Stochastic gene expression in Arabidopsis thaliana
(2017) In Nature Communications 8(1).- Abstract
Although plant development is highly reproducible, some stochasticity exists. This developmental stochasticity may be caused by noisy gene expression. Here we analyze the fluctuation of protein expression in Arabidopsis thaliana. Using the photoconvertible KikGR marker, we show that the protein expressions of individual cells fluctuate over time. A dual reporter system was used to study extrinsic and intrinsic noise of marker gene expression. We report that extrinsic noise is higher than intrinsic noise and that extrinsic noise in stomata is clearly lower in comparison to several other tissues/cell types. Finally, we show that cells are coupled with respect to stochastic protein expression in young leaves, hypocotyls and roots but not... (More)
Although plant development is highly reproducible, some stochasticity exists. This developmental stochasticity may be caused by noisy gene expression. Here we analyze the fluctuation of protein expression in Arabidopsis thaliana. Using the photoconvertible KikGR marker, we show that the protein expressions of individual cells fluctuate over time. A dual reporter system was used to study extrinsic and intrinsic noise of marker gene expression. We report that extrinsic noise is higher than intrinsic noise and that extrinsic noise in stomata is clearly lower in comparison to several other tissues/cell types. Finally, we show that cells are coupled with respect to stochastic protein expression in young leaves, hypocotyls and roots but not in mature leaves. Our data indicate that stochasticity of gene expression can vary between tissues/cell types and that it can be coupled in a non-cell-autonomous manner.
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- author
- Araújo, Ilka Schultheiß ; Pietsch, Jessica Magdalena ; Keizer, Emma Mathilde ; Greese, Bettina LU ; Balkunde, Rachappa ; Fleck, Christian and Hülskamp, Martin
- organization
- publishing date
- 2017-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nature Communications
- volume
- 8
- issue
- 1
- article number
- 2132
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85038836143
- pmid:29242599
- wos:000417906800004
- ISSN
- 2041-1723
- DOI
- 10.1038/s41467-017-02285-7
- language
- English
- LU publication?
- yes
- id
- a46dcff8-b18f-4443-b6b1-670bd17c54c6
- date added to LUP
- 2018-01-04 07:43:44
- date last changed
- 2024-06-10 04:25:29
@article{a46dcff8-b18f-4443-b6b1-670bd17c54c6,
abstract = {{<p>Although plant development is highly reproducible, some stochasticity exists. This developmental stochasticity may be caused by noisy gene expression. Here we analyze the fluctuation of protein expression in Arabidopsis thaliana. Using the photoconvertible KikGR marker, we show that the protein expressions of individual cells fluctuate over time. A dual reporter system was used to study extrinsic and intrinsic noise of marker gene expression. We report that extrinsic noise is higher than intrinsic noise and that extrinsic noise in stomata is clearly lower in comparison to several other tissues/cell types. Finally, we show that cells are coupled with respect to stochastic protein expression in young leaves, hypocotyls and roots but not in mature leaves. Our data indicate that stochasticity of gene expression can vary between tissues/cell types and that it can be coupled in a non-cell-autonomous manner.</p>}},
author = {{Araújo, Ilka Schultheiß and Pietsch, Jessica Magdalena and Keizer, Emma Mathilde and Greese, Bettina and Balkunde, Rachappa and Fleck, Christian and Hülskamp, Martin}},
issn = {{2041-1723}},
language = {{eng}},
month = {{12}},
number = {{1}},
publisher = {{Nature Publishing Group}},
series = {{Nature Communications}},
title = {{Stochastic gene expression in Arabidopsis thaliana}},
url = {{http://dx.doi.org/10.1038/s41467-017-02285-7}},
doi = {{10.1038/s41467-017-02285-7}},
volume = {{8}},
year = {{2017}},
}