Optical visualization of Alzheimer's pathology via multiphoton-excited intrinsic fluorescence and second harmonic generation
Kwan, Alex C. ; Duff, Karen ; Gouras, Gunnar K. LU
and Webb, Watt W.
(2009)
In Optics Express
17(5).
p.3679-3689
- Abstract
Intrinsic optical emissions, such as autofluorescence and second harmonic generation (SHG), are potentially useful for functional fluorescence imaging and biomedical disease diagnosis for neurodegenerative diseases such as Alzheimer's disease (AD). Here, using multiphoton and SHG microscopy, we identified sources of intrinsic emissions in ex vivo, acute brain slices from AD transgenic mouse models. We observed autofluorescence and SHG at senile plaques as well as characterized their emission spectra. The utility of intrinsic emissions was demonstrated by imaging senile plaque autofluorescence in conjunction with SHG from microtubule arrays to assess the polarity of microtubules near pathological lesions. Our results suggest that tissues... (More)
Intrinsic optical emissions, such as autofluorescence and second harmonic generation (SHG), are potentially useful for functional fluorescence imaging and biomedical disease diagnosis for neurodegenerative diseases such as Alzheimer's disease (AD). Here, using multiphoton and SHG microscopy, we identified sources of intrinsic emissions in ex vivo, acute brain slices from AD transgenic mouse models. We observed autofluorescence and SHG at senile plaques as well as characterized their emission spectra. The utility of intrinsic emissions was demonstrated by imaging senile plaque autofluorescence in conjunction with SHG from microtubule arrays to assess the polarity of microtubules near pathological lesions. Our results suggest that tissues from AD transgenic models contain distinct intrinsic emissions, which can provide valuable information about the disease mechanisms.
(Less)
- author
- Kwan, Alex C.
; Duff, Karen
; Gouras, Gunnar K.
LU
and Webb, Watt W.
- publishing date
- 2009-03-02
- type
- Contribution to journal
- publication status
- published
- in
- Optics Express
- volume
- 17
- issue
- 5
- pages
- 11 pages
- publisher
- Optical Society of America
- external identifiers
-
- scopus:61549089382
- pmid:19259208
- ISSN
- 1094-4087
- DOI
- 10.1364/OE.17.003679
- language
- English
- LU publication?
- no
- id
- a3d7e166-f5ed-4b9e-91ba-b4ebb01450da
- date added to LUP
- 2020-02-20 14:24:21
- date last changed
- 2024-04-03 03:09:09
@article{a3d7e166-f5ed-4b9e-91ba-b4ebb01450da,
abstract = {{<p>Intrinsic optical emissions, such as autofluorescence and second harmonic generation (SHG), are potentially useful for functional fluorescence imaging and biomedical disease diagnosis for neurodegenerative diseases such as Alzheimer's disease (AD). Here, using multiphoton and SHG microscopy, we identified sources of intrinsic emissions in ex vivo, acute brain slices from AD transgenic mouse models. We observed autofluorescence and SHG at senile plaques as well as characterized their emission spectra. The utility of intrinsic emissions was demonstrated by imaging senile plaque autofluorescence in conjunction with SHG from microtubule arrays to assess the polarity of microtubules near pathological lesions. Our results suggest that tissues from AD transgenic models contain distinct intrinsic emissions, which can provide valuable information about the disease mechanisms.</p>}},
author = {{Kwan, Alex C. and Duff, Karen and Gouras, Gunnar K. and Webb, Watt W.}},
issn = {{1094-4087}},
language = {{eng}},
month = {{03}},
number = {{5}},
pages = {{3679--3689}},
publisher = {{Optical Society of America}},
series = {{Optics Express}},
title = {{Optical visualization of Alzheimer's pathology via multiphoton-excited intrinsic fluorescence and second harmonic generation}},
url = {{http://dx.doi.org/10.1364/OE.17.003679}},
doi = {{10.1364/OE.17.003679}},
volume = {{17}},
year = {{2009}},
}