Fast-tracking of single emitters in large volumes with nanometer precision
(2020) In Optics Express 28(19). p.28656-28671- Abstract
Multifocal plane microscopy allows for capturing images at different focal planes simultaneously. Using a proprietary prism which splits the emitted light into paths of different lengths, images at 8 different focal depths were obtained, covering a volume of 50x50x4 μm3. The position of single emitters was retrieved using a phasor-based approach across the different imaging planes, with better than 10 nm precision in the axial direction. We validated the accuracy of this approach by tracking fluorescent beads in 3D to calculate water viscosity. The fast acquisition rate (>100 fps) also enabled us to follow the capturing of 0.2 μm fluorescent beads into an optical trap.
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https://lup.lub.lu.se/record/a1c27d26-2d61-4d03-a9ec-a74f12d9e756
- author
- LOUIS, BORIS LU ; CAMACHO, RAFAEL ; BRESOLÍ-OBACH, ROGER ; ABAKUMOV, SERGEY ; VANDAELE, JOHANNES ; KUDO, TESTSUHIRO ; MASUHARA, HIROSHI ; SCHEBLYKIN, IVAN G. LU ; HOFKENS, JOHAN and ROCHA, SUSANA
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Optics Express
- volume
- 28
- issue
- 19
- article number
- 401557
- pages
- 16 pages
- publisher
- Optical Society of America
- external identifiers
-
- pmid:32988132
- scopus:85091845720
- ISSN
- 1094-4087
- DOI
- 10.1364/OE.401557
- language
- English
- LU publication?
- yes
- id
- a1c27d26-2d61-4d03-a9ec-a74f12d9e756
- date added to LUP
- 2020-11-02 13:22:59
- date last changed
- 2024-08-22 05:39:01
@article{a1c27d26-2d61-4d03-a9ec-a74f12d9e756, abstract = {{<p>Multifocal plane microscopy allows for capturing images at different focal planes simultaneously. Using a proprietary prism which splits the emitted light into paths of different lengths, images at 8 different focal depths were obtained, covering a volume of 50x50x4 μm3. The position of single emitters was retrieved using a phasor-based approach across the different imaging planes, with better than 10 nm precision in the axial direction. We validated the accuracy of this approach by tracking fluorescent beads in 3D to calculate water viscosity. The fast acquisition rate (>100 fps) also enabled us to follow the capturing of 0.2 μm fluorescent beads into an optical trap.</p>}}, author = {{LOUIS, BORIS and CAMACHO, RAFAEL and BRESOLÍ-OBACH, ROGER and ABAKUMOV, SERGEY and VANDAELE, JOHANNES and KUDO, TESTSUHIRO and MASUHARA, HIROSHI and SCHEBLYKIN, IVAN G. and HOFKENS, JOHAN and ROCHA, SUSANA}}, issn = {{1094-4087}}, language = {{eng}}, number = {{19}}, pages = {{28656--28671}}, publisher = {{Optical Society of America}}, series = {{Optics Express}}, title = {{Fast-tracking of single emitters in large volumes with nanometer precision}}, url = {{http://dx.doi.org/10.1364/OE.401557}}, doi = {{10.1364/OE.401557}}, volume = {{28}}, year = {{2020}}, }