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Repopulation of adult and neonatal mice with human hepatocytes : A chimeric animal model

Bissig, Karl Dimiter; Le, Tam T.; Woods, Niels Bjarne LU and Verma, Inder M. (2007) In Proceedings of the National Academy of Sciences of the United States of America 104(51). p.20507-20511
Abstract

We report the successful transplantation of human hepatocytes in immunodeficient, fumarylacetoacetate hydrolase-deficient (fah-/-) mice. Engraftment occurs over the entire liver acinus upon transplantation. A few weeks after transplantation, increasing concentrations of human proteins (e.g., human albumin and human C3a) can be measured in the blood of the recipient mouse. No fusion between mouse and human hepatocytes can be detected. Three months after transplantation, up to 20% of the mouse liver is repopulated by human hepatocytes, and sustained expression of lentiviral vector transduced gene can be observed. We further report the development of a hepatocyte transplantation method involving a transcutaneous, intrahepatic... (More)

We report the successful transplantation of human hepatocytes in immunodeficient, fumarylacetoacetate hydrolase-deficient (fah-/-) mice. Engraftment occurs over the entire liver acinus upon transplantation. A few weeks after transplantation, increasing concentrations of human proteins (e.g., human albumin and human C3a) can be measured in the blood of the recipient mouse. No fusion between mouse and human hepatocytes can be detected. Three months after transplantation, up to 20% of the mouse liver is repopulated by human hepatocytes, and sustained expression of lentiviral vector transduced gene can be observed. We further report the development of a hepatocyte transplantation method involving a transcutaneous, intrahepatic injection in neonatal mice. Human hepatocytes engraft over the entire injected lobe with an expansion pattern similar to those observed with intrasplenic transplantation.

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author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Fumarylacetoacetate hydrolase, Hepatocyte transplantation, IL-2 receptor common gamma chain, Lentivirus, Recombination activating gene 2
in
Proceedings of the National Academy of Sciences of the United States of America
volume
104
issue
51
pages
20507 - 20511
publisher
National Acad Sciences
external identifiers
  • scopus:38049164210
ISSN
0027-8424
DOI
10.1073/pnas.0710528105
language
English
LU publication?
no
id
a25dc6bf-962a-4a75-a86c-578a2b5aa15a
date added to LUP
2017-01-26 08:24:30
date last changed
2017-11-12 04:28:25
@article{a25dc6bf-962a-4a75-a86c-578a2b5aa15a,
  abstract     = {<p>We report the successful transplantation of human hepatocytes in immunodeficient, fumarylacetoacetate hydrolase-deficient (fah<sup>-/-</sup>) mice. Engraftment occurs over the entire liver acinus upon transplantation. A few weeks after transplantation, increasing concentrations of human proteins (e.g., human albumin and human C3a) can be measured in the blood of the recipient mouse. No fusion between mouse and human hepatocytes can be detected. Three months after transplantation, up to 20% of the mouse liver is repopulated by human hepatocytes, and sustained expression of lentiviral vector transduced gene can be observed. We further report the development of a hepatocyte transplantation method involving a transcutaneous, intrahepatic injection in neonatal mice. Human hepatocytes engraft over the entire injected lobe with an expansion pattern similar to those observed with intrasplenic transplantation.</p>},
  author       = {Bissig, Karl Dimiter and Le, Tam T. and Woods, Niels Bjarne and Verma, Inder M.},
  issn         = {0027-8424},
  keyword      = {Fumarylacetoacetate hydrolase,Hepatocyte transplantation,IL-2 receptor common gamma chain,Lentivirus,Recombination activating gene 2},
  language     = {eng},
  month        = {12},
  number       = {51},
  pages        = {20507--20511},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences of the United States of America},
  title        = {Repopulation of adult and neonatal mice with human hepatocytes : A chimeric animal model},
  url          = {http://dx.doi.org/10.1073/pnas.0710528105},
  volume       = {104},
  year         = {2007},
}