Poly(hydroxyethyl methacrylate)-based composite cryogel with embedded macroporous cellulose beads for the separation of human serum immunoglobulin and albumin
(2013) In Journal of Separation Science 36(24). p.3813-3820- Abstract
- A novel super-macroporous monolithic composite cryogel was prepared by embedding macroporous cellulose beads into poly(hydroxyethyl methacrylate) cryogel. The cellulose beads were fabricated by using a microchannel liquid-flow focusing and cryopolymerization method, while the composite cryogel was prepared by cryogenic radical polymerization of the hydroxyethyl methacrylate monomer with poly(ethylene glycol) diacrylate as cross-linker together with the cellulose beads. After graft polymerization with (vinylbenzyl)trimethylammonium chloride, the composite cryogel was applied to separate immunoglobulin-G and albumin from human serum. Immunoglobulin-G with a mean purity of 83.2% and albumin with a purity of 98% were obtained, indicating the... (More)
- A novel super-macroporous monolithic composite cryogel was prepared by embedding macroporous cellulose beads into poly(hydroxyethyl methacrylate) cryogel. The cellulose beads were fabricated by using a microchannel liquid-flow focusing and cryopolymerization method, while the composite cryogel was prepared by cryogenic radical polymerization of the hydroxyethyl methacrylate monomer with poly(ethylene glycol) diacrylate as cross-linker together with the cellulose beads. After graft polymerization with (vinylbenzyl)trimethylammonium chloride, the composite cryogel was applied to separate immunoglobulin-G and albumin from human serum. Immunoglobulin-G with a mean purity of 83.2% and albumin with a purity of 98% were obtained, indicating the composite cryogel as a promising chromatographic medium in bioseparation for the isolation of important bioactive proteins like immunoglobulins and albumins. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4269100
- author
- Ye, Jialei ; Yun, Junxian ; Lin, Dong-Qiang ; Xu, Linhong ; Kirsebom, Harald LU ; Shen, Shaochuan ; Yang, Gensheng ; Yao, Kejian ; Guan, Yi-Xin and Yao, Shan-Jing
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bioseparation, Cellulose beads, Cryogels, Immunoglobulin-G, Ion-exchange, adsorption
- in
- Journal of Separation Science
- volume
- 36
- issue
- 24
- pages
- 3813 - 3820
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000328138200001
- scopus:84890461139
- pmid:24151195
- ISSN
- 1615-9314
- DOI
- 10.1002/jssc.201300911
- language
- English
- LU publication?
- yes
- id
- 9f931a1f-490a-4244-a7a9-78d6c7791e85 (old id 4269100)
- date added to LUP
- 2016-04-01 09:47:26
- date last changed
- 2022-03-11 23:06:56
@article{9f931a1f-490a-4244-a7a9-78d6c7791e85,
abstract = {{A novel super-macroporous monolithic composite cryogel was prepared by embedding macroporous cellulose beads into poly(hydroxyethyl methacrylate) cryogel. The cellulose beads were fabricated by using a microchannel liquid-flow focusing and cryopolymerization method, while the composite cryogel was prepared by cryogenic radical polymerization of the hydroxyethyl methacrylate monomer with poly(ethylene glycol) diacrylate as cross-linker together with the cellulose beads. After graft polymerization with (vinylbenzyl)trimethylammonium chloride, the composite cryogel was applied to separate immunoglobulin-G and albumin from human serum. Immunoglobulin-G with a mean purity of 83.2% and albumin with a purity of 98% were obtained, indicating the composite cryogel as a promising chromatographic medium in bioseparation for the isolation of important bioactive proteins like immunoglobulins and albumins.}},
author = {{Ye, Jialei and Yun, Junxian and Lin, Dong-Qiang and Xu, Linhong and Kirsebom, Harald and Shen, Shaochuan and Yang, Gensheng and Yao, Kejian and Guan, Yi-Xin and Yao, Shan-Jing}},
issn = {{1615-9314}},
keywords = {{Bioseparation; Cellulose beads; Cryogels; Immunoglobulin-G; Ion-exchange; adsorption}},
language = {{eng}},
number = {{24}},
pages = {{3813--3820}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Separation Science}},
title = {{Poly(hydroxyethyl methacrylate)-based composite cryogel with embedded macroporous cellulose beads for the separation of human serum immunoglobulin and albumin}},
url = {{http://dx.doi.org/10.1002/jssc.201300911}},
doi = {{10.1002/jssc.201300911}},
volume = {{36}},
year = {{2013}},
}