Advanced

Continuous lipid bilayers derived from cell membranes for spatial molecular manipulation

Simonsson, Lisa LU ; Gunnarsson, Anders LU ; Wallin, Patric ; Jönsson, Peter LU and Höök, Fredrik LU (2011) In Journal of the American Chemical Society 133(35). p.32-14027
Abstract

Progress with respect to enrichment and separation of native membrane components in complex lipid environments, such as native cell membranes, has so far been very limited. The reason for the slow progress can be related to the lack of efficient means to generate continuous and laterally fluid supported lipid bilayers (SLBs) made from real cell membranes. We show in this work how the edge of a hydrodynamically driven SLB can be used to induce rupture of adsorbed lipid vesicles of compositions that typically prevent spontaneous SLB formation, such as vesicles made of complex lipid compositions, containing high cholesterol content or being derived from real cell membranes. In particular, upon fusion between the moving edge of a preformed... (More)

Progress with respect to enrichment and separation of native membrane components in complex lipid environments, such as native cell membranes, has so far been very limited. The reason for the slow progress can be related to the lack of efficient means to generate continuous and laterally fluid supported lipid bilayers (SLBs) made from real cell membranes. We show in this work how the edge of a hydrodynamically driven SLB can be used to induce rupture of adsorbed lipid vesicles of compositions that typically prevent spontaneous SLB formation, such as vesicles made of complex lipid compositions, containing high cholesterol content or being derived from real cell membranes. In particular, upon fusion between the moving edge of a preformed SLB and adsorbed vesicles made directly from 3T3 fibroblast cell membranes, the membrane content of the vesicles was shown to be efficiently transferred to the SLB. The molecular transfer was verified using cholera toxin B subunit (CTB) binding to monosialoganglioside receptors (G(M1) and G(M3)), and the preserved lateral mobility was confirmed by spatial manipulation of the G(M1/M3)-CTB complex using a hydrodynamic flow. Two populations of CTB with markedly different drift velocity could be identified, which from dissociation kinetics data were attributed to CTB bound with different numbers of ganglioside anchors.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
3T3 Cells, Animals, Cell Membrane, Cholera Toxin, Cholesterol, Fibroblasts, Hydrodynamics, Lipid Bilayers, Membrane Fusion, Mice, Micromanipulation, Protein Binding, Receptors, Cell Surface, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of the American Chemical Society
volume
133
issue
35
pages
6 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:80052341509
  • pmid:21786792
ISSN
1520-5126
DOI
10.1021/ja204589a
language
English
LU publication?
no
id
a1dd1276-7687-4e50-bff2-e4cf92d821fa
date added to LUP
2018-01-26 10:43:42
date last changed
2020-08-26 04:07:32
@article{a1dd1276-7687-4e50-bff2-e4cf92d821fa,
  abstract     = {<p>Progress with respect to enrichment and separation of native membrane components in complex lipid environments, such as native cell membranes, has so far been very limited. The reason for the slow progress can be related to the lack of efficient means to generate continuous and laterally fluid supported lipid bilayers (SLBs) made from real cell membranes. We show in this work how the edge of a hydrodynamically driven SLB can be used to induce rupture of adsorbed lipid vesicles of compositions that typically prevent spontaneous SLB formation, such as vesicles made of complex lipid compositions, containing high cholesterol content or being derived from real cell membranes. In particular, upon fusion between the moving edge of a preformed SLB and adsorbed vesicles made directly from 3T3 fibroblast cell membranes, the membrane content of the vesicles was shown to be efficiently transferred to the SLB. The molecular transfer was verified using cholera toxin B subunit (CTB) binding to monosialoganglioside receptors (G(M1) and G(M3)), and the preserved lateral mobility was confirmed by spatial manipulation of the G(M1/M3)-CTB complex using a hydrodynamic flow. Two populations of CTB with markedly different drift velocity could be identified, which from dissociation kinetics data were attributed to CTB bound with different numbers of ganglioside anchors.</p>},
  author       = {Simonsson, Lisa and Gunnarsson, Anders and Wallin, Patric and Jönsson, Peter and Höök, Fredrik},
  issn         = {1520-5126},
  language     = {eng},
  month        = {09},
  number       = {35},
  pages        = {32--14027},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Journal of the American Chemical Society},
  title        = {Continuous lipid bilayers derived from cell membranes for spatial molecular manipulation},
  url          = {http://dx.doi.org/10.1021/ja204589a},
  doi          = {10.1021/ja204589a},
  volume       = {133},
  year         = {2011},
}