Hepatocytes and IL-15 : A favorable microenvironment for T cell survival and CD8+ T cell differentiation
(2009) In Journal of Immunology 182(10). p.6149-6159- Abstract
Human intrahepatic lymphocytes are enriched in CD1d-unrestricted T cells coexpressing NKR. Although the origin of this population remains controversial, it is possible to speculate that the hepatic microenvironment, namely epithelial cells or the cytokine milieu, may play a role in its shaping. IL-15 is constitutively expressed in the liver and has a key role in activation and survival of innate and tissue-associated immune cells. In this in vitro study, we examined whether hepatocyte cell lines and/or IL-15 could play a role in the generation of NK-like T cells. The results show that both HepG2 cells and a human immortalized hepatocyte cell line increase survival and drive basal proliferation of T cells. In addition, IL-15 was capable... (More)
Human intrahepatic lymphocytes are enriched in CD1d-unrestricted T cells coexpressing NKR. Although the origin of this population remains controversial, it is possible to speculate that the hepatic microenvironment, namely epithelial cells or the cytokine milieu, may play a role in its shaping. IL-15 is constitutively expressed in the liver and has a key role in activation and survival of innate and tissue-associated immune cells. In this in vitro study, we examined whether hepatocyte cell lines and/or IL-15 could play a role in the generation of NK-like T cells. The results show that both HepG2 cells and a human immortalized hepatocyte cell line increase survival and drive basal proliferation of T cells. In addition, IL-15 was capable of inducing Ag-independent up-regulation of NKR, including NKG2A, Ig-like receptors, and de novo expression of CD56 and NKp46 in CD8+CD56- T cells. In conclusion, our study suggests that hepatocytes and IL-15 create a favorable microenvironment for T cells to growth and survive. It can be proposed that the increased percentage of intrahepatic nonclassical NKT cells could be in part due to a local CD8+ T cell differentiation.
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- author
- Correia, Margareta P. ; Cardoso, Elsa M. ; Pereira, Carlos F. LU ; Neves, Rui ; Uhrberg, Markus and Arosa, Fernando A.
- publishing date
- 2009-05-15
- type
- Contribution to journal
- publication status
- published
- in
- Journal of Immunology
- volume
- 182
- issue
- 10
- pages
- 11 pages
- publisher
- American Association of Immunologists
- external identifiers
-
- pmid:19414768
- scopus:77952369069
- ISSN
- 0022-1767
- DOI
- 10.4049/jimmunol.0802470
- language
- English
- LU publication?
- no
- id
- a3c0a154-844e-47b5-8d28-8466a9a407dc
- date added to LUP
- 2017-10-02 19:29:45
- date last changed
- 2024-08-05 05:31:08
@article{a3c0a154-844e-47b5-8d28-8466a9a407dc, abstract = {{<p>Human intrahepatic lymphocytes are enriched in CD1d-unrestricted T cells coexpressing NKR. Although the origin of this population remains controversial, it is possible to speculate that the hepatic microenvironment, namely epithelial cells or the cytokine milieu, may play a role in its shaping. IL-15 is constitutively expressed in the liver and has a key role in activation and survival of innate and tissue-associated immune cells. In this in vitro study, we examined whether hepatocyte cell lines and/or IL-15 could play a role in the generation of NK-like T cells. The results show that both HepG2 cells and a human immortalized hepatocyte cell line increase survival and drive basal proliferation of T cells. In addition, IL-15 was capable of inducing Ag-independent up-regulation of NKR, including NKG2A, Ig-like receptors, and de novo expression of CD56 and NKp46 in CD8<sup>+</sup>CD56<sup>-</sup> T cells. In conclusion, our study suggests that hepatocytes and IL-15 create a favorable microenvironment for T cells to growth and survive. It can be proposed that the increased percentage of intrahepatic nonclassical NKT cells could be in part due to a local CD8<sup>+</sup> T cell differentiation.</p>}}, author = {{Correia, Margareta P. and Cardoso, Elsa M. and Pereira, Carlos F. and Neves, Rui and Uhrberg, Markus and Arosa, Fernando A.}}, issn = {{0022-1767}}, language = {{eng}}, month = {{05}}, number = {{10}}, pages = {{6149--6159}}, publisher = {{American Association of Immunologists}}, series = {{Journal of Immunology}}, title = {{Hepatocytes and IL-15 : A favorable microenvironment for T cell survival and CD8+ T cell differentiation}}, url = {{http://dx.doi.org/10.4049/jimmunol.0802470}}, doi = {{10.4049/jimmunol.0802470}}, volume = {{182}}, year = {{2009}}, }