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In-hospital metabolite changes in infective endocarditis—a longitudinal 1H NMR-based study

Klein, Christine Falk; Holle, Sarah Louise Kjølhede; Andersen, Malene Højgaard; Pedersen, Anders; Bundgaard, Henning; Iversen, Kasper Karmark and Malmendal, Anders LU (2019) In European Journal of Clinical Microbiology and Infectious Diseases
Abstract

Treatment of infective endocarditis (IE) is a 4–6-week provided course of intravenously administered antibiotics. The aim of this study was to investigate how serum metabolites as measured by proton nuclear magnetic resonance (1H NMR) spectroscopy are changing over time during the active phase of IE, and to see whether these metabolite changes might be used to monitor recovery in these patients. Patients hospitalized with first-time IE at Herlev Hospital, Denmark, from September 2015 to June 2017 were included. Longitudinal blood sampling was performed and serum was analyzed using 1H NMR. Orthogonal projection to latent structures discriminant analysis (OPLS-DA) was used to separate sample groups and analyze... (More)

Treatment of infective endocarditis (IE) is a 4–6-week provided course of intravenously administered antibiotics. The aim of this study was to investigate how serum metabolites as measured by proton nuclear magnetic resonance (1H NMR) spectroscopy are changing over time during the active phase of IE, and to see whether these metabolite changes might be used to monitor recovery in these patients. Patients hospitalized with first-time IE at Herlev Hospital, Denmark, from September 2015 to June 2017 were included. Longitudinal blood sampling was performed and serum was analyzed using 1H NMR. Orthogonal projection to latent structures discriminant analysis (OPLS-DA) was used to separate sample groups and analyze differences in metabolite profiles. Thirteen patients were included in the study (77% men, median age 62 years (IQR 53–77)). All patients were cured during the hospitalization without any relapse during 6 months of follow-up. We analyzed 61 serum samples (median 5 samples, range 2–8 per person) drawn in the treatment period after IE diagnosis. The main changes during the in-hospital period were decreased levels of glucose, mannose, leucine, isoleucine, phenylalanine, tyrosine, and signals from polyols and N-acetylated protein. The metabolomic changes could in contrast to the routinely used parameters CRP and leucocyte levels distinguish between the early and late stages of disease treatment. We present the first longitudinal study of 1H NMR metabolomics in patients with infective endocarditis. The metabolomic changes show a promising strength compared to routinely used clinical parameters.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Antibiotic treatment, Infective endocarditis, Longitudinal study, Metabolomics, NMR
in
European Journal of Clinical Microbiology and Infectious Diseases
publisher
Springer
external identifiers
  • scopus:85066910070
ISSN
0934-9723
DOI
10.1007/s10096-019-03586-z
language
English
LU publication?
yes
id
a29f7d3a-46f0-42cc-8ad4-014137928996
date added to LUP
2019-06-25 12:58:24
date last changed
2019-07-16 04:12:29
@article{a29f7d3a-46f0-42cc-8ad4-014137928996,
  abstract     = {<p>Treatment of infective endocarditis (IE) is a 4–6-week provided course of intravenously administered antibiotics. The aim of this study was to investigate how serum metabolites as measured by proton nuclear magnetic resonance (<sup>1</sup>H NMR) spectroscopy are changing over time during the active phase of IE, and to see whether these metabolite changes might be used to monitor recovery in these patients. Patients hospitalized with first-time IE at Herlev Hospital, Denmark, from September 2015 to June 2017 were included. Longitudinal blood sampling was performed and serum was analyzed using <sup>1</sup>H NMR. Orthogonal projection to latent structures discriminant analysis (OPLS-DA) was used to separate sample groups and analyze differences in metabolite profiles. Thirteen patients were included in the study (77% men, median age 62 years (IQR 53–77)). All patients were cured during the hospitalization without any relapse during 6 months of follow-up. We analyzed 61 serum samples (median 5 samples, range 2–8 per person) drawn in the treatment period after IE diagnosis. The main changes during the in-hospital period were decreased levels of glucose, mannose, leucine, isoleucine, phenylalanine, tyrosine, and signals from polyols and N-acetylated protein. The metabolomic changes could in contrast to the routinely used parameters CRP and leucocyte levels distinguish between the early and late stages of disease treatment. We present the first longitudinal study of <sup>1</sup>H NMR metabolomics in patients with infective endocarditis. The metabolomic changes show a promising strength compared to routinely used clinical parameters.</p>},
  author       = {Klein, Christine Falk and Holle, Sarah Louise Kjølhede and Andersen, Malene Højgaard and Pedersen, Anders and Bundgaard, Henning and Iversen, Kasper Karmark and Malmendal, Anders},
  issn         = {0934-9723},
  keyword      = {Antibiotic treatment,Infective endocarditis,Longitudinal study,Metabolomics,NMR},
  language     = {eng},
  month        = {05},
  publisher    = {Springer},
  series       = {European Journal of Clinical Microbiology and Infectious Diseases},
  title        = {In-hospital metabolite changes in infective endocarditis—a longitudinal <sup>1</sup>H NMR-based study},
  url          = {http://dx.doi.org/10.1007/s10096-019-03586-z},
  year         = {2019},
}