Non-lethal sampling for assessment of mitochondrial function does not affect metabolic rate and swimming performance
(2024) In Philosophical Transactions of the Royal Society B: Biological Sciences 379(1896).- Abstract
A fundamental issue in the metabolic field is whether it is possible to understand underlying mechanisms that characterize individual variation. Whole-animal performance relies on mitochondrial function as it produces energy for cellular processes. However, our lack of longitudinal measures to evaluate how mitochondrial function can change within and among individuals and with environmental context makes it difficult to assess individual variation in mitochondrial traits. The aims of this study were to test the repeatability of muscle mitochondrial metabolism by performing two biopsies of red muscle, and to evaluate the effects of biopsies on whole-animal performance in goldfish Carassius auratus. Our results show that basal... (More)
A fundamental issue in the metabolic field is whether it is possible to understand underlying mechanisms that characterize individual variation. Whole-animal performance relies on mitochondrial function as it produces energy for cellular processes. However, our lack of longitudinal measures to evaluate how mitochondrial function can change within and among individuals and with environmental context makes it difficult to assess individual variation in mitochondrial traits. The aims of this study were to test the repeatability of muscle mitochondrial metabolism by performing two biopsies of red muscle, and to evaluate the effects of biopsies on whole-animal performance in goldfish Carassius auratus. Our results show that basal mitochondrial respiration and net phosphorylation efficiency are repeatable at 14-day intervals. We also show that swimming performance (optimal cost of transport and critical swimming speed) was repeatable in biopsied fish, whereas the repeatability of individual oxygen consumption (standard and maximal metabolic rates) seemed unstable over time. However, we noted that the means of individual and mitochondrial traits did not change over time in biopsied fish. This study shows that muscle biopsies allow the measurement of mitochondrial metabolism without sacrificing animals and that two muscle biopsies 14 days apart affect the intraspecific variation in fish performance without affecting average performance of individuals. This article is part of the theme issue 'The evolutionary significance of variation in metabolic rates'.
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- author
- Thoral, Elisa LU ; Dargère, Lauréliane ; Medina-Suárez, Ione ; Clair, Angéline ; Averty, Laetitia ; Sigaud, Justine ; Morales, Anne ; Salin, Karine and Teulier, Loïc
- organization
- publishing date
- 2024-02-26
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- goldfish, red muscle, repeatability, whole-animal performance
- in
- Philosophical Transactions of the Royal Society B: Biological Sciences
- volume
- 379
- issue
- 1896
- article number
- 20220483
- pages
- 9 pages
- publisher
- Royal Society Publishing
- external identifiers
-
- pmid:38186271
- scopus:85182029937
- ISSN
- 0962-8436
- DOI
- 10.1098/rstb.2022.0483
- language
- English
- LU publication?
- yes
- id
- a21e8bdc-86d7-4388-a7f4-bea73c729822
- date added to LUP
- 2024-02-20 15:12:11
- date last changed
- 2024-04-20 16:12:09
@article{a21e8bdc-86d7-4388-a7f4-bea73c729822,
abstract = {{<p>A fundamental issue in the metabolic field is whether it is possible to understand underlying mechanisms that characterize individual variation. Whole-animal performance relies on mitochondrial function as it produces energy for cellular processes. However, our lack of longitudinal measures to evaluate how mitochondrial function can change within and among individuals and with environmental context makes it difficult to assess individual variation in mitochondrial traits. The aims of this study were to test the repeatability of muscle mitochondrial metabolism by performing two biopsies of red muscle, and to evaluate the effects of biopsies on whole-animal performance in goldfish Carassius auratus. Our results show that basal mitochondrial respiration and net phosphorylation efficiency are repeatable at 14-day intervals. We also show that swimming performance (optimal cost of transport and critical swimming speed) was repeatable in biopsied fish, whereas the repeatability of individual oxygen consumption (standard and maximal metabolic rates) seemed unstable over time. However, we noted that the means of individual and mitochondrial traits did not change over time in biopsied fish. This study shows that muscle biopsies allow the measurement of mitochondrial metabolism without sacrificing animals and that two muscle biopsies 14 days apart affect the intraspecific variation in fish performance without affecting average performance of individuals. This article is part of the theme issue 'The evolutionary significance of variation in metabolic rates'.</p>}},
author = {{Thoral, Elisa and Dargère, Lauréliane and Medina-Suárez, Ione and Clair, Angéline and Averty, Laetitia and Sigaud, Justine and Morales, Anne and Salin, Karine and Teulier, Loïc}},
issn = {{0962-8436}},
keywords = {{goldfish; red muscle; repeatability; whole-animal performance}},
language = {{eng}},
month = {{02}},
number = {{1896}},
publisher = {{Royal Society Publishing}},
series = {{Philosophical Transactions of the Royal Society B: Biological Sciences}},
title = {{Non-lethal sampling for assessment of mitochondrial function does not affect metabolic rate and swimming performance}},
url = {{http://dx.doi.org/10.1098/rstb.2022.0483}},
doi = {{10.1098/rstb.2022.0483}},
volume = {{379}},
year = {{2024}},
}