Advanced

Imaging of Metastatic Lymph Nodes by X-ray Phase-Contrast Micro-Tomography

Jensen, Torben Haugaard; Bech, Martin LU ; Binderup, Tina; Boettiger, Arvid; David, Christian; Weitkamp, Timm; Zanette, Irene; Reznikova, Elena; Mohr, Juergen and Rank, Fritz, et al. (2013) In PLoS ONE 8(1).
Abstract
Invasive cancer causes a change in density in the affected tissue, which can be visualized by x-ray phase-contrast tomography. However, the diagnostic value of this method has so far not been investigated in detail. Therefore, the purpose of this study was, in a blinded manner, to investigate whether malignancy could be revealed by non-invasive x-ray phase-contrast tomography in lymph nodes from breast cancer patients. Seventeen formalin-fixed paraffin-embedded lymph nodes from 10 female patients (age range 37-83 years) diagnosed with invasive ductal carcinomas were analyzed by X-ray phase-contrast tomography. Ten lymph nodes had metastatic deposits and 7 were benign. The phase-contrast images were analyzed according to standards for... (More)
Invasive cancer causes a change in density in the affected tissue, which can be visualized by x-ray phase-contrast tomography. However, the diagnostic value of this method has so far not been investigated in detail. Therefore, the purpose of this study was, in a blinded manner, to investigate whether malignancy could be revealed by non-invasive x-ray phase-contrast tomography in lymph nodes from breast cancer patients. Seventeen formalin-fixed paraffin-embedded lymph nodes from 10 female patients (age range 37-83 years) diagnosed with invasive ductal carcinomas were analyzed by X-ray phase-contrast tomography. Ten lymph nodes had metastatic deposits and 7 were benign. The phase-contrast images were analyzed according to standards for conventional CT images looking for characteristics usually only visible by pathological examinations. Histopathology was used as reference. The result of this study was that the diagnostic sensitivity of the image analysis for detecting malignancy was 100% and the specificity was 87%. The positive predictive value was 91% for detecting malignancy and the negative predictive value was 100%. We conclude that x-ray phase-contrast imaging can accurately detect density variations to obtain information regarding lymph node involvement previously inaccessible with standard absorption x-ray imaging. (Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
8
issue
1
publisher
Public Library of Science
external identifiers
  • wos:000313872800023
  • scopus:84872551603
ISSN
1932-6203
DOI
10.1371/journal.pone.0054047
language
English
LU publication?
yes
id
a5fbf93e-6ee5-4ffc-817e-2a3fc957965f (old id 3590716)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23349784
date added to LUP
2013-03-20 14:38:56
date last changed
2019-05-14 02:13:48
@article{a5fbf93e-6ee5-4ffc-817e-2a3fc957965f,
  abstract     = {Invasive cancer causes a change in density in the affected tissue, which can be visualized by x-ray phase-contrast tomography. However, the diagnostic value of this method has so far not been investigated in detail. Therefore, the purpose of this study was, in a blinded manner, to investigate whether malignancy could be revealed by non-invasive x-ray phase-contrast tomography in lymph nodes from breast cancer patients. Seventeen formalin-fixed paraffin-embedded lymph nodes from 10 female patients (age range 37-83 years) diagnosed with invasive ductal carcinomas were analyzed by X-ray phase-contrast tomography. Ten lymph nodes had metastatic deposits and 7 were benign. The phase-contrast images were analyzed according to standards for conventional CT images looking for characteristics usually only visible by pathological examinations. Histopathology was used as reference. The result of this study was that the diagnostic sensitivity of the image analysis for detecting malignancy was 100% and the specificity was 87%. The positive predictive value was 91% for detecting malignancy and the negative predictive value was 100%. We conclude that x-ray phase-contrast imaging can accurately detect density variations to obtain information regarding lymph node involvement previously inaccessible with standard absorption x-ray imaging.},
  author       = {Jensen, Torben Haugaard and Bech, Martin and Binderup, Tina and Boettiger, Arvid and David, Christian and Weitkamp, Timm and Zanette, Irene and Reznikova, Elena and Mohr, Juergen and Rank, Fritz and Feidenhans'l, Robert and Kjaer, Andreas and Hojgaard, Liselotte and Pfeiffer, Franz},
  issn         = {1932-6203},
  language     = {eng},
  number       = {1},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {Imaging of Metastatic Lymph Nodes by X-ray Phase-Contrast Micro-Tomography},
  url          = {http://dx.doi.org/10.1371/journal.pone.0054047},
  volume       = {8},
  year         = {2013},
}