comparable to the others reported previously. In order to demonstrate glycoprotein detection with the same sensor, human... (More)

A capacitive sensor for saccharide detection is described in this study. The detection is based on selective interaction between diols and aminophenylboronic acid (APBA) immobilized on a gold electrode. Glucose, fructose, and dextran (MW: 40 kDa) were tested with the system over wide concentration ranges (1.0 x 10−8 M - 1.0 x 10−3 M for glucose, 1.0 x 10−8 M - 1.0 x 10−2 M for fructose and 1.0 x 10−10 M - 1.0 x 10−5 M for dextran). The limits of detection (LODs) were 0.8 nM for glucose, 0.6 nM for fructose, and 13 pM for dextran. These data were comparable to the others reported previously. In order to demonstrate glycoprotein detection with the same sensor, human immunoglobulin G (IgG) as well as horseradish peroxidase were used as model analytes. The sensor responded to IgG in the concentration range of 1.0 x 10−13 M - 1.0 x 10−7 M with a LOD value of 16 fM. The performance of the assay of peroxidase was compared to a spectrophotometric assay by determining the enzymatic activity of a captured analyte. The results showed that the method might be useful for label-free, fast, and sensitive detection of saccharides as well as glycoproteins over a wide concentration range.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Aminophenylboronic acid, Capacitive sensor, Glycoprotein detection, Saccharide detection
in
Applied Biochemistry and Biotechnology
publisher
Humana Press
external identifiers
  • scopus:85055932573
ISSN
0273-2289
DOI
10.1007/s12010-018-2911-3
language
English
LU publication?
yes
id
9f128505-60c2-4223-b50d-d40bf90a1584
date added to LUP
2018-11-20 08:49:36
date last changed
2019-02-20 11:36:43
@article{9f128505-60c2-4223-b50d-d40bf90a1584,
  abstract     = {<p>A capacitive sensor for saccharide detection is described in this study. The detection is based on selective interaction between diols and aminophenylboronic acid (APBA) immobilized on a gold electrode. Glucose, fructose, and dextran (MW: 40 kDa) were tested with the system over wide concentration ranges (1.0 x 10<sup>−8</sup> M - 1.0 x 10<sup>−3</sup> M for glucose, 1.0 x 10<sup>−8</sup> M - 1.0 x 10<sup>−2</sup> M for fructose and 1.0 x 10<sup>−10</sup> M - 1.0 x 10<sup>−5</sup> M for dextran). The limits of detection (LODs) were 0.8 nM for glucose, 0.6 nM for fructose, and 13 pM for dextran. These data were comparable to the others reported previously. In order to demonstrate glycoprotein detection with the same sensor, human immunoglobulin G (IgG) as well as horseradish peroxidase were used as model analytes. The sensor responded to IgG in the concentration range of 1.0 x 10<sup>−13</sup> M - 1.0 x 10<sup>−7</sup> M with a LOD value of 16 fM. The performance of the assay of peroxidase was compared to a spectrophotometric assay by determining the enzymatic activity of a captured analyte. The results showed that the method might be useful for label-free, fast, and sensitive detection of saccharides as well as glycoproteins over a wide concentration range.</p>},
  author       = {Bergdahl, Gizem Ertürk and Hedström, Martin and Mattiasson, Bo},
  issn         = {0273-2289},
  keyword      = {Aminophenylboronic acid,Capacitive sensor,Glycoprotein detection,Saccharide detection},
  language     = {eng},
  month        = {10},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Capacitive Saccharide Sensor Based on Immobilized Phenylboronic Acid with Diol Specificity},
  url          = {http://dx.doi.org/10.1007/s12010-018-2911-3},
  year         = {2018},
}