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Characterization of Relaxant Responses to Natriuretic Peptides in the Human Microcirculation In Vitro and In Vivo

Edvinsson, Marie-Louise LU ; Ahnstedt, Hilda LU ; Edvinsson, Lars LU and Andersson, Sven E. LU (2016) In Microcirculation 23(6). p.438-446
Abstract

Objective: We characterized the vasodilatory effects of ANP, BNP, and CNP in human subcutaneous arterioles in vitro and the cutaneous microcirculation in vivo. Methods: The in vitro experiments were performed using wire myography and the responses were characterized by the use of inhibitors for nitric oxide (L-NAME), prostaglandin synthesis (indomethacin), or the endothelium-derived hyperpolarization factor. In vivo, the vasorelaxant effect of iontophoretically administrated BNP or CNP was measured with a noninvasive laser Doppler technique. Involvement of nitric oxide or prostaglandins was assessed by L-NAME or indomethacin given by iontophoresis. Results: In vitro all three peptides showed significant vasodilatation with the efficacy... (More)

Objective: We characterized the vasodilatory effects of ANP, BNP, and CNP in human subcutaneous arterioles in vitro and the cutaneous microcirculation in vivo. Methods: The in vitro experiments were performed using wire myography and the responses were characterized by the use of inhibitors for nitric oxide (L-NAME), prostaglandin synthesis (indomethacin), or the endothelium-derived hyperpolarization factor. In vivo, the vasorelaxant effect of iontophoretically administrated BNP or CNP was measured with a noninvasive laser Doppler technique. Involvement of nitric oxide or prostaglandins was assessed by L-NAME or indomethacin given by iontophoresis. Results: In vitro all three peptides showed significant vasodilatation with the efficacy order: CNP > BNP = ANP. The BNP-induced vasodilatation, but not that of ANP or CNP, was significantly reduced by pretreatment with indomethacin or L-NAME. In vivo administration of BNP induced a marked vasodilatory response that was attenuated by local pretreatment of L-NAME. Indomethacin by itself resulted in increased cutaneous perfusion. Conclusions: NPs are potent vasodilators in the human subcutaneous circulation. The response to BNP differs from that of the other peptides as it seems dependent on cyclooxygenase products and nitric oxide.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
atrial natriuretic peptide, brain natriuretic peptide, c-type natriuretic peptide, human subcutaneous artery, vasodilation
in
Microcirculation
volume
23
issue
6
pages
9 pages
publisher
Taylor & Francis
external identifiers
  • scopus:84981747412
  • wos:000382834400005
ISSN
1073-9688
DOI
10.1111/micc.12290
language
English
LU publication?
yes
id
a356c3c0-aea5-4ef9-9192-23bd8b1966f7
date added to LUP
2016-09-20 17:17:28
date last changed
2017-01-01 08:34:28
@article{a356c3c0-aea5-4ef9-9192-23bd8b1966f7,
  abstract     = {<p>Objective: We characterized the vasodilatory effects of ANP, BNP, and CNP in human subcutaneous arterioles in vitro and the cutaneous microcirculation in vivo. Methods: The in vitro experiments were performed using wire myography and the responses were characterized by the use of inhibitors for nitric oxide (L-NAME), prostaglandin synthesis (indomethacin), or the endothelium-derived hyperpolarization factor. In vivo, the vasorelaxant effect of iontophoretically administrated BNP or CNP was measured with a noninvasive laser Doppler technique. Involvement of nitric oxide or prostaglandins was assessed by L-NAME or indomethacin given by iontophoresis. Results: In vitro all three peptides showed significant vasodilatation with the efficacy order: CNP &gt; BNP = ANP. The BNP-induced vasodilatation, but not that of ANP or CNP, was significantly reduced by pretreatment with indomethacin or L-NAME. In vivo administration of BNP induced a marked vasodilatory response that was attenuated by local pretreatment of L-NAME. Indomethacin by itself resulted in increased cutaneous perfusion. Conclusions: NPs are potent vasodilators in the human subcutaneous circulation. The response to BNP differs from that of the other peptides as it seems dependent on cyclooxygenase products and nitric oxide.</p>},
  author       = {Edvinsson, Marie-Louise and Ahnstedt, Hilda and Edvinsson, Lars and Andersson, Sven E.},
  issn         = {1073-9688},
  keyword      = {atrial natriuretic peptide,brain natriuretic peptide,c-type natriuretic peptide,human subcutaneous artery,vasodilation},
  language     = {eng},
  month        = {08},
  number       = {6},
  pages        = {438--446},
  publisher    = {Taylor & Francis},
  series       = {Microcirculation},
  title        = {Characterization of Relaxant Responses to Natriuretic Peptides in the Human Microcirculation In Vitro and In Vivo},
  url          = {http://dx.doi.org/10.1111/micc.12290},
  volume       = {23},
  year         = {2016},
}