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Retinoic acid promotes differentiation of WiT49- but not of CCG99-11 Wilms tumour cells

Jansson, Caroline LU and Mengelbier, Linda Holmquist LU (2023) In Cancer Reports 6(6).
Abstract

Background: Most children with Wilms tumour are successfully treated with multidrug chemotherapy and surgery. These treatments cause severe side effects for the patients, an issue that needs to be addressed by exploring other treatment options with less or no side effects. One option is to complement current therapies with agents that could potentially induce tumour cell differentiation, for example retinoic acid (RA). Aims: To facilitate quick assessment of an agent's effect on Wilms tumour differentiation by a rapid in vitro model system. Methods and Results: Here WiT49 and CCG99-11 Wilms tumour cells were treated with 10 μM RA for 72 h or 9 days. Cultured cells were scraped off from Petri dishes, pelleted and embedded in paraffin in... (More)

Background: Most children with Wilms tumour are successfully treated with multidrug chemotherapy and surgery. These treatments cause severe side effects for the patients, an issue that needs to be addressed by exploring other treatment options with less or no side effects. One option is to complement current therapies with agents that could potentially induce tumour cell differentiation, for example retinoic acid (RA). Aims: To facilitate quick assessment of an agent's effect on Wilms tumour differentiation by a rapid in vitro model system. Methods and Results: Here WiT49 and CCG99-11 Wilms tumour cells were treated with 10 μM RA for 72 h or 9 days. Cultured cells were scraped off from Petri dishes, pelleted and embedded in paraffin in the same way as clinical tumour specimens are preserved. Cell morphology and differentiation were evaluated by analyses of haematoxylin eosin (H&E) and immunohistochemical stainings. Based on H&E, WT1 and CKAE1/3 stainings, RA treatment induced further epithelial differentiation of WiT49 cells, whereas there was no sign of induced maturation in CCG99-11 cells. Ki67 staining showed that RA inhibited cell proliferation in both cell lines. Conclusions: Our study shows that in vitro culturing of WiT49 and CCG99-11 cells, followed by pelleting and paraffin embedding of cell pellets, could aid in a quick evaluation of potential differentiating agents against Wilms tumour. In addition, our results strengthen previous results that retinoic acid could be a potential complement to regular Wilms tumour treatment.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CCG99-11, differentiation, model system, retinoic acid, Wilms tumour, WiT49
in
Cancer Reports
volume
6
issue
6
article number
e1819
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:37186071
  • scopus:85153608495
ISSN
2573-8348
DOI
10.1002/cnr2.1819
language
English
LU publication?
yes
id
9fbb0a65-0f60-4344-8718-a2bfc7fb46da
date added to LUP
2023-07-14 11:45:24
date last changed
2024-06-29 05:48:48
@article{9fbb0a65-0f60-4344-8718-a2bfc7fb46da,
  abstract     = {{<p>Background: Most children with Wilms tumour are successfully treated with multidrug chemotherapy and surgery. These treatments cause severe side effects for the patients, an issue that needs to be addressed by exploring other treatment options with less or no side effects. One option is to complement current therapies with agents that could potentially induce tumour cell differentiation, for example retinoic acid (RA). Aims: To facilitate quick assessment of an agent's effect on Wilms tumour differentiation by a rapid in vitro model system. Methods and Results: Here WiT49 and CCG99-11 Wilms tumour cells were treated with 10 μM RA for 72 h or 9 days. Cultured cells were scraped off from Petri dishes, pelleted and embedded in paraffin in the same way as clinical tumour specimens are preserved. Cell morphology and differentiation were evaluated by analyses of haematoxylin eosin (H&amp;E) and immunohistochemical stainings. Based on H&amp;E, WT1 and CKAE1/3 stainings, RA treatment induced further epithelial differentiation of WiT49 cells, whereas there was no sign of induced maturation in CCG99-11 cells. Ki67 staining showed that RA inhibited cell proliferation in both cell lines. Conclusions: Our study shows that in vitro culturing of WiT49 and CCG99-11 cells, followed by pelleting and paraffin embedding of cell pellets, could aid in a quick evaluation of potential differentiating agents against Wilms tumour. In addition, our results strengthen previous results that retinoic acid could be a potential complement to regular Wilms tumour treatment.</p>}},
  author       = {{Jansson, Caroline and Mengelbier, Linda Holmquist}},
  issn         = {{2573-8348}},
  keywords     = {{CCG99-11; differentiation; model system; retinoic acid; Wilms tumour; WiT49}},
  language     = {{eng}},
  number       = {{6}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Cancer Reports}},
  title        = {{Retinoic acid promotes differentiation of WiT49- but not of CCG99-11 Wilms tumour cells}},
  url          = {{http://dx.doi.org/10.1002/cnr2.1819}},
  doi          = {{10.1002/cnr2.1819}},
  volume       = {{6}},
  year         = {{2023}},
}