Exploring d-xylose oxidation in Saccharomyces cerevisiae through the Weimberg pathway
(2018) In AMB Express 8(1).- Abstract
- Engineering of the yeast Saccharomyces cerevisiae towards efficient d-xylose assimilation has been a major focus over the last decades since d-xylose is the second most abundant sugar in nature, and its conversion into products could significantly improve process economy in biomass-based processes. Up to now, two different metabolic routes have been introduced via genetic engineering, consisting of either the isomerization or the oxido-reduction of d-xylose to d-xylulose that is further connected to the pentose phosphate pathway and glycolysis. In the present study, cytosolic d-xylose oxidation was investigated instead, through the introduction of the Weimberg pathway from Caulobacter crescentus in S. cerevisiae. This pathway consists of... (More)
- Engineering of the yeast Saccharomyces cerevisiae towards efficient d-xylose assimilation has been a major focus over the last decades since d-xylose is the second most abundant sugar in nature, and its conversion into products could significantly improve process economy in biomass-based processes. Up to now, two different metabolic routes have been introduced via genetic engineering, consisting of either the isomerization or the oxido-reduction of d-xylose to d-xylulose that is further connected to the pentose phosphate pathway and glycolysis. In the present study, cytosolic d-xylose oxidation was investigated instead, through the introduction of the Weimberg pathway from Caulobacter crescentus in S. cerevisiae. This pathway consists of five reaction steps that connect d-xylose to the TCA cycle intermediate α-ketoglutarate. The corresponding genes could be expressed in S. cerevisiae, but no growth was observed on d-xylose indicating that not all the enzymes were functionally active. The accumulation of the Weimberg intermediate d-xylonate suggested that the dehydration step(s) might be limiting, blocking further conversion into α-ketoglutarate. Although four alternative dehydratases both of bacterial and archaeon origins were evaluated, d-xylonate accumulation still occurred. A better understanding of the mechanisms associated with the activity of dehydratases, both at a bacterial and yeast level, appears essential to obtain a fully functional Weimberg pathway in S. cerevisiae. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/a1d236db-4c47-49b3-bcf7-fb4adb4c26ab
- author
- Wasserstrom, Lisa LU ; Portugal-Nunes, Diogo LU ; Almqvist, Henrik LU ; Sandström, Anders LU ; Lidén, Gunnar LU and Gorwa-Grauslund, Marie F LU
- organization
- publishing date
- 2018-03-05
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- D-Xylose, Weimberg pathway, Saccharomyces cerevisiae, D-Xylonate dehydratase, Caulobacter crescentus, Iron–sulfur clusters
- in
- AMB Express
- volume
- 8
- issue
- 1
- article number
- 8:33
- publisher
- Springer
- external identifiers
-
- scopus:85043234829
- pmid:29508097
- ISSN
- 2191-0855
- DOI
- 10.1186/s13568-018-0564-9
- language
- English
- LU publication?
- yes
- id
- a1d236db-4c47-49b3-bcf7-fb4adb4c26ab
- date added to LUP
- 2018-03-06 09:54:32
- date last changed
- 2023-12-02 04:12:27
@article{a1d236db-4c47-49b3-bcf7-fb4adb4c26ab,
abstract = {{Engineering of the yeast Saccharomyces cerevisiae towards efficient d-xylose assimilation has been a major focus over the last decades since d-xylose is the second most abundant sugar in nature, and its conversion into products could significantly improve process economy in biomass-based processes. Up to now, two different metabolic routes have been introduced via genetic engineering, consisting of either the isomerization or the oxido-reduction of d-xylose to d-xylulose that is further connected to the pentose phosphate pathway and glycolysis. In the present study, cytosolic d-xylose oxidation was investigated instead, through the introduction of the Weimberg pathway from Caulobacter crescentus in S. cerevisiae. This pathway consists of five reaction steps that connect d-xylose to the TCA cycle intermediate α-ketoglutarate. The corresponding genes could be expressed in S. cerevisiae, but no growth was observed on d-xylose indicating that not all the enzymes were functionally active. The accumulation of the Weimberg intermediate d-xylonate suggested that the dehydration step(s) might be limiting, blocking further conversion into α-ketoglutarate. Although four alternative dehydratases both of bacterial and archaeon origins were evaluated, d-xylonate accumulation still occurred. A better understanding of the mechanisms associated with the activity of dehydratases, both at a bacterial and yeast level, appears essential to obtain a fully functional Weimberg pathway in S. cerevisiae.}},
author = {{Wasserstrom, Lisa and Portugal-Nunes, Diogo and Almqvist, Henrik and Sandström, Anders and Lidén, Gunnar and Gorwa-Grauslund, Marie F}},
issn = {{2191-0855}},
keywords = {{D-Xylose; Weimberg pathway; Saccharomyces cerevisiae; D-Xylonate dehydratase; Caulobacter crescentus; Iron–sulfur clusters}},
language = {{eng}},
month = {{03}},
number = {{1}},
publisher = {{Springer}},
series = {{AMB Express}},
title = {{Exploring d-xylose oxidation in Saccharomyces cerevisiae through the Weimberg pathway}},
url = {{http://dx.doi.org/10.1186/s13568-018-0564-9}},
doi = {{10.1186/s13568-018-0564-9}},
volume = {{8}},
year = {{2018}},
}