NMR spectroscopic properties (1H at 500 MHz) of deuterated∗ ribonucleotide-dimers ApU∗, GpC∗, partially deuterated 2′-deoxyribonucleotide-dimers d(TpA∗, d(ApT∗, d(GpC∗ and their comparison with natural counterparts (1H-NMR window)
(1993) In Journal of Biochemical and Biophysical Methods 26(1). p.1-26- Abstract
- Pure 1′#,2′,3′,4′#,5′,5′'-2H6-ribonucleoside derivatives 10–14, 1′#,2′,″,3′,4′#,5′,5″-2H7-2′-deoxynucleoside blocks 15–18 and their natural-abundance counterparts were used to asemble partially deuterated ribonucleotide-dimers (∗ indicates deuteration at 1′#,2′,3′,4′#,5′,5″(2H6)): ApU∗21, GpC∗22 partially deuterated 2′-deoxyribonucleotide-dimers d(TpA∗) 23, d(ApT∗) 25, d(GpC∗26 (∗ indicates deuteration at 1′#, 2′,2″,3′,4′#,5′,5″(2H7)) according to the procedure described by Földesi et al. (Tetradedron, in press). These five partially deuterated oligonucleotides were subsequently compred with their corresponding natural-abundance counterparts by 500 MHz 1H-NMR spectroscopy to evaluate the actual NMR simplifications achieved in the... (More)
- Pure 1′#,2′,3′,4′#,5′,5′'-2H6-ribonucleoside derivatives 10–14, 1′#,2′,″,3′,4′#,5′,5″-2H7-2′-deoxynucleoside blocks 15–18 and their natural-abundance counterparts were used to asemble partially deuterated ribonucleotide-dimers (∗ indicates deuteration at 1′#,2′,3′,4′#,5′,5″(2H6)): ApU∗21, GpC∗22 partially deuterated 2′-deoxyribonucleotide-dimers d(TpA∗) 23, d(ApT∗) 25, d(GpC∗26 (∗ indicates deuteration at 1′#, 2′,2″,3′,4′#,5′,5″(2H7)) according to the procedure described by Földesi et al. (Tetradedron, in press). These five partially deuterated oligonucleotides were subsequently compred with their corresponding natural-abundance counterparts by 500 MHz 1H-NMR spectroscopy to evaluate the actual NMR simplifications achieved in the non-deuterated part (1H-NMR window) as a result of specific deuterium incorporation. Detailed one-dimensional 1H-NMR (500 MHz), two-dimensional correlation spectra (DQF-COSY and TOCSY) and deuterium isotope effect on the chemical shifts of oligonucleotides have been presented. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/a138e740-db89-4d08-9f52-19812eb4935d
- author
- Földesi, A. ; Nilson, F.P.R. LU ; Glemarec, C. ; Gioeli, C. and Chattopadhyaya, J.
- publishing date
- 1993-02
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- H-NMR spectroscopy, Deuterated ribonucleotide dimer, Deuterated 2′-deoxyribonucleotide dimer
- in
- Journal of Biochemical and Biophysical Methods
- volume
- 26
- issue
- 1
- pages
- 26 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0027462913
- ISSN
- 0165-022X
- DOI
- 10.1016/0165-022X(93)90018-J
- language
- English
- LU publication?
- no
- id
- a138e740-db89-4d08-9f52-19812eb4935d
- date added to LUP
- 2019-09-28 17:07:20
- date last changed
- 2021-01-03 09:16:54
@article{a138e740-db89-4d08-9f52-19812eb4935d,
abstract = {{Pure 1′#,2′,3′,4′#,5′,5′'-2H6-ribonucleoside derivatives 10–14, 1′#,2′,″,3′,4′#,5′,5″-2H7-2′-deoxynucleoside blocks 15–18 and their natural-abundance counterparts were used to asemble partially deuterated ribonucleotide-dimers (∗ indicates deuteration at 1′#,2′,3′,4′#,5′,5″(2H6)): ApU∗21, GpC∗22 partially deuterated 2′-deoxyribonucleotide-dimers d(TpA∗) 23, d(ApT∗) 25, d(GpC∗26 (∗ indicates deuteration at 1′#, 2′,2″,3′,4′#,5′,5″(2H7)) according to the procedure described by Földesi et al. (Tetradedron, in press). These five partially deuterated oligonucleotides were subsequently compred with their corresponding natural-abundance counterparts by 500 MHz 1H-NMR spectroscopy to evaluate the actual NMR simplifications achieved in the non-deuterated part (1H-NMR window) as a result of specific deuterium incorporation. Detailed one-dimensional 1H-NMR (500 MHz), two-dimensional correlation spectra (DQF-COSY and TOCSY) and deuterium isotope effect on the chemical shifts of oligonucleotides have been presented.}},
author = {{Földesi, A. and Nilson, F.P.R. and Glemarec, C. and Gioeli, C. and Chattopadhyaya, J.}},
issn = {{0165-022X}},
keywords = {{H-NMR spectroscopy, Deuterated ribonucleotide dimer, Deuterated 2′-deoxyribonucleotide dimer}},
language = {{eng}},
number = {{1}},
pages = {{1--26}},
publisher = {{Elsevier}},
series = {{Journal of Biochemical and Biophysical Methods}},
title = {{NMR spectroscopic properties (1H at 500 MHz) of deuterated∗ ribonucleotide-dimers ApU∗, GpC∗, partially deuterated 2′-deoxyribonucleotide-dimers d(TpA∗, d(ApT∗, d(GpC∗ and their comparison with natural counterparts (1H-NMR window)}},
url = {{http://dx.doi.org/10.1016/0165-022X(93)90018-J}},
doi = {{10.1016/0165-022X(93)90018-J}},
volume = {{26}},
year = {{1993}},
}