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Whole-mount in situ hybridization and immunohistochemistry in Xenopus embryos.

Pera, Edgar LU ; Acosta, Helena LU ; Gouignard, Nadège LU and Climent, Maria LU (2015) In Neuromethods 99. p.151-167
Abstract
Xenopus is a favorable experimental model in developmental biology. With its fast and external development, high number of progeny and large size, early embryos are well suited for micromanipulation to study the function of genes with relevance to human diseases. In this chapter, we present a combined method for lineage tracing and whole-mount in situ hybridization. In addition, we present protocols for immunohistochemistry and assays to monitor the cell proliferation and apoptosis in whole embryos.
Abstract (Swedish)
Xenopus is a favorable experimental model in developmental biology. With its fast and external development, high number of progeny and large size, early embryos are well suited for micromanipulation to study the function of genes with relevance to human diseases. In this chapter, we present a combined method for lineage tracing and whole-mount in situ hybridization. In addition, we present protocols for
immunohistochemistry and assays to monitor the cell proliferation and apoptosis in whole embryos.
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Xenopus , Whole-mount in situ hybridization , Immunohistochemistry , pH3 , TUNEL assay
host publication
In Situ Hybridization Methods
series title
Neuromethods
editor
Hauptmannn, Giselbert
volume
99
edition
1
pages
151 - 167
publisher
Humana Press
ISSN
0893-2336
1940-6045
ISBN
978-1-4939-2303-8
978-1-4939-2302-1
978-1-4939-4463-7
DOI
10.1007/978-1-4939-2303-8
language
English
LU publication?
yes
id
a3fa4a6f-406a-425c-ba82-b20dfb1be436
date added to LUP
2018-02-19 17:03:01
date last changed
2018-11-21 21:38:05
@inbook{a3fa4a6f-406a-425c-ba82-b20dfb1be436,
  abstract     = {{Xenopus is a favorable experimental model in developmental biology. With its fast and external development, high number of progeny and large size, early embryos are well suited for micromanipulation to study the function of genes with relevance to human diseases. In this chapter, we present a combined method for lineage tracing and whole-mount in situ hybridization. In addition, we present protocols for immunohistochemistry and assays to monitor the cell proliferation and apoptosis in whole embryos.}},
  author       = {{Pera, Edgar and Acosta, Helena and Gouignard, Nadège and Climent, Maria}},
  booktitle    = {{In Situ Hybridization Methods}},
  editor       = {{Hauptmannn, Giselbert}},
  isbn         = {{978-1-4939-2303-8}},
  issn         = {{0893-2336}},
  keywords     = {{Xenopus , Whole-mount in situ hybridization , Immunohistochemistry , pH3 , TUNEL assay}},
  language     = {{eng}},
  pages        = {{151--167}},
  publisher    = {{Humana Press}},
  series       = {{Neuromethods}},
  title        = {{Whole-mount in situ hybridization and immunohistochemistry in Xenopus embryos.}},
  url          = {{http://dx.doi.org/10.1007/978-1-4939-2303-8}},
  doi          = {{10.1007/978-1-4939-2303-8}},
  volume       = {{99}},
  year         = {{2015}},
}