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High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation

Laine, Romain F ; Heil, Hannah S LU orcid ; Coelho, Simao ; Nixon-Abell, Jonathon ; Jimenez, Angélique ; Wiesner, Theresa ; Martínez, Damián ; Galgani, Tommaso ; Régnier, Louise and Stubb, Aki , et al. (2023) In Nature Methods 20(12). p.1949-1956
Abstract

Live-cell super-resolution microscopy enables the imaging of biological structure dynamics below the diffraction limit. Here we present enhanced super-resolution radial fluctuations (eSRRF), substantially improving image fidelity and resolution compared to the original SRRF method. eSRRF incorporates automated parameter optimization based on the data itself, giving insight into the trade-off between resolution and fidelity. We demonstrate eSRRF across a range of imaging modalities and biological systems. Notably, we extend eSRRF to three dimensions by combining it with multifocus microscopy. This realizes live-cell volumetric super-resolution imaging with an acquisition speed of ~1 volume per second. eSRRF provides an accessible... (More)

Live-cell super-resolution microscopy enables the imaging of biological structure dynamics below the diffraction limit. Here we present enhanced super-resolution radial fluctuations (eSRRF), substantially improving image fidelity and resolution compared to the original SRRF method. eSRRF incorporates automated parameter optimization based on the data itself, giving insight into the trade-off between resolution and fidelity. We demonstrate eSRRF across a range of imaging modalities and biological systems. Notably, we extend eSRRF to three dimensions by combining it with multifocus microscopy. This realizes live-cell volumetric super-resolution imaging with an acquisition speed of ~1 volume per second. eSRRF provides an accessible super-resolution approach, maximizing information extraction across varied experimental conditions while minimizing artifacts. Its optimal parameter prediction strategy is generalizable, moving toward unbiased and optimized analyses in super-resolution microscopy.

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publishing date
type
Contribution to journal
publication status
published
keywords
Microscopy, Fluorescence/methods, Artifacts
in
Nature Methods
volume
20
issue
12
pages
1949 - 1956
publisher
Nature Publishing Group
external identifiers
  • pmid:37957430
  • scopus:85176404122
ISSN
1548-7105
DOI
10.1038/s41592-023-02057-w
language
English
LU publication?
no
additional info
© 2023. The Author(s).
id
a00aa4b2-a6b6-434b-bbe9-a762982ff861
date added to LUP
2025-04-07 16:06:02
date last changed
2025-08-26 15:15:43
@article{a00aa4b2-a6b6-434b-bbe9-a762982ff861,
  abstract     = {{<p>Live-cell super-resolution microscopy enables the imaging of biological structure dynamics below the diffraction limit. Here we present enhanced super-resolution radial fluctuations (eSRRF), substantially improving image fidelity and resolution compared to the original SRRF method. eSRRF incorporates automated parameter optimization based on the data itself, giving insight into the trade-off between resolution and fidelity. We demonstrate eSRRF across a range of imaging modalities and biological systems. Notably, we extend eSRRF to three dimensions by combining it with multifocus microscopy. This realizes live-cell volumetric super-resolution imaging with an acquisition speed of ~1 volume per second. eSRRF provides an accessible super-resolution approach, maximizing information extraction across varied experimental conditions while minimizing artifacts. Its optimal parameter prediction strategy is generalizable, moving toward unbiased and optimized analyses in super-resolution microscopy.</p>}},
  author       = {{Laine, Romain F and Heil, Hannah S and Coelho, Simao and Nixon-Abell, Jonathon and Jimenez, Angélique and Wiesner, Theresa and Martínez, Damián and Galgani, Tommaso and Régnier, Louise and Stubb, Aki and Follain, Gautier and Webster, Samantha and Goyette, Jesse and Dauphin, Aurelien and Salles, Audrey and Culley, Siân and Jacquemet, Guillaume and Hajj, Bassam and Leterrier, Christophe and Henriques, Ricardo}},
  issn         = {{1548-7105}},
  keywords     = {{Microscopy, Fluorescence/methods; Artifacts}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{1949--1956}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature Methods}},
  title        = {{High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation}},
  url          = {{http://dx.doi.org/10.1038/s41592-023-02057-w}},
  doi          = {{10.1038/s41592-023-02057-w}},
  volume       = {{20}},
  year         = {{2023}},
}