Affinity Two-Phase Partitioning in Acoustically Levitated Drops
(2004) In Analytical Chemistry 76(2). p.303-308- Abstract
- Miniaturized (<1 L) biospecific affinity two-phase partitioning in an acoustically levitated drop is described. Miniaturization commonly gives unfavorable surface/volume ratios, but in the levitation approach adsorption problems are minimized since the only surrounding wall is the liquid/air interface of the drop. Biotinylated liposomes were partitioned in aqueous poly(ethylene glycol)/dextran two-phase drops with NeutrAvidin-dextran as the affinity ligand. A two-phase drop was trapped and manipulated in a node of a standing ultrasonic wave. Alternatively, a two-phase system was formed by levitation and evaporation of a polymer one-phase drop. Phase mixing was achieved by adjusting the ultrasonic field and phase separation by... (More)
- Miniaturized (<1 L) biospecific affinity two-phase partitioning in an acoustically levitated drop is described. Miniaturization commonly gives unfavorable surface/volume ratios, but in the levitation approach adsorption problems are minimized since the only surrounding wall is the liquid/air interface of the drop. Biotinylated liposomes were partitioned in aqueous poly(ethylene glycol)/dextran two-phase drops with NeutrAvidin-dextran as the affinity ligand. A two-phase drop was trapped and manipulated in a node of a standing ultrasonic wave. Alternatively, a two-phase system was formed by levitation and evaporation of a polymer one-phase drop. Phase mixing was achieved by adjusting the ultrasonic field and phase separation by readjusting the field. NeutrAvidin-dextran brought about the redistribution of biotinylated liposomes from the poly(ethylene glycol)-rich phase into the dextran-rich phase. Thus, an entire affinity two-phase separation procedure, including mixing of the phases and incubation to allow affinity interactions to develop under constant volume, followed by phase separation under controlled evaporation, can be performed in a single levitated drop. This miniaturized technique would allow the separation of biologically active membranes or organelles from individual cells for analysis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/126105
- author
- Santesson, Sabina LU ; Barinaga-Rementeria Ramírez, Irene LU ; Viberg, Peter LU ; Jergil, Bengt LU and Nilsson, Staffan LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 76
- issue
- 2
- pages
- 303 - 308
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:14719875
- wos:000188198800008
- scopus:0346459923
- pmid:14719875
- ISSN
- 1520-6882
- DOI
- 10.1021/ac034951h
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Biochemistry and Structural Biology (S) (000006142)
- id
- a6b42434-befc-4044-8056-3158dc1c3420 (old id 126105)
- date added to LUP
- 2016-04-04 12:10:45
- date last changed
- 2022-03-16 00:24:42
@article{a6b42434-befc-4044-8056-3158dc1c3420, abstract = {{Miniaturized (<1 L) biospecific affinity two-phase partitioning in an acoustically levitated drop is described. Miniaturization commonly gives unfavorable surface/volume ratios, but in the levitation approach adsorption problems are minimized since the only surrounding wall is the liquid/air interface of the drop. Biotinylated liposomes were partitioned in aqueous poly(ethylene glycol)/dextran two-phase drops with NeutrAvidin-dextran as the affinity ligand. A two-phase drop was trapped and manipulated in a node of a standing ultrasonic wave. Alternatively, a two-phase system was formed by levitation and evaporation of a polymer one-phase drop. Phase mixing was achieved by adjusting the ultrasonic field and phase separation by readjusting the field. NeutrAvidin-dextran brought about the redistribution of biotinylated liposomes from the poly(ethylene glycol)-rich phase into the dextran-rich phase. Thus, an entire affinity two-phase separation procedure, including mixing of the phases and incubation to allow affinity interactions to develop under constant volume, followed by phase separation under controlled evaporation, can be performed in a single levitated drop. This miniaturized technique would allow the separation of biologically active membranes or organelles from individual cells for analysis.}}, author = {{Santesson, Sabina and Barinaga-Rementeria Ramírez, Irene and Viberg, Peter and Jergil, Bengt and Nilsson, Staffan}}, issn = {{1520-6882}}, language = {{eng}}, number = {{2}}, pages = {{303--308}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Analytical Chemistry}}, title = {{Affinity Two-Phase Partitioning in Acoustically Levitated Drops}}, url = {{http://dx.doi.org/10.1021/ac034951h}}, doi = {{10.1021/ac034951h}}, volume = {{76}}, year = {{2004}}, }