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Stochastic gene expression in Arabidopsis thaliana

Araújo, Ilka Schultheiß; Pietsch, Jessica Magdalena; Keizer, Emma Mathilde; Greese, Bettina LU ; Balkunde, Rachappa; Fleck, Christian and Hülskamp, Martin (2017) In Nature Communications 8(1).
Abstract

Although plant development is highly reproducible, some stochasticity exists. This developmental stochasticity may be caused by noisy gene expression. Here we analyze the fluctuation of protein expression in Arabidopsis thaliana. Using the photoconvertible KikGR marker, we show that the protein expressions of individual cells fluctuate over time. A dual reporter system was used to study extrinsic and intrinsic noise of marker gene expression. We report that extrinsic noise is higher than intrinsic noise and that extrinsic noise in stomata is clearly lower in comparison to several other tissues/cell types. Finally, we show that cells are coupled with respect to stochastic protein expression in young leaves, hypocotyls and roots but not... (More)

Although plant development is highly reproducible, some stochasticity exists. This developmental stochasticity may be caused by noisy gene expression. Here we analyze the fluctuation of protein expression in Arabidopsis thaliana. Using the photoconvertible KikGR marker, we show that the protein expressions of individual cells fluctuate over time. A dual reporter system was used to study extrinsic and intrinsic noise of marker gene expression. We report that extrinsic noise is higher than intrinsic noise and that extrinsic noise in stomata is clearly lower in comparison to several other tissues/cell types. Finally, we show that cells are coupled with respect to stochastic protein expression in young leaves, hypocotyls and roots but not in mature leaves. Our data indicate that stochasticity of gene expression can vary between tissues/cell types and that it can be coupled in a non-cell-autonomous manner.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nature Communications
volume
8
issue
1
publisher
Nature Publishing Group
external identifiers
  • scopus:85038836143
  • wos:000417906800004
ISSN
2041-1723
DOI
10.1038/s41467-017-02285-7
language
English
LU publication?
yes
id
a46dcff8-b18f-4443-b6b1-670bd17c54c6
date added to LUP
2018-01-04 07:43:44
date last changed
2018-01-16 13:29:04
@article{a46dcff8-b18f-4443-b6b1-670bd17c54c6,
  abstract     = {<p>Although plant development is highly reproducible, some stochasticity exists. This developmental stochasticity may be caused by noisy gene expression. Here we analyze the fluctuation of protein expression in Arabidopsis thaliana. Using the photoconvertible KikGR marker, we show that the protein expressions of individual cells fluctuate over time. A dual reporter system was used to study extrinsic and intrinsic noise of marker gene expression. We report that extrinsic noise is higher than intrinsic noise and that extrinsic noise in stomata is clearly lower in comparison to several other tissues/cell types. Finally, we show that cells are coupled with respect to stochastic protein expression in young leaves, hypocotyls and roots but not in mature leaves. Our data indicate that stochasticity of gene expression can vary between tissues/cell types and that it can be coupled in a non-cell-autonomous manner.</p>},
  articleno    = {2132},
  author       = {Araújo, Ilka Schultheiß and Pietsch, Jessica Magdalena and Keizer, Emma Mathilde and Greese, Bettina and Balkunde, Rachappa and Fleck, Christian and Hülskamp, Martin},
  issn         = {2041-1723},
  language     = {eng},
  month        = {12},
  number       = {1},
  publisher    = {Nature Publishing Group},
  series       = {Nature Communications},
  title        = {Stochastic gene expression in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1038/s41467-017-02285-7},
  volume       = {8},
  year         = {2017},
}