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Transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms

Falås, Per LU ; Jewell, Kevin S.; Hermes, Nina; Wick, Arne; Ternes, Thomas A.; Joss, Adriano and Nielsen, Jeppe Lund (2018) In Water Research 141. p.405-416
Abstract

A tiered process was developed to assess the transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms from two municipal wastewater treatment plants. At the first tier, primary transformation of ibuprofen, naproxen, diclofenac, and mecoprop by carrier-attached microorganisms was shown by the dissipation of the target compounds and the formation of five transformation products using LC-tandem MS. At the second tier, the microbial cleavage of the four organic micropollutants was confirmed with 14C-labeled micropollutants through liquid scintillation counting of the 14CO2 formed. At the third tier, microautoradiography coupled with fluorescence in... (More)

A tiered process was developed to assess the transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms from two municipal wastewater treatment plants. At the first tier, primary transformation of ibuprofen, naproxen, diclofenac, and mecoprop by carrier-attached microorganisms was shown by the dissipation of the target compounds and the formation of five transformation products using LC-tandem MS. At the second tier, the microbial cleavage of the four organic micropollutants was confirmed with 14C-labeled micropollutants through liquid scintillation counting of the 14CO2 formed. At the third tier, microautoradiography coupled with fluorescence in situ hybridization (MAR-FISH) was used to screen carrier-attached microorganisms for uptake of the four radiolabeled micropollutants. Results from the MAR-FISH screening indicated that only a small fraction of the microbial community (≤1‰) was involved in the uptake of the radiolabeled micropollutants and that the responsible microorganisms differed between the compounds. At the fourth tier, the microbial community structure of the carrier-attached biofilms was analyzed by 16S rRNA gene amplicon sequencing. The sequencing results showed that the MAR-FISH screening targeted ∼80% of the microbial community and that several taxonomic families within the FISH-probed populations with MAR-positive signals (i.e. Firmicutes, Gammaproteobacteria, and Deltaproteobacteria) were present in both biofilms. From the broader perspective of organic micropollutant removal in biological wastewater treatment, the MAR-FISH results of this study indicate a high degree of microbial substrate specialization that could explain differences in transformation rates and patterns between micropollutants and microbial communities.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biological wastewater treatment, Microautoradiography, Micropollutants, Moving bed biofilm reactors, Transformation
in
Water Research
volume
141
pages
12 pages
publisher
Elsevier
external identifiers
  • scopus:85049331293
ISSN
0043-1354
DOI
10.1016/j.watres.2018.03.040
language
English
LU publication?
yes
id
9ff5bee5-d756-4a83-997a-c4cb8f5f0277
date added to LUP
2018-07-12 13:26:46
date last changed
2018-07-13 03:00:04
@article{9ff5bee5-d756-4a83-997a-c4cb8f5f0277,
  abstract     = {<p>A tiered process was developed to assess the transformation, CO<sub>2</sub> formation and uptake of four organic micropollutants by carrier-attached microorganisms from two municipal wastewater treatment plants. At the first tier, primary transformation of ibuprofen, naproxen, diclofenac, and mecoprop by carrier-attached microorganisms was shown by the dissipation of the target compounds and the formation of five transformation products using LC-tandem MS. At the second tier, the microbial cleavage of the four organic micropollutants was confirmed with <sup>14</sup>C-labeled micropollutants through liquid scintillation counting of the <sup>14</sup>CO<sub>2</sub> formed. At the third tier, microautoradiography coupled with fluorescence in situ hybridization (MAR-FISH) was used to screen carrier-attached microorganisms for uptake of the four radiolabeled micropollutants. Results from the MAR-FISH screening indicated that only a small fraction of the microbial community (≤1‰) was involved in the uptake of the radiolabeled micropollutants and that the responsible microorganisms differed between the compounds. At the fourth tier, the microbial community structure of the carrier-attached biofilms was analyzed by 16S rRNA gene amplicon sequencing. The sequencing results showed that the MAR-FISH screening targeted ∼80% of the microbial community and that several taxonomic families within the FISH-probed populations with MAR-positive signals (i.e. Firmicutes, Gammaproteobacteria, and Deltaproteobacteria) were present in both biofilms. From the broader perspective of organic micropollutant removal in biological wastewater treatment, the MAR-FISH results of this study indicate a high degree of microbial substrate specialization that could explain differences in transformation rates and patterns between micropollutants and microbial communities.</p>},
  author       = {Falås, Per and Jewell, Kevin S. and Hermes, Nina and Wick, Arne and Ternes, Thomas A. and Joss, Adriano and Nielsen, Jeppe Lund},
  issn         = {0043-1354},
  keyword      = {Biological wastewater treatment,Microautoradiography,Micropollutants,Moving bed biofilm reactors,Transformation},
  language     = {eng},
  month        = {09},
  pages        = {405--416},
  publisher    = {Elsevier},
  series       = {Water Research},
  title        = {Transformation, CO<sub>2</sub> formation and uptake of four organic micropollutants by carrier-attached microorganisms},
  url          = {http://dx.doi.org/10.1016/j.watres.2018.03.040},
  volume       = {141},
  year         = {2018},
}