Conformations of the regulatory domain of cardiac troponin C examined by residual dipolar couplings
(2000) In European Journal of Biochemistry 267(22). p.6665-6672- Abstract
Conformations of the regulatory domain of cardiac troponin C (cNTnC) were studied by means of residual dipolar couplings measured from samples dissolved in dilute liquid crystals. Changes in the main chain HN residual dipolar couplings revealed a conformational change in cNTnC due to the complexation with the second binding region (amino acids 148-163) of cardiac troponin I (cTnI). Formation of the complex is accompanied with a molecular realignment in the liquid crystal. The residual dipolar couplings measured for apo-cNTnC and the complex with TnI were in agreement with the values computed from the corresponding closed and open solution structures, whereas for the calcium-loaded conformation the correlation and quality factor were... (More)
Conformations of the regulatory domain of cardiac troponin C (cNTnC) were studied by means of residual dipolar couplings measured from samples dissolved in dilute liquid crystals. Changes in the main chain HN residual dipolar couplings revealed a conformational change in cNTnC due to the complexation with the second binding region (amino acids 148-163) of cardiac troponin I (cTnI). Formation of the complex is accompanied with a molecular realignment in the liquid crystal. The residual dipolar couplings measured for apo-cNTnC and the complex with TnI were in agreement with the values computed from the corresponding closed and open solution structures, whereas for the calcium-loaded conformation the correlation and quality factor were only modest. Ca2+-cNTnC may be subject to conformational exchange. The data support the model that cardiac troponin C functions as a calcium-dependent open-closed switch, such as the skeletal troponin C.
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- author
- Pääkkönen, Kimmo ; Sorsa, Tia ; Drakenberg, Torbjörn LU ; Pollesello, Piero ; Tilgmann, Carola LU ; Permi, Perttu ; Heikkinen, Sami ; Kilpeläinen, Ilkka and Annila, Arto
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- keywords
- Calcium-dependent binding, Cardiac troponin, Conformational exchange, NMR, Residual dipolar couplings
- in
- European Journal of Biochemistry
- volume
- 267
- issue
- 22
- pages
- 8 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0033773598
- pmid:11054120
- ISSN
- 0014-2956
- DOI
- 10.1046/j.1432-1327.2000.01763.x
- language
- English
- LU publication?
- no
- id
- a42bde40-b6a6-4ed9-9843-5d229af63b8b
- date added to LUP
- 2016-04-11 13:16:00
- date last changed
- 2024-04-18 21:32:52
@article{a42bde40-b6a6-4ed9-9843-5d229af63b8b, abstract = {{<p>Conformations of the regulatory domain of cardiac troponin C (cNTnC) were studied by means of residual dipolar couplings measured from samples dissolved in dilute liquid crystals. Changes in the main chain HN residual dipolar couplings revealed a conformational change in cNTnC due to the complexation with the second binding region (amino acids 148-163) of cardiac troponin I (cTnI). Formation of the complex is accompanied with a molecular realignment in the liquid crystal. The residual dipolar couplings measured for apo-cNTnC and the complex with TnI were in agreement with the values computed from the corresponding closed and open solution structures, whereas for the calcium-loaded conformation the correlation and quality factor were only modest. Ca<sup>2+</sup>-cNTnC may be subject to conformational exchange. The data support the model that cardiac troponin C functions as a calcium-dependent open-closed switch, such as the skeletal troponin C.</p>}}, author = {{Pääkkönen, Kimmo and Sorsa, Tia and Drakenberg, Torbjörn and Pollesello, Piero and Tilgmann, Carola and Permi, Perttu and Heikkinen, Sami and Kilpeläinen, Ilkka and Annila, Arto}}, issn = {{0014-2956}}, keywords = {{Calcium-dependent binding; Cardiac troponin; Conformational exchange; NMR; Residual dipolar couplings}}, language = {{eng}}, number = {{22}}, pages = {{6665--6672}}, publisher = {{Wiley-Blackwell}}, series = {{European Journal of Biochemistry}}, title = {{Conformations of the regulatory domain of cardiac troponin C examined by residual dipolar couplings}}, url = {{http://dx.doi.org/10.1046/j.1432-1327.2000.01763.x}}, doi = {{10.1046/j.1432-1327.2000.01763.x}}, volume = {{267}}, year = {{2000}}, }