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Towards virtual histology with X-ray grating interferometry

Polikarpov, M. ; Vila-Comamala, J. ; Wang, Z. ; Pereira, A. ; Van gogh, S. ; Gasser, C. ; Jefimovs, K. ; Romano, L. ; Varga, Z. and Lång, K. LU , et al. (2023) In Scientific Reports 13. p.1-11
Abstract
Breast cancer is the most common type of cancer worldwide. Diagnosing breast cancer relies on clinical examination, imaging and biopsy. A core-needle biopsy enables a morphological and biochemical characterization of the cancer and is considered the gold standard for breast cancer diagnosis. A histopathological examination uses high-resolution microscopes with outstanding contrast in the 2D plane, but the spatial resolution in the third, Z-direction, is reduced. In the present paper, we propose two high-resolution table-top systems for phase-contrast X-ray tomography of soft-tissue samples. The first system implements a classical Talbot–Lau interferometer and allows to perform ex-vivo imaging of human breast samples with a voxel size of... (More)
Breast cancer is the most common type of cancer worldwide. Diagnosing breast cancer relies on clinical examination, imaging and biopsy. A core-needle biopsy enables a morphological and biochemical characterization of the cancer and is considered the gold standard for breast cancer diagnosis. A histopathological examination uses high-resolution microscopes with outstanding contrast in the 2D plane, but the spatial resolution in the third, Z-direction, is reduced. In the present paper, we propose two high-resolution table-top systems for phase-contrast X-ray tomography of soft-tissue samples. The first system implements a classical Talbot–Lau interferometer and allows to perform ex-vivo imaging of human breast samples with a voxel size of 5.57 μm. The second system with a comparable voxel size relies on a Sigray MAAST X-ray source with structured anode. For the first time, we demonstrate the applicability of the latter to perform X-ray imaging of human breast specimens with ductal carcinoma in-situ. We assessed image quality of both setups and compared it to histology. We showed that both setups made it possible to target internal features of breast specimens with better resolution and contrast than previously achieved, demonstrating that grating-based phase-contrast X-ray CT could be a complementary tool for clinical histopathology. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
13
article number
9049
pages
1 - 11
publisher
Nature Publishing Group
external identifiers
  • scopus:85160893715
  • pmid:37270642
ISSN
2045-2322
DOI
10.1038/s41598-023-35854-6
language
English
LU publication?
yes
id
9ff5117f-7fd9-4345-b9ad-56fd7278d42d
date added to LUP
2023-06-25 16:00:46
date last changed
2023-06-27 03:00:03
@article{9ff5117f-7fd9-4345-b9ad-56fd7278d42d,
  abstract     = {{Breast cancer is the most common type of cancer worldwide. Diagnosing breast cancer relies on clinical examination, imaging and biopsy. A core-needle biopsy enables a morphological and biochemical characterization of the cancer and is considered the gold standard for breast cancer diagnosis. A histopathological examination uses high-resolution microscopes with outstanding contrast in the 2D plane, but the spatial resolution in the third, Z-direction, is reduced. In the present paper, we propose two high-resolution table-top systems for phase-contrast X-ray tomography of soft-tissue samples. The first system implements a classical Talbot–Lau interferometer and allows to perform ex-vivo imaging of human breast samples with a voxel size of 5.57 μm. The second system with a comparable voxel size relies on a Sigray MAAST X-ray source with structured anode. For the first time, we demonstrate the applicability of the latter to perform X-ray imaging of human breast specimens with ductal carcinoma in-situ. We assessed image quality of both setups and compared it to histology. We showed that both setups made it possible to target internal features of breast specimens with better resolution and contrast than previously achieved, demonstrating that grating-based phase-contrast X-ray CT could be a complementary tool for clinical histopathology.}},
  author       = {{Polikarpov, M. and Vila-Comamala, J. and Wang, Z. and Pereira, A. and Van gogh, S. and Gasser, C. and Jefimovs, K. and Romano, L. and Varga, Z. and Lång, K. and Schmeltz, M. and Tessarini, S. and Rawlik, M. and Jermann, E. and Lewis, S. and Yun, W. and Stampanoni, M.}},
  issn         = {{2045-2322}},
  language     = {{eng}},
  month        = {{06}},
  pages        = {{1--11}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{Towards virtual histology with X-ray grating interferometry}},
  url          = {{http://dx.doi.org/10.1038/s41598-023-35854-6}},
  doi          = {{10.1038/s41598-023-35854-6}},
  volume       = {{13}},
  year         = {{2023}},
}