Influence of experimental errors on the determination of flux control coefficients from transient metabolic concentrations
(1996) In Biochemical Journal 313. p.721-727- Abstract
- The influence of experimental errors on the determination of flux control coefficients from transient metabolite concentrations with the method proposed by Delgado and Liao [(1992) Biochem. J. 282, 919-927] has been investigated by using Monte Carlo simulations. The method requires least-squares fitting of the transient metabolite concentrations. Three different fitting methods have been evaluated. Simulated metabolite concentrations of a fictive metabolic pathway were scattered randomly, emulating experimental errors, before performing the fits. This was repeated a large number of times; the mean values and standard deviations of the resulting control coefficients are reported. The results show that the proposed method for determining... (More)
- The influence of experimental errors on the determination of flux control coefficients from transient metabolite concentrations with the method proposed by Delgado and Liao [(1992) Biochem. J. 282, 919-927] has been investigated by using Monte Carlo simulations. The method requires least-squares fitting of the transient metabolite concentrations. Three different fitting methods have been evaluated. Simulated metabolite concentrations of a fictive metabolic pathway were scattered randomly, emulating experimental errors, before performing the fits. This was repeated a large number of times; the mean values and standard deviations of the resulting control coefficients are reported. The results show that the proposed method for determining control coefficients is too sensitive to experimental errors to be practicable, with theoretically justified fitting methods. This is in particular due to the high degree of correlation between the concentrations. An alternative ad hoc fitting method produced biased mean values of the estimates of the control coefficients, but with remarkably low standard deviations. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3911535
- author
- Ehlde, M and Zacchi, Guido LU
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochemical Journal
- volume
- 313
- pages
- 721 - 727
- publisher
- Portland Press
- external identifiers
-
- wos:A1996TU58500004
- scopus:0030068394
- ISSN
- 0264-6021
- language
- English
- LU publication?
- yes
- id
- a25240f3-949a-4eef-91b8-f5d0dd53a216 (old id 3911535)
- alternative location
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1216970/
- date added to LUP
- 2016-04-01 16:17:17
- date last changed
- 2023-09-04 15:58:20
@article{a25240f3-949a-4eef-91b8-f5d0dd53a216,
abstract = {{The influence of experimental errors on the determination of flux control coefficients from transient metabolite concentrations with the method proposed by Delgado and Liao [(1992) Biochem. J. 282, 919-927] has been investigated by using Monte Carlo simulations. The method requires least-squares fitting of the transient metabolite concentrations. Three different fitting methods have been evaluated. Simulated metabolite concentrations of a fictive metabolic pathway were scattered randomly, emulating experimental errors, before performing the fits. This was repeated a large number of times; the mean values and standard deviations of the resulting control coefficients are reported. The results show that the proposed method for determining control coefficients is too sensitive to experimental errors to be practicable, with theoretically justified fitting methods. This is in particular due to the high degree of correlation between the concentrations. An alternative ad hoc fitting method produced biased mean values of the estimates of the control coefficients, but with remarkably low standard deviations.}},
author = {{Ehlde, M and Zacchi, Guido}},
issn = {{0264-6021}},
language = {{eng}},
pages = {{721--727}},
publisher = {{Portland Press}},
series = {{Biochemical Journal}},
title = {{Influence of experimental errors on the determination of flux control coefficients from transient metabolic concentrations}},
url = {{http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1216970/}},
volume = {{313}},
year = {{1996}},
}