Species-specific autoantibodies in type 1 diabetes
(1999) In Journal of Clinical Endocrinology and Metabolism 84(2). p.643-648- Abstract
GAD65 autoantibodies (GAD65Ab) are important markers for type 1 (insulin-dependent) diabetes mellitus. Although most patients have GAD65Ab at the time of clinical diagnosis, there are also GAD65Ab-positive individuals in the population at low risk of developing type 1 diabetes. The aim of this study was to test the hypothesis that the GAD65Ab reactivity to GAD65 cloned from human, mouse, and rat in newly diagnosed type 1 diabetic patients differ from antibody-positive healthy individuals. Sera from 254 new-onset 0- to 34- yr-old type 1 diabetic patients and 270 controls were assayed for their reactivity to human, mouse, and rat GAD65. Among the type 1 diabetic patients there was a significant better binding of human GAD65 compared to... (More)
GAD65 autoantibodies (GAD65Ab) are important markers for type 1 (insulin-dependent) diabetes mellitus. Although most patients have GAD65Ab at the time of clinical diagnosis, there are also GAD65Ab-positive individuals in the population at low risk of developing type 1 diabetes. The aim of this study was to test the hypothesis that the GAD65Ab reactivity to GAD65 cloned from human, mouse, and rat in newly diagnosed type 1 diabetic patients differ from antibody-positive healthy individuals. Sera from 254 new-onset 0- to 34- yr-old type 1 diabetic patients and 270 controls were assayed for their reactivity to human, mouse, and rat GAD65. Among the type 1 diabetic patients there was a significant better binding of human GAD65 compared to either mouse (P = 0.03) or rat GAD65 (P = 0.0005). The preference for human GAD65 increased with increasing age at onset (P = 0.0002). This differentiation was not observed in 88 GAD65Ab-positive control subjects. Our data indicate that recognition of epitopes by GAD65Ab in type 1 diabetes is different from that in nontype 1 diabetes, GAD65Ab-positive individuals.
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- author
- Hampe, C. S. ; Örtqvist, E. ; Rolandsson, O. ; Landin-Olsson, M. LU ; Törn, C. LU ; Ågren, Å ; Persson, B. ; Schranz, D. B. and Lernmark, Å LU
- publishing date
- 1999-02-17
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Clinical Endocrinology and Metabolism
- volume
- 84
- issue
- 2
- pages
- 643 - 648
- publisher
- Oxford University Press
- external identifiers
-
- pmid:10022431
- scopus:0033018809
- ISSN
- 0021-972X
- DOI
- 10.1210/jcem.84.2.5503
- language
- English
- LU publication?
- no
- id
- a01b1220-c706-40c1-91a0-c0d069d6990f
- date added to LUP
- 2019-06-30 23:29:05
- date last changed
- 2024-04-16 15:15:19
@article{a01b1220-c706-40c1-91a0-c0d069d6990f, abstract = {{<p>GAD65 autoantibodies (GAD65Ab) are important markers for type 1 (insulin-dependent) diabetes mellitus. Although most patients have GAD65Ab at the time of clinical diagnosis, there are also GAD65Ab-positive individuals in the population at low risk of developing type 1 diabetes. The aim of this study was to test the hypothesis that the GAD65Ab reactivity to GAD65 cloned from human, mouse, and rat in newly diagnosed type 1 diabetic patients differ from antibody-positive healthy individuals. Sera from 254 new-onset 0- to 34- yr-old type 1 diabetic patients and 270 controls were assayed for their reactivity to human, mouse, and rat GAD65. Among the type 1 diabetic patients there was a significant better binding of human GAD65 compared to either mouse (P = 0.03) or rat GAD65 (P = 0.0005). The preference for human GAD65 increased with increasing age at onset (P = 0.0002). This differentiation was not observed in 88 GAD65Ab-positive control subjects. Our data indicate that recognition of epitopes by GAD65Ab in type 1 diabetes is different from that in nontype 1 diabetes, GAD65Ab-positive individuals.</p>}}, author = {{Hampe, C. S. and Örtqvist, E. and Rolandsson, O. and Landin-Olsson, M. and Törn, C. and Ågren, Å and Persson, B. and Schranz, D. B. and Lernmark, Å}}, issn = {{0021-972X}}, language = {{eng}}, month = {{02}}, number = {{2}}, pages = {{643--648}}, publisher = {{Oxford University Press}}, series = {{Journal of Clinical Endocrinology and Metabolism}}, title = {{Species-specific autoantibodies in type 1 diabetes}}, url = {{http://dx.doi.org/10.1210/jcem.84.2.5503}}, doi = {{10.1210/jcem.84.2.5503}}, volume = {{84}}, year = {{1999}}, }