Transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms
(2018) In Water Research 141. p.405-416- Abstract
A tiered process was developed to assess the transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms from two municipal wastewater treatment plants. At the first tier, primary transformation of ibuprofen, naproxen, diclofenac, and mecoprop by carrier-attached microorganisms was shown by the dissipation of the target compounds and the formation of five transformation products using LC-tandem MS. At the second tier, the microbial cleavage of the four organic micropollutants was confirmed with 14C-labeled micropollutants through liquid scintillation counting of the 14CO2 formed. At the third tier, microautoradiography coupled with fluorescence in... (More)
A tiered process was developed to assess the transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms from two municipal wastewater treatment plants. At the first tier, primary transformation of ibuprofen, naproxen, diclofenac, and mecoprop by carrier-attached microorganisms was shown by the dissipation of the target compounds and the formation of five transformation products using LC-tandem MS. At the second tier, the microbial cleavage of the four organic micropollutants was confirmed with 14C-labeled micropollutants through liquid scintillation counting of the 14CO2 formed. At the third tier, microautoradiography coupled with fluorescence in situ hybridization (MAR-FISH) was used to screen carrier-attached microorganisms for uptake of the four radiolabeled micropollutants. Results from the MAR-FISH screening indicated that only a small fraction of the microbial community (≤1‰) was involved in the uptake of the radiolabeled micropollutants and that the responsible microorganisms differed between the compounds. At the fourth tier, the microbial community structure of the carrier-attached biofilms was analyzed by 16S rRNA gene amplicon sequencing. The sequencing results showed that the MAR-FISH screening targeted ∼80% of the microbial community and that several taxonomic families within the FISH-probed populations with MAR-positive signals (i.e. Firmicutes, Gammaproteobacteria, and Deltaproteobacteria) were present in both biofilms. From the broader perspective of organic micropollutant removal in biological wastewater treatment, the MAR-FISH results of this study indicate a high degree of microbial substrate specialization that could explain differences in transformation rates and patterns between micropollutants and microbial communities.
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- author
- Falås, Per LU ; Jewell, Kevin S. ; Hermes, Nina ; Wick, Arne ; Ternes, Thomas A. ; Joss, Adriano and Nielsen, Jeppe Lund
- organization
- publishing date
- 2018-09-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biological wastewater treatment, Microautoradiography, Micropollutants, Moving bed biofilm reactors, Transformation
- in
- Water Research
- volume
- 141
- pages
- 12 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:29859473
- scopus:85049331293
- ISSN
- 0043-1354
- DOI
- 10.1016/j.watres.2018.03.040
- language
- English
- LU publication?
- yes
- id
- 9ff5bee5-d756-4a83-997a-c4cb8f5f0277
- date added to LUP
- 2018-07-12 13:26:46
- date last changed
- 2020-05-26 04:50:28
@article{9ff5bee5-d756-4a83-997a-c4cb8f5f0277,
abstract = {<p>A tiered process was developed to assess the transformation, CO<sub>2</sub> formation and uptake of four organic micropollutants by carrier-attached microorganisms from two municipal wastewater treatment plants. At the first tier, primary transformation of ibuprofen, naproxen, diclofenac, and mecoprop by carrier-attached microorganisms was shown by the dissipation of the target compounds and the formation of five transformation products using LC-tandem MS. At the second tier, the microbial cleavage of the four organic micropollutants was confirmed with <sup>14</sup>C-labeled micropollutants through liquid scintillation counting of the <sup>14</sup>CO<sub>2</sub> formed. At the third tier, microautoradiography coupled with fluorescence in situ hybridization (MAR-FISH) was used to screen carrier-attached microorganisms for uptake of the four radiolabeled micropollutants. Results from the MAR-FISH screening indicated that only a small fraction of the microbial community (≤1‰) was involved in the uptake of the radiolabeled micropollutants and that the responsible microorganisms differed between the compounds. At the fourth tier, the microbial community structure of the carrier-attached biofilms was analyzed by 16S rRNA gene amplicon sequencing. The sequencing results showed that the MAR-FISH screening targeted ∼80% of the microbial community and that several taxonomic families within the FISH-probed populations with MAR-positive signals (i.e. Firmicutes, Gammaproteobacteria, and Deltaproteobacteria) were present in both biofilms. From the broader perspective of organic micropollutant removal in biological wastewater treatment, the MAR-FISH results of this study indicate a high degree of microbial substrate specialization that could explain differences in transformation rates and patterns between micropollutants and microbial communities.</p>},
author = {Falås, Per and Jewell, Kevin S. and Hermes, Nina and Wick, Arne and Ternes, Thomas A. and Joss, Adriano and Nielsen, Jeppe Lund},
issn = {0043-1354},
language = {eng},
month = {09},
pages = {405--416},
publisher = {Elsevier},
series = {Water Research},
title = {Transformation, CO<sub>2</sub> formation and uptake of four organic micropollutants by carrier-attached microorganisms},
url = {http://dx.doi.org/10.1016/j.watres.2018.03.040},
doi = {10.1016/j.watres.2018.03.040},
volume = {141},
year = {2018},
}