Studying the innate immune response to myocardial infarction in a highly efficient experimental animal model
Mares, Razvan Gheorghita ; Manu, Doina ; Szabo, Istvan Adorjan ; Tomut, Mihaela Elena ; Pintican, Gabriela ; Cordos, Bogdan ; Jakobsson, Gabriel LU ; Dobreanu, Minodora ; Cotoi, Ovidiu Simion LU and Schiopu, Alexandru LU
(2021)
In Revista Romana de Cardiologie
31(3).
p.573-585
- Abstract
The reduction in mortality following acute myocardial infarction (AMI) is an important achievement of modern medicine. Despite this progress, AMI remains the most common cause of heart failure (HF) and HF-related morbidity and mortality. The involvement of the innate immune response in different stages after AMI has attracted important attention in recent years. With the increasing range of potential therapeutic compounds and delivery vectors, the need of highly efficient experimental AMI models is increasing, to support further advancement in this field. Here, we present a high-throughput model for the assessment of the innate immune response to AMI. The model is based on permanent surgical ligation of the left descending coronary... (More)
The reduction in mortality following acute myocardial infarction (AMI) is an important achievement of modern medicine. Despite this progress, AMI remains the most common cause of heart failure (HF) and HF-related morbidity and mortality. The involvement of the innate immune response in different stages after AMI has attracted important attention in recent years. With the increasing range of potential therapeutic compounds and delivery vectors, the need of highly efficient experimental AMI models is increasing, to support further advancement in this field. Here, we present a high-throughput model for the assessment of the innate immune response to AMI. The model is based on permanent surgical ligation of the left descending coronary artery (LAD) in mice, followed by complex flow-cytometry and histological analyses of immune cellular populations in blood and myocardium. We are presenting time-dependent qualitative and quantitative analysis results, demonstrating intense accumulation of Ly6Ghi neutrophils and Ly6Chi monocytes in the infarcted myocardium on days 1 and 3 post-AMI, followed by successive accumulation of reparatory Ly6CloMerTKhi macrophages, neovascularization and fibrosis development by day 7.
(Less)
- author
- Mares, Razvan Gheorghita
; Manu, Doina
; Szabo, Istvan Adorjan
; Tomut, Mihaela Elena
; Pintican, Gabriela
; Cordos, Bogdan
; Jakobsson, Gabriel
LU
; Dobreanu, Minodora
; Cotoi, Ovidiu Simion
LU
and Schiopu, Alexandru
LU
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Acute myocardial infarction, Experimental model, Innate immunity, Macrophages, Monocytes, Myocardial fibrosis, Neutrophils, Revascularization
- in
- Revista Romana de Cardiologie
- volume
- 31
- issue
- 3
- pages
- 573 - 585
- publisher
- MediaMed Publicis
- external identifiers
-
- scopus:85116054526
- ISSN
- 1220-658X
- DOI
- 10.47803/rjc.2021.31.3.573
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2021, MediaMed Publicis. All rights reserved.
- id
- a0cad84c-c95c-41ce-afc9-25e0b3af3360
- date added to LUP
- 2021-10-22 15:31:56
- date last changed
- 2024-11-17 11:39:53
@article{a0cad84c-c95c-41ce-afc9-25e0b3af3360,
abstract = {{<p>The reduction in mortality following acute myocardial infarction (AMI) is an important achievement of modern medicine. Despite this progress, AMI remains the most common cause of heart failure (HF) and HF-related morbidity and mortality. The involvement of the innate immune response in different stages after AMI has attracted important attention in recent years. With the increasing range of potential therapeutic compounds and delivery vectors, the need of highly efficient experimental AMI models is increasing, to support further advancement in this field. Here, we present a high-throughput model for the assessment of the innate immune response to AMI. The model is based on permanent surgical ligation of the left descending coronary artery (LAD) in mice, followed by complex flow-cytometry and histological analyses of immune cellular populations in blood and myocardium. We are presenting time-dependent qualitative and quantitative analysis results, demonstrating intense accumulation of Ly6G<sup>hi</sup> neutrophils and Ly6C<sup>hi</sup> monocytes in the infarcted myocardium on days 1 and 3 post-AMI, followed by successive accumulation of reparatory Ly6C<sup>lo</sup>MerTK<sup>hi</sup> macrophages, neovascularization and fibrosis development by day 7.</p>}},
author = {{Mares, Razvan Gheorghita and Manu, Doina and Szabo, Istvan Adorjan and Tomut, Mihaela Elena and Pintican, Gabriela and Cordos, Bogdan and Jakobsson, Gabriel and Dobreanu, Minodora and Cotoi, Ovidiu Simion and Schiopu, Alexandru}},
issn = {{1220-658X}},
keywords = {{Acute myocardial infarction; Experimental model; Innate immunity; Macrophages; Monocytes; Myocardial fibrosis; Neutrophils; Revascularization}},
language = {{eng}},
number = {{3}},
pages = {{573--585}},
publisher = {{MediaMed Publicis}},
series = {{Revista Romana de Cardiologie}},
title = {{Studying the innate immune response to myocardial infarction in a highly efficient experimental animal model}},
url = {{http://dx.doi.org/10.47803/rjc.2021.31.3.573}},
doi = {{10.47803/rjc.2021.31.3.573}},
volume = {{31}},
year = {{2021}},
}