Separation of rat leukocytes by countercurrent distribution in aqueous two-phase systems. II. Subpopulations which mediate selective and nonselective lysis of normal and colon carcinoma target cells in vitro
(1978) In Cellular Immunology 37(2). p.422-431- Abstract
- Spleen cells from WF rats immunized to allogeneic lymphoid cells or to syngeneic colon carcinomas and from unimmunized controls were separated by countercurrent distribution in aqueous two-phase systems. The cells were assayed for cytotoxicity to allogeneic fibroblasts or syngeneic colon carcinoma cells and to syngeneic fibroblasts in a 24-hr 51Cr-release assay. Cells from immunized rats which selectively lysed the specific target cells were repeatedly found in one area of the distribution separate from the majority of cells, which nonselectively lysed syngeneic fibroblasts as well. A similar subpopulation which nonselectively lysed all target cells assayed was recovered from the spleens of unimmunized rats. The cells were also assayed for... (More)
- Spleen cells from WF rats immunized to allogeneic lymphoid cells or to syngeneic colon carcinomas and from unimmunized controls were separated by countercurrent distribution in aqueous two-phase systems. The cells were assayed for cytotoxicity to allogeneic fibroblasts or syngeneic colon carcinoma cells and to syngeneic fibroblasts in a 24-hr 51Cr-release assay. Cells from immunized rats which selectively lysed the specific target cells were repeatedly found in one area of the distribution separate from the majority of cells, which nonselectively lysed syngeneic fibroblasts as well. A similar subpopulation which nonselectively lysed all target cells assayed was recovered from the spleens of unimmunized rats. The cells were also assayed for the ability to lyse antibody-coated thymocytes in a 4-hr 51Cr-release assay. The peak of K cells was found to overlap partially that of cells with nonselective cytotoxicity. (Less)
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https://lup.lub.lu.se/record/a1369fc9-3298-459a-a41b-57223c40b54c
- author
- Nelson, K ; Malmström, P LU ; Jönsson, Å and Sjögren, H O
- organization
- publishing date
- 1978
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Carcinoma/immunology, Cell Line, Cell Separation, Colonic Neoplasms/immunology, Countercurrent Distribution, Cytotoxicity, Immunologic, Female, Leukocytes/cytology, Neoplasms, Experimental/immunology, Rats, Rats, Inbred BN, Rats, Inbred WF, Spleen/immunology
- in
- Cellular Immunology
- volume
- 37
- issue
- 2
- pages
- 422 - 431
- publisher
- Elsevier
- external identifiers
-
- scopus:0018192431
- pmid:657286
- ISSN
- 0008-8749
- DOI
- 10.1016/0008-8749(78)90210-1
- language
- English
- LU publication?
- yes
- id
- a1369fc9-3298-459a-a41b-57223c40b54c
- date added to LUP
- 2022-02-21 12:48:22
- date last changed
- 2024-01-03 08:50:24
@article{a1369fc9-3298-459a-a41b-57223c40b54c,
abstract = {{Spleen cells from WF rats immunized to allogeneic lymphoid cells or to syngeneic colon carcinomas and from unimmunized controls were separated by countercurrent distribution in aqueous two-phase systems. The cells were assayed for cytotoxicity to allogeneic fibroblasts or syngeneic colon carcinoma cells and to syngeneic fibroblasts in a 24-hr 51Cr-release assay. Cells from immunized rats which selectively lysed the specific target cells were repeatedly found in one area of the distribution separate from the majority of cells, which nonselectively lysed syngeneic fibroblasts as well. A similar subpopulation which nonselectively lysed all target cells assayed was recovered from the spleens of unimmunized rats. The cells were also assayed for the ability to lyse antibody-coated thymocytes in a 4-hr 51Cr-release assay. The peak of K cells was found to overlap partially that of cells with nonselective cytotoxicity.}},
author = {{Nelson, K and Malmström, P and Jönsson, Å and Sjögren, H O}},
issn = {{0008-8749}},
keywords = {{Animals; Carcinoma/immunology; Cell Line; Cell Separation; Colonic Neoplasms/immunology; Countercurrent Distribution; Cytotoxicity, Immunologic; Female; Leukocytes/cytology; Neoplasms, Experimental/immunology; Rats; Rats, Inbred BN; Rats, Inbred WF; Spleen/immunology}},
language = {{eng}},
number = {{2}},
pages = {{422--431}},
publisher = {{Elsevier}},
series = {{Cellular Immunology}},
title = {{Separation of rat leukocytes by countercurrent distribution in aqueous two-phase systems. II. Subpopulations which mediate selective and nonselective lysis of normal and colon carcinoma target cells in vitro}},
url = {{http://dx.doi.org/10.1016/0008-8749(78)90210-1}},
doi = {{10.1016/0008-8749(78)90210-1}},
volume = {{37}},
year = {{1978}},
}