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Production of moth sex pheromones for pest control by yeast fermentation

Holkenbrink, Carina ; Ding, Bao Jian LU ; Wang, Hong Lei LU ; Dam, Marie Inger LU ; Petkevicius, Karolis ; Kildegaard, Kanchana Rueksomtawin ; Wenning, Leonie ; Sinkwitz, Christina ; Lorántfy, Bettina and Koutsoumpeli, Eleni , et al. (2020) In Metabolic Engineering 62. p.312-321
Abstract

The use of insect sex pheromones is an alternative technology for pest control in agriculture and forestry, which, in contrast to insecticides, does not have adverse effects on human health or environment and is efficient also against insecticide-resistant insect populations. Due to the high cost of chemically synthesized pheromones, mating disruption applications are currently primarily targeting higher value crops, such as fruits. Here we demonstrate a biotechnological method for the production of (Z)-hexadec-11-en-1-ol and (Z)-tetradec-9-en-1-ol, using engineered yeast cell factories. These unsaturated fatty alcohols are pheromone components or the immediate precursors of pheromone components of several economically important moth... (More)

The use of insect sex pheromones is an alternative technology for pest control in agriculture and forestry, which, in contrast to insecticides, does not have adverse effects on human health or environment and is efficient also against insecticide-resistant insect populations. Due to the high cost of chemically synthesized pheromones, mating disruption applications are currently primarily targeting higher value crops, such as fruits. Here we demonstrate a biotechnological method for the production of (Z)-hexadec-11-en-1-ol and (Z)-tetradec-9-en-1-ol, using engineered yeast cell factories. These unsaturated fatty alcohols are pheromone components or the immediate precursors of pheromone components of several economically important moth pests. Biosynthetic pathways towards several pheromones or their precursors were reconstructed in the oleaginous yeast Yarrowia lipolytica, which was further metabolically engineered for improved pheromone biosynthesis by decreasing fatty alcohol degradation and downregulating storage lipid accumulation. The sex pheromone of the cotton bollworm Helicoverpa armigera was produced by oxidation of fermented fatty alcohols into corresponding aldehydes. The resulting yeast-derived pheromone was just as efficient and specific for trapping of H. armigera male moths in cotton fields in Greece as a conventionally produced synthetic pheromone mixture. We further demonstrated the production of (Z)-tetradec-9-en-1-yl acetate, the main pheromone component of the fall armyworm Spodoptera frugiperda. Taken together our work describes a biotech platform for the production of commercially relevant titres of moth pheromones for pest control via yeast fermentation.

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@article{a4e17e68-d2e0-43ba-8e32-8ede2a00b3b0,
  abstract     = {<p>The use of insect sex pheromones is an alternative technology for pest control in agriculture and forestry, which, in contrast to insecticides, does not have adverse effects on human health or environment and is efficient also against insecticide-resistant insect populations. Due to the high cost of chemically synthesized pheromones, mating disruption applications are currently primarily targeting higher value crops, such as fruits. Here we demonstrate a biotechnological method for the production of (Z)-hexadec-11-en-1-ol and (Z)-tetradec-9-en-1-ol, using engineered yeast cell factories. These unsaturated fatty alcohols are pheromone components or the immediate precursors of pheromone components of several economically important moth pests. Biosynthetic pathways towards several pheromones or their precursors were reconstructed in the oleaginous yeast Yarrowia lipolytica, which was further metabolically engineered for improved pheromone biosynthesis by decreasing fatty alcohol degradation and downregulating storage lipid accumulation. The sex pheromone of the cotton bollworm Helicoverpa armigera was produced by oxidation of fermented fatty alcohols into corresponding aldehydes. The resulting yeast-derived pheromone was just as efficient and specific for trapping of H. armigera male moths in cotton fields in Greece as a conventionally produced synthetic pheromone mixture. We further demonstrated the production of (Z)-tetradec-9-en-1-yl acetate, the main pheromone component of the fall armyworm Spodoptera frugiperda. Taken together our work describes a biotech platform for the production of commercially relevant titres of moth pheromones for pest control via yeast fermentation.</p>},
  author       = {Holkenbrink, Carina and Ding, Bao Jian and Wang, Hong Lei and Dam, Marie Inger and Petkevicius, Karolis and Kildegaard, Kanchana Rueksomtawin and Wenning, Leonie and Sinkwitz, Christina and Lorántfy, Bettina and Koutsoumpeli, Eleni and França, Lucas and Pires, Marina and Bernardi, Carmem and Urrutia, William and Mafra-Neto, Agenor and Ferreira, Bruno Sommer and Raptopoulos, Dimitris and Konstantopoulou, Maria and Löfstedt, Christer and Borodina, Irina},
  issn         = {1096-7176},
  language     = {eng},
  pages        = {312--321},
  publisher    = {Elsevier},
  series       = {Metabolic Engineering},
  title        = {Production of moth sex pheromones for pest control by yeast fermentation},
  url          = {http://dx.doi.org/10.1016/j.ymben.2020.10.001},
  doi          = {10.1016/j.ymben.2020.10.001},
  volume       = {62},
  year         = {2020},
}