Steroid glycosides isolated from Paris polyphylla var. chinensis aerial parts and paris saponin II induces G1/S-phase MCF-7 cell cycle arrest
(2022) In Carbohydrate Research 519.- Abstract
In our previous research on Vietnamese medicinal plants, we found that the ethanolic extract of the aerial parts of Paris polyphylla var. chinensis exhibited cytotoxic effects in vitro in the MCF-7 human cancer cell line. Here, we used combined chromatographic separations to isolate six compounds including a new steroid glycoside, paripoloside A (3), and five known compounds, from the butanol extract of the aerial parts of P. polyphylla. We unambiguously elucidated their structures based on spectroscopic data (proton and carbon-13 nuclear magnetic resonance, heteronuclear single quantum coherence, heteronuclear multiple bond correlation, correlation spectroscopy, and high-resolution electrospray ionization mass spectroscopy data), and... (More)
In our previous research on Vietnamese medicinal plants, we found that the ethanolic extract of the aerial parts of Paris polyphylla var. chinensis exhibited cytotoxic effects in vitro in the MCF-7 human cancer cell line. Here, we used combined chromatographic separations to isolate six compounds including a new steroid glycoside, paripoloside A (3), and five known compounds, from the butanol extract of the aerial parts of P. polyphylla. We unambiguously elucidated their structures based on spectroscopic data (proton and carbon-13 nuclear magnetic resonance, heteronuclear single quantum coherence, heteronuclear multiple bond correlation, correlation spectroscopy, and high-resolution electrospray ionization mass spectroscopy data), and chemical reactions. Among the isolated compounds, paris saponin II (PSII) had the strongest cytotoxic effects against MCF-7 breast cancer cells. Interestingly, PSII significantly increased the expression of p53, p21, p27, and Bax protein levels and significantly suppressed the expression of cyclin D1 and retinoblastoma protein. These data suggest that PSII may induce G1/S phase cell cycle arrest and apoptosis pathway development in MCF-7 cells. Furthermore, the MCF-7 breast cancer cells mechanism of PSII was also investigated using molecular docking. Together, our results demonstrate that isolated compounds from P. polyphylla are promising candidates as breast cancer inhibitors.
(Less)
- author
- Duyen, Nguyen Thi ; Vinh, Le Ba ; Phong, Nguyen Viet ; Khoi, Nguyen Minh ; Ha, Do Thi ; Long, Pham Quoc ; Dung, Le Viet ; Hien, Tran Thi LU ; Dat, Nguyen Tien and Lee, Ki Yong
- organization
- publishing date
- 2022-09
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Breast cancer, Cytotoxic activity, Paripoloside A, Paris polyphylla var. chinensis, Steroid glycosides
- in
- Carbohydrate Research
- volume
- 519
- article number
- 108613
- publisher
- Elsevier
- external identifiers
-
- scopus:85132508692
- pmid:35752103
- ISSN
- 0008-6215
- DOI
- 10.1016/j.carres.2022.108613
- language
- English
- LU publication?
- yes
- id
- a6a47ac3-e6f8-447c-b1e1-c8171862bfa5
- date added to LUP
- 2022-09-26 16:15:41
- date last changed
- 2024-06-13 15:06:35
@article{a6a47ac3-e6f8-447c-b1e1-c8171862bfa5,
abstract = {{<p>In our previous research on Vietnamese medicinal plants, we found that the ethanolic extract of the aerial parts of Paris polyphylla var. chinensis exhibited cytotoxic effects in vitro in the MCF-7 human cancer cell line. Here, we used combined chromatographic separations to isolate six compounds including a new steroid glycoside, paripoloside A (3), and five known compounds, from the butanol extract of the aerial parts of P. polyphylla. We unambiguously elucidated their structures based on spectroscopic data (proton and carbon-13 nuclear magnetic resonance, heteronuclear single quantum coherence, heteronuclear multiple bond correlation, correlation spectroscopy, and high-resolution electrospray ionization mass spectroscopy data), and chemical reactions. Among the isolated compounds, paris saponin II (PSII) had the strongest cytotoxic effects against MCF-7 breast cancer cells. Interestingly, PSII significantly increased the expression of p53, p21, p27, and Bax protein levels and significantly suppressed the expression of cyclin D1 and retinoblastoma protein. These data suggest that PSII may induce G1/S phase cell cycle arrest and apoptosis pathway development in MCF-7 cells. Furthermore, the MCF-7 breast cancer cells mechanism of PSII was also investigated using molecular docking. Together, our results demonstrate that isolated compounds from P. polyphylla are promising candidates as breast cancer inhibitors.</p>}},
author = {{Duyen, Nguyen Thi and Vinh, Le Ba and Phong, Nguyen Viet and Khoi, Nguyen Minh and Ha, Do Thi and Long, Pham Quoc and Dung, Le Viet and Hien, Tran Thi and Dat, Nguyen Tien and Lee, Ki Yong}},
issn = {{0008-6215}},
keywords = {{Breast cancer; Cytotoxic activity; Paripoloside A; Paris polyphylla var. chinensis; Steroid glycosides}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{Carbohydrate Research}},
title = {{Steroid glycosides isolated from Paris polyphylla var. chinensis aerial parts and paris saponin II induces G1/S-phase MCF-7 cell cycle arrest}},
url = {{http://dx.doi.org/10.1016/j.carres.2022.108613}},
doi = {{10.1016/j.carres.2022.108613}},
volume = {{519}},
year = {{2022}},
}