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Effect of acute hypohydration on glycemic regulation in healthy adults : A randomized crossover trial

Carroll, Harriet A.; Templeman, Iain; Chen, Yung Chih; Edinburgh, Robert M.; Burch, Elaine K.; Jewitt, Jake T.; Povey, Georgie; Robinson, Timothy D.; Dooley, William L. and Jones, Robert, et al. (2019) In Journal of Applied Physiology 126(2). p.422-430
Abstract


he aim of this study was to investigate the acute effect of hydration status on glycemic regulation in healthy adults and explore underlying mechanisms. In this randomized crossover trial, 16 healthy adults (8 men, 8 women) underwent an oral glucose tolerance test (OGTT) when hypohydrated and rehydrated after 4 days of pretrial standardization. One day before OGTT, participants were dehydrated for 1 h in a heat tent with subsequent fluid restriction (HYPO) or replacement (RE). The following day, an OGTT was performed with metabolic rate measurements and pre- and post-OGTT muscle biopsies. Peripheral quantitative computer tomography thigh scans were taken before and after... (More)


he aim of this study was to investigate the acute effect of hydration status on glycemic regulation in healthy adults and explore underlying mechanisms. In this randomized crossover trial, 16 healthy adults (8 men, 8 women) underwent an oral glucose tolerance test (OGTT) when hypohydrated and rehydrated after 4 days of pretrial standardization. One day before OGTT, participants were dehydrated for 1 h in a heat tent with subsequent fluid restriction (HYPO) or replacement (RE). The following day, an OGTT was performed with metabolic rate measurements and pre- and post-OGTT muscle biopsies. Peripheral quantitative computer tomography thigh scans were taken before and after intervention to infer changes in cell volume. HYPO (but not RE) induced 1.9% (SD 1.2) body mass loss, 2.9% (SD 2.7) cell volume reduction, and increased urinary hydration markers, serum osmolality, and plasma copeptin concentration (all P 0.007). Fasted serum glucose [HYPO 5.10 mmol/l (SD 0.42), RE 5.02 mmol/l (SD 0.40); P 0.327] and insulin [HYPO 27.1 pmol/l (SD 9.7), RE 27.6 pmol/l (SD 9.2); P 0.809] concentrations were similar between HYPO and RE. Hydration status did not alter the serum glucose (P 0.627) or insulin (P 0.200) responses during the OGTT. Muscle water content was lower before OGTT after HYPO compared with RE [761 g/kg wet wt (SD 13) vs. 772 g/kg wet wt (SD 18) RE] but similar after OGTT [HYPO 779 g/kg wet wt (SD 15) vs. RE 780 g/kg wet wt (SD 20); time P 0.011; trial time P 0.055]. Resting energy expenditure was similar between hydration states (stable between 1.21 and 5.94 kJ·kg
1
·day
1
; trial P 0.904). Overall, despite acute mild hypohydration increasing plasma copeptin concentrations and decreasing fasted cell volume and muscle water, we found no effect on glycemic regulation.

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Journal of Applied Physiology
volume
126
issue
2
pages
9 pages
publisher
American Physiological Society
external identifiers
  • scopus:85061963276
ISSN
8750-7587
DOI
10.1152/japplphysiol.00771.2018
language
English
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yes
id
a20e8577-efef-4eec-8e60-4a1404e8e390
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2019-03-06 13:23:58
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2019-07-16 04:05:33
@article{a20e8577-efef-4eec-8e60-4a1404e8e390,
  abstract     = {<p><br>
                                                         he aim of this study was to investigate the acute effect of hydration status on glycemic regulation in healthy adults and explore underlying mechanisms. In this randomized crossover trial, 16 healthy adults (8 men, 8 women) underwent an oral glucose tolerance test (OGTT) when hypohydrated and rehydrated after 4 days of pretrial standardization. One day before OGTT, participants were dehydrated for 1 h in a heat tent with subsequent fluid restriction (HYPO) or replacement (RE). The following day, an OGTT was performed with metabolic rate measurements and pre- and post-OGTT muscle biopsies. Peripheral quantitative computer tomography thigh scans were taken before and after intervention to infer changes in cell volume. HYPO (but not RE) induced 1.9% (SD 1.2) body mass loss, 2.9% (SD 2.7) cell volume reduction, and increased urinary hydration markers, serum osmolality, and plasma copeptin concentration (all P 0.007). Fasted serum glucose [HYPO 5.10 mmol/l (SD 0.42), RE 5.02 mmol/l (SD 0.40); P 0.327] and insulin [HYPO 27.1 pmol/l (SD 9.7), RE 27.6 pmol/l (SD 9.2); P 0.809] concentrations were similar between HYPO and RE. Hydration status did not alter the serum glucose (P 0.627) or insulin (P 0.200) responses during the OGTT. Muscle water content was lower before OGTT after HYPO compared with RE [761 g/kg wet wt (SD 13) vs. 772 g/kg wet wt (SD 18) RE] but similar after OGTT [HYPO 779 g/kg wet wt (SD 15) vs. RE 780 g/kg wet wt (SD 20); time P 0.011; trial time P 0.055]. Resting energy expenditure was similar between hydration states (stable between 1.21 and 5.94 kJ·kg                             <br>
                            <sup>1</sup><br>
                                                         ·day                             <br>
                            <sup>1</sup><br>
                                                         ; trial P 0.904). Overall, despite acute mild hypohydration increasing plasma copeptin concentrations and decreasing fasted cell volume and muscle water, we found no effect on glycemic regulation.                         <br>
                        </p>},
  author       = {Carroll, Harriet A. and Templeman, Iain and Chen, Yung Chih and Edinburgh, Robert M. and Burch, Elaine K. and Jewitt, Jake T. and Povey, Georgie and Robinson, Timothy D. and Dooley, William L. and Jones, Robert and Tsintzas, Kostas and Gallo, Widet and Melander, Olle and Thompson, Dylan and James, Lewis J. and Johnson, Laura and Betts, James A.},
  issn         = {8750-7587},
  language     = {eng},
  number       = {2},
  pages        = {422--430},
  publisher    = {American Physiological Society},
  series       = {Journal of Applied Physiology},
  title        = {Effect of acute hypohydration on glycemic regulation in healthy adults : A randomized crossover trial},
  url          = {http://dx.doi.org/10.1152/japplphysiol.00771.2018},
  volume       = {126},
  year         = {2019},
}