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Rapid and facile purification of apolipoprotein A-I from human plasma using thermoresponsive nanoparticles

Lundqvist, Martin LU ; Berggård, Tord LU ; Hellstrand, Erik LU ; Lynch, Iseult LU ; Dawson, Kenneth A ; Linse, Sara LU and Cedervall, Tommy LU (2011) In Journal of Biomaterials and Nanobiotechnology 2(3). p.258-266
Abstract
Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized protocol using 50:50 NiPAM:BAM copolymer nanoparticles with thermo-responsive properties as an affinity resin. Repeated pelleting and washing of nanoparticle-captured apoA-I is achieved through temperature cycling. The protein is then eluted using urea followed by an ion exchange step for protein concentration and depletion of nanoparticles
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biomaterials and Nanobiotechnology
volume
2
issue
3
pages
9 pages
publisher
Scientific Research Publishing (SCIRP)
ISSN
2158-7027
DOI
10.4236/jbnb.2011.23033
language
English
LU publication?
yes
id
a75ca6eb-72bf-46c7-87f9-95953621d31f
date added to LUP
2021-10-19 13:14:42
date last changed
2021-10-25 09:46:13
@article{a75ca6eb-72bf-46c7-87f9-95953621d31f,
  abstract     = {{Nanoparticles can be used to purify proteins from plasma. We report here the purification of apolipoprotein A-I (apoA-I) with high specificity from human plasma using copolymeric nanoparticles. We present an optimized protocol using 50:50 NiPAM:BAM copolymer nanoparticles with thermo-responsive properties as an affinity resin. Repeated pelleting and washing of nanoparticle-captured apoA-I is achieved through temperature cycling. The protein is then eluted using urea followed by an ion exchange step for protein concentration and depletion of nanoparticles}},
  author       = {{Lundqvist, Martin and Berggård, Tord and Hellstrand, Erik and Lynch, Iseult and Dawson, Kenneth A and Linse, Sara and Cedervall, Tommy}},
  issn         = {{2158-7027}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{258--266}},
  publisher    = {{Scientific Research Publishing (SCIRP)}},
  series       = {{Journal of Biomaterials and Nanobiotechnology}},
  title        = {{Rapid and facile purification of apolipoprotein A-I from human plasma using thermoresponsive nanoparticles}},
  url          = {{http://dx.doi.org/10.4236/jbnb.2011.23033}},
  doi          = {{10.4236/jbnb.2011.23033}},
  volume       = {{2}},
  year         = {{2011}},
}