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Hepatocytes and IL-15 : A favorable microenvironment for T cell survival and CD8+ T cell differentiation

Correia, Margareta P.; Cardoso, Elsa M.; Pereira, Carlos F. LU ; Neves, Rui; Uhrberg, Markus and Arosa, Fernando A. (2009) In Journal of Immunology 182(10). p.6149-6159
Abstract

Human intrahepatic lymphocytes are enriched in CD1d-unrestricted T cells coexpressing NKR. Although the origin of this population remains controversial, it is possible to speculate that the hepatic microenvironment, namely epithelial cells or the cytokine milieu, may play a role in its shaping. IL-15 is constitutively expressed in the liver and has a key role in activation and survival of innate and tissue-associated immune cells. In this in vitro study, we examined whether hepatocyte cell lines and/or IL-15 could play a role in the generation of NK-like T cells. The results show that both HepG2 cells and a human immortalized hepatocyte cell line increase survival and drive basal proliferation of T cells. In addition, IL-15 was capable... (More)

Human intrahepatic lymphocytes are enriched in CD1d-unrestricted T cells coexpressing NKR. Although the origin of this population remains controversial, it is possible to speculate that the hepatic microenvironment, namely epithelial cells or the cytokine milieu, may play a role in its shaping. IL-15 is constitutively expressed in the liver and has a key role in activation and survival of innate and tissue-associated immune cells. In this in vitro study, we examined whether hepatocyte cell lines and/or IL-15 could play a role in the generation of NK-like T cells. The results show that both HepG2 cells and a human immortalized hepatocyte cell line increase survival and drive basal proliferation of T cells. In addition, IL-15 was capable of inducing Ag-independent up-regulation of NKR, including NKG2A, Ig-like receptors, and de novo expression of CD56 and NKp46 in CD8+CD56- T cells. In conclusion, our study suggests that hepatocytes and IL-15 create a favorable microenvironment for T cells to growth and survive. It can be proposed that the increased percentage of intrahepatic nonclassical NKT cells could be in part due to a local CD8+ T cell differentiation.

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author
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published
in
Journal of Immunology
volume
182
issue
10
pages
11 pages
publisher
American Association of Immunologists
external identifiers
  • scopus:77952369069
ISSN
0022-1767
DOI
10.4049/jimmunol.0802470
language
English
LU publication?
no
id
a3c0a154-844e-47b5-8d28-8466a9a407dc
date added to LUP
2017-10-02 19:29:45
date last changed
2017-10-12 13:51:31
@article{a3c0a154-844e-47b5-8d28-8466a9a407dc,
  abstract     = {<p>Human intrahepatic lymphocytes are enriched in CD1d-unrestricted T cells coexpressing NKR. Although the origin of this population remains controversial, it is possible to speculate that the hepatic microenvironment, namely epithelial cells or the cytokine milieu, may play a role in its shaping. IL-15 is constitutively expressed in the liver and has a key role in activation and survival of innate and tissue-associated immune cells. In this in vitro study, we examined whether hepatocyte cell lines and/or IL-15 could play a role in the generation of NK-like T cells. The results show that both HepG2 cells and a human immortalized hepatocyte cell line increase survival and drive basal proliferation of T cells. In addition, IL-15 was capable of inducing Ag-independent up-regulation of NKR, including NKG2A, Ig-like receptors, and de novo expression of CD56 and NKp46 in CD8<sup>+</sup>CD56<sup>-</sup> T cells. In conclusion, our study suggests that hepatocytes and IL-15 create a favorable microenvironment for T cells to growth and survive. It can be proposed that the increased percentage of intrahepatic nonclassical NKT cells could be in part due to a local CD8<sup>+</sup> T cell differentiation.</p>},
  author       = {Correia, Margareta P. and Cardoso, Elsa M. and Pereira, Carlos F. and Neves, Rui and Uhrberg, Markus and Arosa, Fernando A.},
  issn         = {0022-1767},
  language     = {eng},
  month        = {05},
  number       = {10},
  pages        = {6149--6159},
  publisher    = {American Association of Immunologists},
  series       = {Journal of Immunology},
  title        = {Hepatocytes and IL-15 : A favorable microenvironment for T cell survival and CD8+ T cell differentiation},
  url          = {http://dx.doi.org/10.4049/jimmunol.0802470},
  volume       = {182},
  year         = {2009},
}