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Chapter 6. Qualitative and quantitative assessment of the activity of the yeast nonsense-mediated mRNA decay pathway

He, Feng ; Amrani, Nadia ; Johansson, Marcus J O LU and Jacobson, Allan (2008) In Methods in Enzymology 449. p.47-127
Abstract

The yeast Saccharomyces cerevisiae provides an ideal model system for elucidation of the molecular mechanisms that regulate the nonsense-mediated mRNA decay (NMD) pathway. This chapter describes an array of molecular biological, genetic, and biochemical tools that facilitate the characterization of transcripts that comprise NMD substrates and provide insights into the roles of the upf/nmd proteins in mRNA decay and translation termination. Examples illustrate the use of these methods in wild-type and NMD-deficient cells to monitor the abundance, structure, and half-lives of nonsense-containing mRNAs, the read through of premature termination codons by the ribosome, and the positioning of ribosomes at or near normal and premature... (More)

The yeast Saccharomyces cerevisiae provides an ideal model system for elucidation of the molecular mechanisms that regulate the nonsense-mediated mRNA decay (NMD) pathway. This chapter describes an array of molecular biological, genetic, and biochemical tools that facilitate the characterization of transcripts that comprise NMD substrates and provide insights into the roles of the upf/nmd proteins in mRNA decay and translation termination. Examples illustrate the use of these methods in wild-type and NMD-deficient cells to monitor the abundance, structure, and half-lives of nonsense-containing mRNAs, the read through of premature termination codons by the ribosome, and the positioning of ribosomes at or near normal and premature termination codons.

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Please use this url to cite or link to this publication:
author
; ; and
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
keywords
Codon, Nonsense/genetics, RNA Stability/genetics, Saccharomyces cerevisiae/genetics, Signal Transduction/genetics
host publication
RNA Turnover in Eukaryotes : Analysis of Specialized and Quality Control RNA Decay Pathways - Analysis of Specialized and Quality Control RNA Decay Pathways
series title
Methods in Enzymology
volume
449
pages
47 - 127
external identifiers
  • scopus:59649110288
  • pmid:19215756
ISSN
0076-6879
ISBN
978-0-12-374584-2
DOI
10.1016/S0076-6879(08)02406-3
language
English
LU publication?
no
id
a25e8886-3a9f-41c2-9c9a-f2e483041772
date added to LUP
2024-03-05 16:47:32
date last changed
2025-07-13 16:50:21
@inbook{a25e8886-3a9f-41c2-9c9a-f2e483041772,
  abstract     = {{<p>The yeast Saccharomyces cerevisiae provides an ideal model system for elucidation of the molecular mechanisms that regulate the nonsense-mediated mRNA decay (NMD) pathway. This chapter describes an array of molecular biological, genetic, and biochemical tools that facilitate the characterization of transcripts that comprise NMD substrates and provide insights into the roles of the upf/nmd proteins in mRNA decay and translation termination. Examples illustrate the use of these methods in wild-type and NMD-deficient cells to monitor the abundance, structure, and half-lives of nonsense-containing mRNAs, the read through of premature termination codons by the ribosome, and the positioning of ribosomes at or near normal and premature termination codons.</p>}},
  author       = {{He, Feng and Amrani, Nadia and Johansson, Marcus J O and Jacobson, Allan}},
  booktitle    = {{RNA Turnover in Eukaryotes : Analysis of Specialized and Quality Control RNA Decay Pathways}},
  isbn         = {{978-0-12-374584-2}},
  issn         = {{0076-6879}},
  keywords     = {{Codon, Nonsense/genetics; RNA Stability/genetics; Saccharomyces cerevisiae/genetics; Signal Transduction/genetics}},
  language     = {{eng}},
  pages        = {{47--127}},
  series       = {{Methods in Enzymology}},
  title        = {{Chapter 6. Qualitative and quantitative assessment of the activity of the yeast nonsense-mediated mRNA decay pathway}},
  url          = {{http://dx.doi.org/10.1016/S0076-6879(08)02406-3}},
  doi          = {{10.1016/S0076-6879(08)02406-3}},
  volume       = {{449}},
  year         = {{2008}},
}