Surface characterization of the Gram-positive bacteria Bacillus subtilis - an XPS study
(2006) In Surface and Interface Analysis 38. p.202-205- Abstract
- Interactions at the bacteria-solid interface in aquatic environments are of crucial importance in biofilm formation processes. The characterization of microbial surfaces properties is therefore a very important step for the understanding of these interactions. In this study, XPS spectra of Bacillus subtilis wet pastes centrifuged from suspensions over a span of pH values were recorded using a fast-freezing procedure. The chemical composition of the bacterial surface was elucidated and the presence of surface sites containing carboxylate, phosphate and amine functional groups was established. It was possible to distinguish between charged and uncharged amine sites on the surface and follow their deprotonation reactions as a function of pH.... (More)
- Interactions at the bacteria-solid interface in aquatic environments are of crucial importance in biofilm formation processes. The characterization of microbial surfaces properties is therefore a very important step for the understanding of these interactions. In this study, XPS spectra of Bacillus subtilis wet pastes centrifuged from suspensions over a span of pH values were recorded using a fast-freezing procedure. The chemical composition of the bacterial surface was elucidated and the presence of surface sites containing carboxylate, phosphate and amine functional groups was established. It was possible to distinguish between charged and uncharged amine sites on the surface and follow their deprotonation reactions as a function of pH. It is suggested that increased metabolic activity around the physiological pH results in the excretion of proteins and/or peptides which are possibly related to the production of extracellular polymeric substances (EPS). Copyright (C) 2006 John Wiley & Sons, Ltd. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4332480
- author
- Leone, L. ; Loring, J. ; Sjooberg, S. ; Persson, Per LU and Shchukarev, A.
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Surface and Interface Analysis
- volume
- 38
- pages
- 202 - 205
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:33646677484
- ISSN
- 0142-2421
- DOI
- 10.1002/sia.2184
- language
- English
- LU publication?
- no
- additional info
- 4
- id
- a37e7497-e401-4fa3-af94-c854f2978fee (old id 4332480)
- date added to LUP
- 2016-04-01 16:27:21
- date last changed
- 2022-03-30 08:03:33
@article{a37e7497-e401-4fa3-af94-c854f2978fee,
abstract = {{Interactions at the bacteria-solid interface in aquatic environments are of crucial importance in biofilm formation processes. The characterization of microbial surfaces properties is therefore a very important step for the understanding of these interactions. In this study, XPS spectra of Bacillus subtilis wet pastes centrifuged from suspensions over a span of pH values were recorded using a fast-freezing procedure. The chemical composition of the bacterial surface was elucidated and the presence of surface sites containing carboxylate, phosphate and amine functional groups was established. It was possible to distinguish between charged and uncharged amine sites on the surface and follow their deprotonation reactions as a function of pH. It is suggested that increased metabolic activity around the physiological pH results in the excretion of proteins and/or peptides which are possibly related to the production of extracellular polymeric substances (EPS). Copyright (C) 2006 John Wiley & Sons, Ltd.}},
author = {{Leone, L. and Loring, J. and Sjooberg, S. and Persson, Per and Shchukarev, A.}},
issn = {{0142-2421}},
language = {{eng}},
pages = {{202--205}},
publisher = {{Wiley-Blackwell}},
series = {{Surface and Interface Analysis}},
title = {{Surface characterization of the Gram-positive bacteria Bacillus subtilis - an XPS study}},
url = {{http://dx.doi.org/10.1002/sia.2184}},
doi = {{10.1002/sia.2184}},
volume = {{38}},
year = {{2006}},
}