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Significant impact of 5 alpha-reductase type 2 polymorphisms on sperm concentration and motility

Elzanaty, Saad LU ; Giwercman, Yvonne LU and Giwercman, Aleksander LU (2006) In International Journal of Andrology 29(3). p.414-420
Abstract
Androgens, including 5 alpha-dihydrotestosterone (DHT), are known to play a role for spermatogenesis and accessory sex gland function. The enzyme 5 alpha-reductase (SRD5A) catalyses the conversion of testosterone to DHT. Our objective was to investigate whether polymorphisms in the SRD5A2 gene influence semen parameters in the general population. DNA from 182 Swedish military conscripts was examined for the A49T, V89L, and R227Q polymorphisms in the SRD5A type 2 gene. Ejaculates were analysed according to WHO guidelines. In addition, sperm motility was assessed using computer-aided sperm analysis (CASA). Seminal markers of epididymal (neutral alpha-glucosidase), prostatic (prostate specific-antigen and zinc), and seminal vesicles function... (More)
Androgens, including 5 alpha-dihydrotestosterone (DHT), are known to play a role for spermatogenesis and accessory sex gland function. The enzyme 5 alpha-reductase (SRD5A) catalyses the conversion of testosterone to DHT. Our objective was to investigate whether polymorphisms in the SRD5A2 gene influence semen parameters in the general population. DNA from 182 Swedish military conscripts was examined for the A49T, V89L, and R227Q polymorphisms in the SRD5A type 2 gene. Ejaculates were analysed according to WHO guidelines. In addition, sperm motility was assessed using computer-aided sperm analysis (CASA). Seminal markers of epididymal (neutral alpha-glucosidase), prostatic (prostate specific-antigen and zinc), and seminal vesicles function (fructose) were measured. The A49TT-allele was associated with significantly higher sperm concentration compared with the wild type A-allele (mean: 102 x 10(6)/mL vs. 57 x 10(6)/mL, p = 0.02). The V89LV-genotype was correlated with significantly higher proportion progressive motile spermatozoa compared with the L-variant (mean: 55% vs. 48%, p = 0.04). The same trend was found regarding the CASA motile spermatozoa (mean: 52% vs. 41%, p = 0.02). No association between any of the polymorphisms and biochemical markers was found. SRD5A2 gene variants were associated with sperm concentration and motility, but not with epididymal and accessory sex gland markers. This effect on sperm parameters might therefore be exerted via a direct effect of DHT on spermatogenesis. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
polymorphism, semen parameter, SRD5A2 gene
in
International Journal of Andrology
volume
29
issue
3
pages
414 - 420
publisher
Wiley-Blackwell
external identifiers
  • wos:000237515500005
  • pmid:16487406
  • scopus:33646682640
ISSN
0105-6263
DOI
10.1111/j.1365-2605.2005.00625.x
language
English
LU publication?
yes
id
a1133f1b-7362-4188-ac6d-0964c262c137 (old id 409630)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16487406&dopt=Abstract
date added to LUP
2016-04-01 15:46:19
date last changed
2019-11-13 03:22:49
@article{a1133f1b-7362-4188-ac6d-0964c262c137,
  abstract     = {Androgens, including 5 alpha-dihydrotestosterone (DHT), are known to play a role for spermatogenesis and accessory sex gland function. The enzyme 5 alpha-reductase (SRD5A) catalyses the conversion of testosterone to DHT. Our objective was to investigate whether polymorphisms in the SRD5A2 gene influence semen parameters in the general population. DNA from 182 Swedish military conscripts was examined for the A49T, V89L, and R227Q polymorphisms in the SRD5A type 2 gene. Ejaculates were analysed according to WHO guidelines. In addition, sperm motility was assessed using computer-aided sperm analysis (CASA). Seminal markers of epididymal (neutral alpha-glucosidase), prostatic (prostate specific-antigen and zinc), and seminal vesicles function (fructose) were measured. The A49TT-allele was associated with significantly higher sperm concentration compared with the wild type A-allele (mean: 102 x 10(6)/mL vs. 57 x 10(6)/mL, p = 0.02). The V89LV-genotype was correlated with significantly higher proportion progressive motile spermatozoa compared with the L-variant (mean: 55% vs. 48%, p = 0.04). The same trend was found regarding the CASA motile spermatozoa (mean: 52% vs. 41%, p = 0.02). No association between any of the polymorphisms and biochemical markers was found. SRD5A2 gene variants were associated with sperm concentration and motility, but not with epididymal and accessory sex gland markers. This effect on sperm parameters might therefore be exerted via a direct effect of DHT on spermatogenesis.},
  author       = {Elzanaty, Saad and Giwercman, Yvonne and Giwercman, Aleksander},
  issn         = {0105-6263},
  language     = {eng},
  number       = {3},
  pages        = {414--420},
  publisher    = {Wiley-Blackwell},
  series       = {International Journal of Andrology},
  title        = {Significant impact of 5 alpha-reductase type 2 polymorphisms on sperm concentration and motility},
  url          = {http://dx.doi.org/10.1111/j.1365-2605.2005.00625.x},
  doi          = {10.1111/j.1365-2605.2005.00625.x},
  volume       = {29},
  year         = {2006},
}