Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Quantitative studies of the binding of the class II PapG adhesin from uropathogenic Escherichia coli to oligosaccharides.

Larsson, A ; Ohlsson, Jörgen LU ; Dodson, K W ; Hultgren, S J ; Nilsson, Ulf LU and Kihlberg, J (2003) In Bioorganic & Medicinal Chemistry 11(10). p.2255-2261
Abstract
Binding of the class II PapG adhesin, found at the tip of filamentous pili on Escherichia coli, to the carbohydrate moiety of globoseries glycolipids in the human kidney is a key step in development of pyelonephritis, a severe form of urinary tract infection. An assay based on surface plasmon resonance for quantification of the binding of the class II PapG adhesin to oligosaccharides has been developed. Using this assay dissociation constants ranging from 80 to 540 M were determined for binding of the PapG adhesin to di-pentasaccharide fragments from the globoseries of glycolipids. A series of galabiose derivatives, modified at the anomeric position, O-2′ or O-3′, was also investigated. The anomeric position appeared to be the most... (More)
Binding of the class II PapG adhesin, found at the tip of filamentous pili on Escherichia coli, to the carbohydrate moiety of globoseries glycolipids in the human kidney is a key step in development of pyelonephritis, a severe form of urinary tract infection. An assay based on surface plasmon resonance for quantification of the binding of the class II PapG adhesin to oligosaccharides has been developed. Using this assay dissociation constants ranging from 80 to 540 M were determined for binding of the PapG adhesin to di-pentasaccharide fragments from the globoseries of glycolipids. A series of galabiose derivatives, modified at the anomeric position, O-2′ or O-3′, was also investigated. The anomeric position appeared to be the most promising for development of improved inhibitors of PapG-mediated adhesion of E. coli. p-Methoxyphenyl galabioside was found to be most potent (Kd=140 M), and binds to PapG almost as well as the Forssman pentasaccharide. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioorganic & Medicinal Chemistry
volume
11
issue
10
pages
2255 - 2261
publisher
Elsevier
external identifiers
  • wos:000182794200014
  • pmid:12713835
  • scopus:0344490325
ISSN
0968-0896
DOI
10.1016/S0968-0896(03)00114-7
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Organic chemistry (S/LTH) (011001240)
id
a23df687-bbf6-47d8-aec1-7129e2253830 (old id 127945)
date added to LUP
2016-04-01 12:05:57
date last changed
2022-01-26 22:46:21
@article{a23df687-bbf6-47d8-aec1-7129e2253830,
  abstract     = {{Binding of the class II PapG adhesin, found at the tip of filamentous pili on Escherichia coli, to the carbohydrate moiety of globoseries glycolipids in the human kidney is a key step in development of pyelonephritis, a severe form of urinary tract infection. An assay based on surface plasmon resonance for quantification of the binding of the class II PapG adhesin to oligosaccharides has been developed. Using this assay dissociation constants ranging from 80 to 540 M were determined for binding of the PapG adhesin to di-pentasaccharide fragments from the globoseries of glycolipids. A series of galabiose derivatives, modified at the anomeric position, O-2′ or O-3′, was also investigated. The anomeric position appeared to be the most promising for development of improved inhibitors of PapG-mediated adhesion of E. coli. p-Methoxyphenyl galabioside was found to be most potent (Kd=140 M), and binds to PapG almost as well as the Forssman pentasaccharide.}},
  author       = {{Larsson, A and Ohlsson, Jörgen and Dodson, K W and Hultgren, S J and Nilsson, Ulf and Kihlberg, J}},
  issn         = {{0968-0896}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{2255--2261}},
  publisher    = {{Elsevier}},
  series       = {{Bioorganic & Medicinal Chemistry}},
  title        = {{Quantitative studies of the binding of the class II PapG adhesin from uropathogenic Escherichia coli to oligosaccharides.}},
  url          = {{http://dx.doi.org/10.1016/S0968-0896(03)00114-7}},
  doi          = {{10.1016/S0968-0896(03)00114-7}},
  volume       = {{11}},
  year         = {{2003}},
}