Isolation, crystallisation, and preliminary X-ray analysis of the bovine mitochondrial EF-Tu:GDP and EF-Tu:EF-Ts complexes
(2002) In Biochimica et Biophysica Acta 1601(2). p.172-177- Abstract
- Previous studies have shown that when bovine mitochondrial elongation factor Ts (EF-Ts) is expressed in Escherichia coli, it forms a tightly associated complex with E. coli elongation factor Tu (EF-Tu). In contrast to earlier experiments, purification of free mitochondrial EF-Ts was accomplished under nondenaturing conditions since only about 60% of the expressed EF-Ts copurified with E. coli EF-Tu. The bovine mitochondrial EF-Tu:GDP complex, the homologous mitochondrial EF-Tu:EF-Ts complex, and the heterologous E. coli/mitochondrial EF-Tu:EF-Ts complex were isolated and crystallised. The crystals of the EF-Tu:GDP complex diffract to 1.94 A and belong to space group P2(1) with cell parameters a=59.09 A, b=119.78 A, c=128.89 A and... (More)
- Previous studies have shown that when bovine mitochondrial elongation factor Ts (EF-Ts) is expressed in Escherichia coli, it forms a tightly associated complex with E. coli elongation factor Tu (EF-Tu). In contrast to earlier experiments, purification of free mitochondrial EF-Ts was accomplished under nondenaturing conditions since only about 60% of the expressed EF-Ts copurified with E. coli EF-Tu. The bovine mitochondrial EF-Tu:GDP complex, the homologous mitochondrial EF-Tu:EF-Ts complex, and the heterologous E. coli/mitochondrial EF-Tu:EF-Ts complex were isolated and crystallised. The crystals of the EF-Tu:GDP complex diffract to 1.94 A and belong to space group P2(1) with cell parameters a=59.09 A, b=119.78 A, c=128.89 A and beta=96.978 degrees. The crystals of the homologous mitochondrial EF-Tu:EF-Ts complex diffract to 4 A and belong to space group C2 with cell parameters a=157.7 A, b=151.9 A, c=156.9 A, and beta=108.96 degrees. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1125392
- author
- Karring, Henrik LU ; Andersen, Gregers R ; Thirup, Soren S ; Nyborg, Jens ; Spremulli, Linda L and Clark, Brian F C
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochimica et Biophysica Acta
- volume
- 1601
- issue
- 2
- pages
- 172 - 177
- publisher
- Elsevier
- external identifiers
-
- pmid:12445479
- scopus:0037121462
- ISSN
- 0006-3002
- DOI
- 10.1016/S1570-9639(02)00460-0
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Connective Tissue Biology (013230151)
- id
- a6340c4c-3ebc-47fc-bd98-8a5860997e92 (old id 1125392)
- date added to LUP
- 2016-04-01 15:21:48
- date last changed
- 2022-01-28 04:59:40
@article{a6340c4c-3ebc-47fc-bd98-8a5860997e92,
abstract = {{Previous studies have shown that when bovine mitochondrial elongation factor Ts (EF-Ts) is expressed in Escherichia coli, it forms a tightly associated complex with E. coli elongation factor Tu (EF-Tu). In contrast to earlier experiments, purification of free mitochondrial EF-Ts was accomplished under nondenaturing conditions since only about 60% of the expressed EF-Ts copurified with E. coli EF-Tu. The bovine mitochondrial EF-Tu:GDP complex, the homologous mitochondrial EF-Tu:EF-Ts complex, and the heterologous E. coli/mitochondrial EF-Tu:EF-Ts complex were isolated and crystallised. The crystals of the EF-Tu:GDP complex diffract to 1.94 A and belong to space group P2(1) with cell parameters a=59.09 A, b=119.78 A, c=128.89 A and beta=96.978 degrees. The crystals of the homologous mitochondrial EF-Tu:EF-Ts complex diffract to 4 A and belong to space group C2 with cell parameters a=157.7 A, b=151.9 A, c=156.9 A, and beta=108.96 degrees.}},
author = {{Karring, Henrik and Andersen, Gregers R and Thirup, Soren S and Nyborg, Jens and Spremulli, Linda L and Clark, Brian F C}},
issn = {{0006-3002}},
language = {{eng}},
number = {{2}},
pages = {{172--177}},
publisher = {{Elsevier}},
series = {{Biochimica et Biophysica Acta}},
title = {{Isolation, crystallisation, and preliminary X-ray analysis of the bovine mitochondrial EF-Tu:GDP and EF-Tu:EF-Ts complexes}},
url = {{http://dx.doi.org/10.1016/S1570-9639(02)00460-0}},
doi = {{10.1016/S1570-9639(02)00460-0}},
volume = {{1601}},
year = {{2002}},
}