Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Blinking statistics of a molecular beacon triggered by end-denaturation of DNA

Ambjörnsson, Tobias LU and Metzler, Ralf (2005) In Journal of Physics: Condensed Matter 17(49). p.4305-4316
Abstract
We use a master equation approach based on the Poland–Scheraga free energy for DNA denaturation to investigate the (un)zipping dynamics of a denaturation wedge in a stretch of DNA that is clamped at one end. In particular, we quantify the blinking dynamics of a fluorophore–quencher pair mounted within the denaturation wedge. We also study the behavioural changes in the presence of proteins that selectively bind to single-stranded DNA. We show that such a set-up could be well suited as an easy-to-implement nanodevice for sensing environmental conditions in small volumes.
Please use this url to cite or link to this publication:
author
and
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Physics: Condensed Matter
volume
17
issue
49
pages
4305 - 4316
publisher
IOP Publishing
external identifiers
  • scopus:28544451982
ISSN
0953-8984
DOI
10.1088/0953-8984/17/49/022
language
English
LU publication?
no
id
9fec72ce-cd88-41ae-ba5e-f94297f6093c
date added to LUP
2019-05-03 11:43:32
date last changed
2022-03-31 17:19:48
@article{9fec72ce-cd88-41ae-ba5e-f94297f6093c,
  abstract     = {{We use a master equation approach based on the Poland–Scheraga free energy for DNA denaturation to investigate the (un)zipping dynamics of a denaturation wedge in a stretch of DNA that is clamped at one end. In particular, we quantify the blinking dynamics of a fluorophore–quencher pair mounted within the denaturation wedge. We also study the behavioural changes in the presence of proteins that selectively bind to single-stranded DNA. We show that such a set-up could be well suited as an easy-to-implement nanodevice for sensing environmental conditions in small volumes.}},
  author       = {{Ambjörnsson, Tobias and Metzler, Ralf}},
  issn         = {{0953-8984}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{49}},
  pages        = {{4305--4316}},
  publisher    = {{IOP Publishing}},
  series       = {{Journal of Physics: Condensed Matter}},
  title        = {{Blinking statistics of a molecular beacon triggered by end-denaturation of DNA}},
  url          = {{http://dx.doi.org/10.1088/0953-8984/17/49/022}},
  doi          = {{10.1088/0953-8984/17/49/022}},
  volume       = {{17}},
  year         = {{2005}},
}