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Modified ZymoBIOMICS™ DNA/RNA Miniprep Kit for parallel extraction of DNA and RNA from peat soil v1

Mondav, Rhiannon LU orcid (2025)
Abstract
Modified ZYMO DNA/RNA miniprep extraction protocol written in a user friendly way to reduce in-process-decisions and process time. Sample type is organic soil, peat, from a degraded rich fen

This protocol includes two modifications to tackle clogged filters.

Samples were centrifuged for 45 seconds instead of 30 seconds in first pass through filters as 100 ul to 200 ul sample remained trapped above filter with standard protocol
Reduced sample mass to reduce clogging of columns and filters, trialled 200 mg, 150 mg, 100 mg compared to 250 mg.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Working paper/Preprint
publication status
published
subject
publisher
protocols.io
DOI
10.17504/protocols.io.dm6gpmnk8gzp/v1
project
Impact of rewetting drained peatlands on soil microbial activity and composition and its climate mitigation potential
language
English
LU publication?
yes
id
a5d52e12-cccf-4fc3-bb0c-f36145c4af8e
date added to LUP
2025-12-01 15:45:37
date last changed
2025-12-05 16:01:03
@misc{a5d52e12-cccf-4fc3-bb0c-f36145c4af8e,
  abstract     = {{Modified ZYMO DNA/RNA miniprep extraction protocol written in a user friendly way to reduce in-process-decisions and process time. Sample type is organic soil, peat, from a degraded rich fen<br/><br/>This protocol includes two modifications to tackle clogged filters. <br/><br/>Samples were centrifuged for 45 seconds instead of 30 seconds in first pass through filters as 100 ul to 200 ul sample remained trapped above filter with standard protocol<br/>Reduced sample mass to reduce clogging of columns and filters, trialled 200 mg, 150 mg, 100 mg compared to 250 mg.}},
  author       = {{Mondav, Rhiannon}},
  language     = {{eng}},
  month        = {{11}},
  note         = {{Preprint}},
  publisher    = {{protocols.io}},
  title        = {{Modified ZymoBIOMICS™ DNA/RNA Miniprep Kit for parallel extraction of DNA and RNA from peat soil v1}},
  url          = {{http://dx.doi.org/10.17504/protocols.io.dm6gpmnk8gzp/v1}},
  doi          = {{10.17504/protocols.io.dm6gpmnk8gzp/v1}},
  year         = {{2025}},
}