Site-specific DNA-controlled fusion of single lipid vesicles to supported lipid bilayers
(2010) In ChemPhysChem 11(5). p.7-1011- Abstract
We investigate the Ca(2+)-triggered fusion of lipid vesicles site-selectively tethered to a DNA-modified supported lipid bilayer array, with the DNA strands designed such that hybridization occurs in a zipperlike fashion. Prior to the addition of Ca(2+), which is observed to induce docking and subsequent fusion (within 200 ms), the vesicles display lateral mobility determined by the number of tethers. Fusion is observed to require around ten DNA strands per vesicle, but does not occur at higher DNA coverage. However, despite the fact that fusion was restricted to occurring for vesicles tethered with around ten DNA strands, there is no correlation between single-vesicle diffusivity and fusogenicity. A possible scenario for the... (More)
We investigate the Ca(2+)-triggered fusion of lipid vesicles site-selectively tethered to a DNA-modified supported lipid bilayer array, with the DNA strands designed such that hybridization occurs in a zipperlike fashion. Prior to the addition of Ca(2+), which is observed to induce docking and subsequent fusion (within 200 ms), the vesicles display lateral mobility determined by the number of tethers. Fusion is observed to require around ten DNA strands per vesicle, but does not occur at higher DNA coverage. However, despite the fact that fusion was restricted to occurring for vesicles tethered with around ten DNA strands, there is no correlation between single-vesicle diffusivity and fusogenicity. A possible scenario for the DNA-induced fusion machinery, consistent with these observations, is that prior to Ca(2+)-induced docking, the vesicles diffuse with a small number (2-4) of DNA tethers. Upon addition of Ca(2+), the vesicles dock, presumably due to bridging of lipid head groups. Fusion then occurs under conditions where 10-16 DNA tethers form and rearrange at the rim of the contact region between a docked vesicle and the SLB. The time required for this rearrangement, which may include both DNA hybridization and dehybridization during zipping, is expected to represent the observed docking and fusion time of less than 200 ms.
(Less)
- author
- Simonsson, Lisa LU ; Jönsson, Peter LU ; Stengel, Gudrun LU and Höök, Fredrik LU
- publishing date
- 2010-04-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- DNA, Lipid Bilayers, Membrane Fluidity, Microfluidic Analytical Techniques, SNARE Proteins, Journal Article, Research Support, Non-U.S. Gov't
- in
- ChemPhysChem
- volume
- 11
- issue
- 5
- pages
- 7 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:20301177
- scopus:77950346996
- ISSN
- 1439-7641
- DOI
- 10.1002/cphc.200901010
- language
- English
- LU publication?
- no
- id
- a699c3fb-692b-4de9-a4d6-37f68b507049
- date added to LUP
- 2018-01-26 10:50:31
- date last changed
- 2024-06-24 09:08:32
@article{a699c3fb-692b-4de9-a4d6-37f68b507049,
abstract = {{<p>We investigate the Ca(2+)-triggered fusion of lipid vesicles site-selectively tethered to a DNA-modified supported lipid bilayer array, with the DNA strands designed such that hybridization occurs in a zipperlike fashion. Prior to the addition of Ca(2+), which is observed to induce docking and subsequent fusion (within 200 ms), the vesicles display lateral mobility determined by the number of tethers. Fusion is observed to require around ten DNA strands per vesicle, but does not occur at higher DNA coverage. However, despite the fact that fusion was restricted to occurring for vesicles tethered with around ten DNA strands, there is no correlation between single-vesicle diffusivity and fusogenicity. A possible scenario for the DNA-induced fusion machinery, consistent with these observations, is that prior to Ca(2+)-induced docking, the vesicles diffuse with a small number (2-4) of DNA tethers. Upon addition of Ca(2+), the vesicles dock, presumably due to bridging of lipid head groups. Fusion then occurs under conditions where 10-16 DNA tethers form and rearrange at the rim of the contact region between a docked vesicle and the SLB. The time required for this rearrangement, which may include both DNA hybridization and dehybridization during zipping, is expected to represent the observed docking and fusion time of less than 200 ms.</p>}},
author = {{Simonsson, Lisa and Jönsson, Peter and Stengel, Gudrun and Höök, Fredrik}},
issn = {{1439-7641}},
keywords = {{DNA; Lipid Bilayers; Membrane Fluidity; Microfluidic Analytical Techniques; SNARE Proteins; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
month = {{04}},
number = {{5}},
pages = {{7--1011}},
publisher = {{John Wiley & Sons Inc.}},
series = {{ChemPhysChem}},
title = {{Site-specific DNA-controlled fusion of single lipid vesicles to supported lipid bilayers}},
url = {{http://dx.doi.org/10.1002/cphc.200901010}},
doi = {{10.1002/cphc.200901010}},
volume = {{11}},
year = {{2010}},
}