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Simultaneous immunohistochemistry and autoradiography of peptides and 5-hydroxytryptamine in bird retina

Tornqvist, K LU and Ehinger, B LU orcid (1983) In Neurochemistry International 5(5). p.86-579
Abstract

A method was developed for the simultaneous demonstration of two specific cellular constituents. Cryostat sections of formaldehyde fixed tissue from retinae treated with ((3)H)-5-hydroxytryptamine were subjected to immunohistochemistry and subsequent autoradiography. The method takes advantage of the very efficient and specific uptake mechanism that many types of neurons possess which makes it possible to label them with radioactivity. With this method a study was made on the possible co-occurrence in the avian retina of 5-hydroxytryptamine on one hand and somatostatin, glucagon and substance P on the other. Substance P and 5-hydroxytryptamine were investigated in pigeon retina whereas 5-hydroxytryptamine and somatostatin or glucagon... (More)

A method was developed for the simultaneous demonstration of two specific cellular constituents. Cryostat sections of formaldehyde fixed tissue from retinae treated with ((3)H)-5-hydroxytryptamine were subjected to immunohistochemistry and subsequent autoradiography. The method takes advantage of the very efficient and specific uptake mechanism that many types of neurons possess which makes it possible to label them with radioactivity. With this method a study was made on the possible co-occurrence in the avian retina of 5-hydroxytryptamine on one hand and somatostatin, glucagon and substance P on the other. Substance P and 5-hydroxytryptamine were investigated in pigeon retina whereas 5-hydroxytryptamine and somatostatin or glucagon were investigated in chicken retina. Though a large number of cell bodies were examined no co-occurrence of 5-hydroxytryptamine and any of the peptides was found. The sensitivity of the method allows an assertion that if present, double labelled neurons are likely to number less than 0.5-1% of the respective populations.

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type
Contribution to journal
publication status
published
subject
in
Neurochemistry International
volume
5
issue
5
pages
86 - 579
publisher
Elsevier
external identifiers
  • pmid:20487984
  • scopus:0020616125
ISSN
0197-0186
DOI
10.1016/0197-0186(83)90049-9
language
English
LU publication?
yes
id
a241ede2-7e7a-4a83-a207-6f562d7d7496
date added to LUP
2022-01-07 15:20:35
date last changed
2024-01-05 23:41:48
@article{a241ede2-7e7a-4a83-a207-6f562d7d7496,
  abstract     = {{<p>A method was developed for the simultaneous demonstration of two specific cellular constituents. Cryostat sections of formaldehyde fixed tissue from retinae treated with ((3)H)-5-hydroxytryptamine were subjected to immunohistochemistry and subsequent autoradiography. The method takes advantage of the very efficient and specific uptake mechanism that many types of neurons possess which makes it possible to label them with radioactivity. With this method a study was made on the possible co-occurrence in the avian retina of 5-hydroxytryptamine on one hand and somatostatin, glucagon and substance P on the other. Substance P and 5-hydroxytryptamine were investigated in pigeon retina whereas 5-hydroxytryptamine and somatostatin or glucagon were investigated in chicken retina. Though a large number of cell bodies were examined no co-occurrence of 5-hydroxytryptamine and any of the peptides was found. The sensitivity of the method allows an assertion that if present, double labelled neurons are likely to number less than 0.5-1% of the respective populations.</p>}},
  author       = {{Tornqvist, K and Ehinger, B}},
  issn         = {{0197-0186}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{86--579}},
  publisher    = {{Elsevier}},
  series       = {{Neurochemistry International}},
  title        = {{Simultaneous immunohistochemistry and autoradiography of peptides and 5-hydroxytryptamine in bird retina}},
  url          = {{http://dx.doi.org/10.1016/0197-0186(83)90049-9}},
  doi          = {{10.1016/0197-0186(83)90049-9}},
  volume       = {{5}},
  year         = {{1983}},
}