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A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1

Böiers, Charlotta LU ; Richardson, Simon E. ; Laycock, Emma ; Zriwil, Alya LU ; Turati, Virginia A. ; Brown, John ; Wray, Jason P. ; Wang, Dapeng ; James, Chela and Herrero, Javier , et al. (2018) In Developmental Cell 44(3). p.7-377
Abstract

ETV6-RUNX1 is associated with childhood acute B-lymphoblastic leukemia (cALL) functioning as a first-hit mutation that initiates a clinically silent pre-leukemia in utero. Because lineage commitment hierarchies differ between embryo and adult, and the impact of oncogenes is cell-context dependent, we hypothesized that the childhood affiliation of ETV6-RUNX1 cALL reflects its origins in a progenitor unique to embryonic life. We characterize the first emerging B cells in first-trimester human embryos, identifying a developmentally restricted CD19−IL-7R+ progenitor compartment, which transitions from a myeloid to lymphoid program during ontogeny. This developmental series is recapitulated in differentiating human... (More)

ETV6-RUNX1 is associated with childhood acute B-lymphoblastic leukemia (cALL) functioning as a first-hit mutation that initiates a clinically silent pre-leukemia in utero. Because lineage commitment hierarchies differ between embryo and adult, and the impact of oncogenes is cell-context dependent, we hypothesized that the childhood affiliation of ETV6-RUNX1 cALL reflects its origins in a progenitor unique to embryonic life. We characterize the first emerging B cells in first-trimester human embryos, identifying a developmentally restricted CD19−IL-7R+ progenitor compartment, which transitions from a myeloid to lymphoid program during ontogeny. This developmental series is recapitulated in differentiating human pluripotent stem cells (hPSCs), thereby providing a model for the initiation of cALL. Genome-engineered hPSCs expressing ETV6-RUNX1 from the endogenous ETV6 locus show expansion of the CD19−IL-7R+ compartment, show a partial block in B lineage commitment, and produce proB cells with aberrant myeloid gene expression signatures and potential: features (collectively) consistent with a pre-leukemic state. Böiers, Richardson et al. explore the potential for a developmental susceptibility to childhood acute lymphoblastic leukemia. Characterization of earliest B cell progenitors in human fetal liver identified a unique progenitor compartment that can be recapitulated using human pluripotent stem cells to model the impact of the pre-leukemia-initiating oncogene ETV6-RUNX1.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
acute lymphoblastic leukemia, B cell, CRISPR/Cas9, ETV6-RUNX1, genome engineering, human fetal lymphopoiesis, human pluripotent stem cells, in vitro B cell differentiation
in
Developmental Cell
volume
44
issue
3
pages
7 - 377
publisher
Cell Press
external identifiers
  • scopus:85044733881
  • pmid:29290585
ISSN
1534-5807
DOI
10.1016/j.devcel.2017.12.005
language
English
LU publication?
yes
id
a1eb465d-76ee-489e-8220-7f4476b62dcd
date added to LUP
2018-04-11 14:22:40
date last changed
2024-05-13 08:22:44
@article{a1eb465d-76ee-489e-8220-7f4476b62dcd,
  abstract     = {{<p>ETV6-RUNX1 is associated with childhood acute B-lymphoblastic leukemia (cALL) functioning as a first-hit mutation that initiates a clinically silent pre-leukemia in utero. Because lineage commitment hierarchies differ between embryo and adult, and the impact of oncogenes is cell-context dependent, we hypothesized that the childhood affiliation of ETV6-RUNX1 cALL reflects its origins in a progenitor unique to embryonic life. We characterize the first emerging B cells in first-trimester human embryos, identifying a developmentally restricted CD19<sup>−</sup>IL-7R<sup>+</sup> progenitor compartment, which transitions from a myeloid to lymphoid program during ontogeny. This developmental series is recapitulated in differentiating human pluripotent stem cells (hPSCs), thereby providing a model for the initiation of cALL. Genome-engineered hPSCs expressing ETV6-RUNX1 from the endogenous ETV6 locus show expansion of the CD19<sup>−</sup>IL-7R<sup>+</sup> compartment, show a partial block in B lineage commitment, and produce proB cells with aberrant myeloid gene expression signatures and potential: features (collectively) consistent with a pre-leukemic state. Böiers, Richardson et al. explore the potential for a developmental susceptibility to childhood acute lymphoblastic leukemia. Characterization of earliest B cell progenitors in human fetal liver identified a unique progenitor compartment that can be recapitulated using human pluripotent stem cells to model the impact of the pre-leukemia-initiating oncogene ETV6-RUNX1.</p>}},
  author       = {{Böiers, Charlotta and Richardson, Simon E. and Laycock, Emma and Zriwil, Alya and Turati, Virginia A. and Brown, John and Wray, Jason P. and Wang, Dapeng and James, Chela and Herrero, Javier and Sitnicka, Ewa and Karlsson, Stefan and Smith, Andrew J.H. and Jacobsen, Sten Erik W. and Enver, Tariq}},
  issn         = {{1534-5807}},
  keywords     = {{acute lymphoblastic leukemia; B cell; CRISPR/Cas9; ETV6-RUNX1; genome engineering; human fetal lymphopoiesis; human pluripotent stem cells; in vitro B cell differentiation}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{3}},
  pages        = {{7--377}},
  publisher    = {{Cell Press}},
  series       = {{Developmental Cell}},
  title        = {{A Human IPS Model Implicates Embryonic B-Myeloid Fate Restriction as Developmental Susceptibility to B Acute Lymphoblastic Leukemia-Associated ETV6-RUNX1}},
  url          = {{http://dx.doi.org/10.1016/j.devcel.2017.12.005}},
  doi          = {{10.1016/j.devcel.2017.12.005}},
  volume       = {{44}},
  year         = {{2018}},
}