Advanced

Fast and easy coating for capillary electrophoresis based on a physically adsorbed cationic copolymer

Bernal, José; Rodríguez-Meizoso, Irene LU ; Elvira, Carlos; Ibáñez, Elena and Cifuentes, Alejandro (2008) In Journal of Chromatography A 1204(1). p.104-109
Abstract

In this work, a new copolymer synthesized in our laboratory is used as physically adsorbed coating for capillary electrophoresis (CE). The copolymer is composed of ethylpyrrolidine methacrylate (EPyM) and methylmethacrylate (MMA). The capillary coating is easily obtained by simply flushing into the tubing an EPyM/MMA solution. It is demonstrated that the composition of the EPyM/MMA copolymer together with the selection of the background electrolyte (BGE) and pH allow tailoring the direction and magnitude of the electroosmotic flow (EOF) in CE. It is also shown that the EOF obtained for the EPyM/MMA-coated capillaries was reproducible in all cases independently on pH or polymer composition. Thus, RSD values lower than 1.9% (n = 5) for... (More)

In this work, a new copolymer synthesized in our laboratory is used as physically adsorbed coating for capillary electrophoresis (CE). The copolymer is composed of ethylpyrrolidine methacrylate (EPyM) and methylmethacrylate (MMA). The capillary coating is easily obtained by simply flushing into the tubing an EPyM/MMA solution. It is demonstrated that the composition of the EPyM/MMA copolymer together with the selection of the background electrolyte (BGE) and pH allow tailoring the direction and magnitude of the electroosmotic flow (EOF) in CE. It is also shown that the EOF obtained for the EPyM/MMA-coated capillaries was reproducible in all cases independently on pH or polymer composition. Thus, RSD values lower than 1.9% (n = 5) for the same capillary and day were obtained for the migration time, while the repeatability interdays (n = 5) was observed to provide RSD values lower than 0.5%. The stability of the coating procedure was also tested between capillaries (n = 3) obtaining RSD values lower than 0.6%. It is demonstrated with several examples that the use of EPyM/MMA coatings in CE can drastically reduce the analysis time and/or to improve the resolution of the separations. It is shown that EPyM/MMA-coated capillaries allow the separation of basic proteins by reducing their adsorption onto the capillary wall. Also, EPyM/MMA-coated capillaries provide a faster separation of samples containing simultaneously positive and negative analytes. Moreover, it is demonstrated that the use of EPyM/MMA-coated capillaries can incorporate an additional chromatographic-like interaction with nucleosides that highly improves the separation of this group of solutes. © 2008 Elsevier B.V. All rights reserved.

(Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
keywords
Amino acids, Benzoic acids, Coated capillaries, Nucleosides, Physically adsorbed polymers, Proteins
in
Journal of Chromatography A
volume
1204
issue
1
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:49549095977
ISSN
0021-9673
DOI
10.1016/j.chroma.2008.07.061
language
English
LU publication?
no
id
a6d146c0-e19b-4bef-9673-b5feef41b74d
date added to LUP
2017-04-10 10:35:52
date last changed
2017-05-29 15:26:53
@article{a6d146c0-e19b-4bef-9673-b5feef41b74d,
  abstract     = {<p>In this work, a new copolymer synthesized in our laboratory is used as physically adsorbed coating for capillary electrophoresis (CE). The copolymer is composed of ethylpyrrolidine methacrylate (EPyM) and methylmethacrylate (MMA). The capillary coating is easily obtained by simply flushing into the tubing an EPyM/MMA solution. It is demonstrated that the composition of the EPyM/MMA copolymer together with the selection of the background electrolyte (BGE) and pH allow tailoring the direction and magnitude of the electroosmotic flow (EOF) in CE. It is also shown that the EOF obtained for the EPyM/MMA-coated capillaries was reproducible in all cases independently on pH or polymer composition. Thus, RSD values lower than 1.9% (n = 5) for the same capillary and day were obtained for the migration time, while the repeatability interdays (n = 5) was observed to provide RSD values lower than 0.5%. The stability of the coating procedure was also tested between capillaries (n = 3) obtaining RSD values lower than 0.6%. It is demonstrated with several examples that the use of EPyM/MMA coatings in CE can drastically reduce the analysis time and/or to improve the resolution of the separations. It is shown that EPyM/MMA-coated capillaries allow the separation of basic proteins by reducing their adsorption onto the capillary wall. Also, EPyM/MMA-coated capillaries provide a faster separation of samples containing simultaneously positive and negative analytes. Moreover, it is demonstrated that the use of EPyM/MMA-coated capillaries can incorporate an additional chromatographic-like interaction with nucleosides that highly improves the separation of this group of solutes. © 2008 Elsevier B.V. All rights reserved.</p>},
  author       = {Bernal, José and Rodríguez-Meizoso, Irene and Elvira, Carlos and Ibáñez, Elena and Cifuentes, Alejandro},
  issn         = {0021-9673},
  keyword      = {Amino acids,Benzoic acids,Coated capillaries,Nucleosides,Physically adsorbed polymers,Proteins},
  language     = {eng},
  month        = {09},
  number       = {1},
  pages        = {104--109},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Fast and easy coating for capillary electrophoresis based on a physically adsorbed cationic copolymer},
  url          = {http://dx.doi.org/10.1016/j.chroma.2008.07.061},
  volume       = {1204},
  year         = {2008},
}