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Quantitative analysis of islet glutamic acid decarboxylase p64 autoantibodies in insulin-dependent diabetes mellitus

Bärmeier, Heike ; Ahlmén, Jarl ; Landin-Olsson, Mono LU ; Rajotte, Ray V. ; Sundkvist, Göran LU ; Warnock, Garth and Lernmark, Åke LU orcid (1992) In Autoimmunity 13(3). p.187-196
Abstract

Autoantibodies against the βcell Mr 64,000 protein (p64), recently identified as an isoform of glutamic acid decarboxylase (GAD), are prevalent in patients with insulin-dependent diabetes mellitus (IDDM). Dog islets were found to represent an abundant source of native p64 allowing the study of antigen-antibody interactions in IDDM. A quantitative, standardized assay for p64 antibodies based on dog islets was developed and evaluated. Utilizing dog and human islets the p64 antibodies were detected in 17/19 (89%) new onset 15-32-year-old patients, compared to 15/19 (79%) in a rat islet assay. ICA were detected in 15/19 (79%) patients and correlated with the presence of p64 antibodies (rs=0.59, P < 0.004) but not with age at... (More)

Autoantibodies against the βcell Mr 64,000 protein (p64), recently identified as an isoform of glutamic acid decarboxylase (GAD), are prevalent in patients with insulin-dependent diabetes mellitus (IDDM). Dog islets were found to represent an abundant source of native p64 allowing the study of antigen-antibody interactions in IDDM. A quantitative, standardized assay for p64 antibodies based on dog islets was developed and evaluated. Utilizing dog and human islets the p64 antibodies were detected in 17/19 (89%) new onset 15-32-year-old patients, compared to 15/19 (79%) in a rat islet assay. ICA were detected in 15/19 (79%) patients and correlated with the presence of p64 antibodies (rs=0.59, P < 0.004) but not with age at onset, sex, or C-peptide levels. Sensitivity therefore is improved with the dog islet p64 antibody assay which will allow future studies requiring native p64 antigen in larger quantities are possible based on our findings.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Autoantibodies, Autoantigen, Dog islets, Immunoprecipitation, Islet cell antibodies
in
Autoimmunity
volume
13
issue
3
pages
187 - 196
publisher
Taylor & Francis
external identifiers
  • pmid:1472630
  • scopus:0026463519
ISSN
0891-6934
DOI
10.3109/08916939209004823
language
English
LU publication?
no
id
a6ecd08c-7a2e-4090-8126-d704208fff7e
date added to LUP
2019-09-11 09:34:11
date last changed
2024-03-13 08:10:31
@article{a6ecd08c-7a2e-4090-8126-d704208fff7e,
  abstract     = {{<p>Autoantibodies against the βcell Mr 64,000 protein (p64), recently identified as an isoform of glutamic acid decarboxylase (GAD), are prevalent in patients with insulin-dependent diabetes mellitus (IDDM). Dog islets were found to represent an abundant source of native p64 allowing the study of antigen-antibody interactions in IDDM. A quantitative, standardized assay for p64 antibodies based on dog islets was developed and evaluated. Utilizing dog and human islets the p64 antibodies were detected in 17/19 (89%) new onset 15-32-year-old patients, compared to 15/19 (79%) in a rat islet assay. ICA were detected in 15/19 (79%) patients and correlated with the presence of p64 antibodies (r<sub>s</sub>=0.59, P &lt; 0.004) but not with age at onset, sex, or C-peptide levels. Sensitivity therefore is improved with the dog islet p64 antibody assay which will allow future studies requiring native p64 antigen in larger quantities are possible based on our findings.</p>}},
  author       = {{Bärmeier, Heike and Ahlmén, Jarl and Landin-Olsson, Mono and Rajotte, Ray V. and Sundkvist, Göran and Warnock, Garth and Lernmark, Åke}},
  issn         = {{0891-6934}},
  keywords     = {{Autoantibodies; Autoantigen; Dog islets; Immunoprecipitation; Islet cell antibodies}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{3}},
  pages        = {{187--196}},
  publisher    = {{Taylor & Francis}},
  series       = {{Autoimmunity}},
  title        = {{Quantitative analysis of islet glutamic acid decarboxylase p64 autoantibodies in insulin-dependent diabetes mellitus}},
  url          = {{http://dx.doi.org/10.3109/08916939209004823}},
  doi          = {{10.3109/08916939209004823}},
  volume       = {{13}},
  year         = {{1992}},
}