Effect of Aurora kinase B on polyploidy and decidualization in mouse uterus
Wang, Peng Chao ; Yang, Zhen Shan LU
and Gu, Xiao Wei
(2023)
In American Journal of Reproductive Immunology
90(5).
- Abstract
Research question: Decidualization is critical to the establishment of mouse normal pregnancy. The fibroblast-like stromal cells in the process form polyploid multinucleated cells. Aurora kinase B (Aurora B) has previously been shown to regulate polyploidy in various cells. However, whether Aurora B regulates the formation of decidual cell polyploidization and its regulatory mechanisms remain poorly understood. Design:: Establish decidualization model of mouse primary endometrial stromal cells in vitro. Construct pseudopregnancy mouse models and delayed-activation mouse models. Detect Aurora B and polyploidization related genes in mouse uteri treated by Aurora B specific inhibitor Barasertib and CPT. Results:: In this study, we found... (More)
Research question: Decidualization is critical to the establishment of mouse normal pregnancy. The fibroblast-like stromal cells in the process form polyploid multinucleated cells. Aurora kinase B (Aurora B) has previously been shown to regulate polyploidy in various cells. However, whether Aurora B regulates the formation of decidual cell polyploidization and its regulatory mechanisms remain poorly understood. Design:: Establish decidualization model of mouse primary endometrial stromal cells in vitro. Construct pseudopregnancy mouse models and delayed-activation mouse models. Detect Aurora B and polyploidization related genes in mouse uteri treated by Aurora B specific inhibitor Barasertib and CPT. Results:: In this study, we found that Aurora B was strongly expressed in endometrial stromal cells after implantation. Additionally, Aurora B was remarkably up regulated in the stromal cells of oil-induced deciduomoa and in vitro decidualization. As an Aurora B specific inhibitor, Barasertib significantly inhibits the mRNA expression of Prl8a2, a marker of mouse decidualization. Furthermore, the protein levels of p-Plk1, Survivin and p-Cdk1 were inhibited by Barasertib. CPT-induced DNA damage suppressed Aurkb (encodes Aurora B) expression, thus resulting in polyploidization. Conclusion:: Our data shows that Aurora B is expressed in decidual stromal cells of implantation sites and plays a key role for mouse decidualization. The protein of Plk1, Survivn, and Cdk1 may participate in formation of decidual cell polyploidization during mouse decidualization.
(Less)
- author
- Wang, Peng Chao
; Yang, Zhen Shan
LU
and Gu, Xiao Wei
- organization
- publishing date
- 2023-11
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aurora B, decidualization, polyploidization, uterus
- in
- American Journal of Reproductive Immunology
- volume
- 90
- issue
- 5
- article number
- e13793
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:37881124
- scopus:85174828664
- ISSN
- 1046-7408
- DOI
- 10.1111/aji.13793
- language
- English
- LU publication?
- yes
- id
- a6d4118e-f682-4d89-a0d3-1e32182b925a
- date added to LUP
- 2023-12-11 13:34:56
- date last changed
- 2024-04-24 07:02:22
@article{a6d4118e-f682-4d89-a0d3-1e32182b925a,
abstract = {{<p>Research question: Decidualization is critical to the establishment of mouse normal pregnancy. The fibroblast-like stromal cells in the process form polyploid multinucleated cells. Aurora kinase B (Aurora B) has previously been shown to regulate polyploidy in various cells. However, whether Aurora B regulates the formation of decidual cell polyploidization and its regulatory mechanisms remain poorly understood. Design:: Establish decidualization model of mouse primary endometrial stromal cells in vitro. Construct pseudopregnancy mouse models and delayed-activation mouse models. Detect Aurora B and polyploidization related genes in mouse uteri treated by Aurora B specific inhibitor Barasertib and CPT. Results:: In this study, we found that Aurora B was strongly expressed in endometrial stromal cells after implantation. Additionally, Aurora B was remarkably up regulated in the stromal cells of oil-induced deciduomoa and in vitro decidualization. As an Aurora B specific inhibitor, Barasertib significantly inhibits the mRNA expression of Prl8a2, a marker of mouse decidualization. Furthermore, the protein levels of p-Plk1, Survivin and p-Cdk1 were inhibited by Barasertib. CPT-induced DNA damage suppressed Aurkb (encodes Aurora B) expression, thus resulting in polyploidization. Conclusion:: Our data shows that Aurora B is expressed in decidual stromal cells of implantation sites and plays a key role for mouse decidualization. The protein of Plk1, Survivn, and Cdk1 may participate in formation of decidual cell polyploidization during mouse decidualization.</p>}},
author = {{Wang, Peng Chao and Yang, Zhen Shan and Gu, Xiao Wei}},
issn = {{1046-7408}},
keywords = {{Aurora B; decidualization; polyploidization; uterus}},
language = {{eng}},
number = {{5}},
publisher = {{Wiley-Blackwell}},
series = {{American Journal of Reproductive Immunology}},
title = {{Effect of Aurora kinase B on polyploidy and decidualization in mouse uterus}},
url = {{http://dx.doi.org/10.1111/aji.13793}},
doi = {{10.1111/aji.13793}},
volume = {{90}},
year = {{2023}},
}